Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro     

Dolmatova L. S.  Eliseykina M. G.  Timchenko N. F.  Kovaleva A. L.  Shitkova O. A. 《中国海洋湖沼学报》2003,21(4):293-304

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).  相似文献   

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone     

李新正  刘瑞玉 《中国海洋湖沼学报》2003,21(2):154-165

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.  相似文献   

A taxonomical and ecological survey of rotiffer communities in Krottensee (Austria)     

Zhu Ge-yan 《中国海洋湖沼学报》1996,14(4):308-315

The composition and distribution of benthic, periphytic and planktonic rotifers in different habitats in Krottensee were investigated. Sixty-five rotifer taxa were identified, their relative abundance estimated. Classification of the data set by cluster analysis allowed the recognition of distinct habitat groups confined to acid bog ponds, macrophytes, sediments and open water. Five groups of rotifer taxa were identified on the basis of their habitat preferences and the occurrence of the taxa. Highest diversities were found on macrophytes and in acid bog ponds.  相似文献   

On a new species ofPavlova (Prymnesiophyceae) from China     

C. K. Tseng  Chen Jiaofen  Zhang Zhefu 《中国海洋湖沼学报》1992,10(1):23-30

Pavlova viridis sp. nov. is described on the basis of light and electron microscope observations. The material was collected from the coast of haiyang county of Shandong, China. It is characterized by 1) yellowish green to green chloroplast, 2) rudimentary short flagellum 0.3 μm long and hook-shaped, 3) long flagellum with small spherical knob-scales, 4) absence of pyrenoid and stigma, and 5) marine habitat. This new species andP. salina seem to be most closely related to each other but their colour, the insertion of their three appendages, the shape of their knob scales, and their cell periplasts are quite different from one another. Contribution No. 1879 from the Institute of Oceanology, Academia Sinica  相似文献   

Observations of High Resolution Spectra of Comet Hale-Bopp (C/1995 O1) at the SAO of the RAS     

Churyumov  K. I.  Klesachonok  V. V.  Mussaev  F. A.  Bikmaev  I. F.  Galazutdinov  G. H. 《Earth, Moon, and Planets》1997,78(1-3):105-110

We present the results of the preliminary study of the comet Hale-Bopp spectrum obtained April 17, 1997 by K. Churyumov and F. Mussayev with the help of the 1-meter Zeiss reflector and the echelle spectrometer (spectral resolutionλ/Δ λ ≈ 50000), CCD and the long slit, oriented along the radius-vector(“Sun-comet direction”). Energy distributions for three selected regions including the C₃, C₂ (0-0) and CN(Δ ν = 0) molecules emissions of the comet Hale-Bopp spectrum were built. The rotational lines of the CN(Δ ν = 0) band were identified. The nature of the high emission peak near λ 4020 Å in the C₃ band is discussed. The presence of the cometary continuum of the nonsolar origin is assumed.  相似文献   

烟台港和邻近锚地及其入境船舶压舱水中的浮游植物   总被引：8，自引：0，他引：8  

李伟才  孙军  宋书群  雷质文  贾俊涛  王丹 《海洋湖沼通报》2006,(4):70-77

本文根据2003年5月到2004年4月间烟台港和邻近锚地水域及其船舶压舱水中的浮游植物调查资料,对浮游植物的群落结构和压舱水对其影响进行了初步研究。结果表明,烟台港和临近锚地的浮游植物群落主要由温带沿岸性物种组成,以硅藻为主,还有少量甲藻和金藻;对比船舶压舱水中浮游植物群落发现:压舱水中浮游植物群落和本地群落的结构有很大差异,有害物种在压舱水中出现的频率较高。  相似文献   

近江牡蛎作为重金属污染生物指示种的初步研究   总被引：17，自引：2，他引：17  

陆超华 《台湾海峡》1994,13(1):14-20

对近江牡砺作为监测广东岸海域Ｃｕ，Ｚｎ，Ｃｄ，Ｐｂ污染的生物指示种的可能性进行了初步研究和评价，应用ＰＯＬＹＤＩＶ统计技术对采自广东沿岩海域１４个站点的近江牡盛的重金属含量进行了统计分析，结果表明近江牡蛎受Ｃｕ，Ｚｎ，Ｃｄ污染的地理分布类型与广东沿海排放的工业废水的分布以及潮间带生物和表层沉积物的Ｃｕ，Ｚｎ，Ｃｄ含量分布相一致，因此，近江牡蛎可作为监测研究Ｃｕ，Ｚｎ，Ｃｄ污染的可靠生物指示种，但能  相似文献   

胶州湾海水微表层粘度及其在海-气通量计算中的作用     

张安慧  张正斌  刘莲生  林彩 《中国海洋大学学报(自然科学版)》2002,32(3):456-462

物质海 -气通量计算新建议中将物质海 -气通量计算公式 F=K(CL- b Cg)中的 CL 用CL ( SML ) 代替。本文着重于对公式中质量迁移系数 K的讨论。在测定了海水微表层、次表层水粘度并同时测定了其它一些化学参量基础上 ,得出如下结论 :海水粘度与盐度、碱度有一定相关性 ;微表层与次表层海水的粘度变化小于 3%。因此 ,海水微表层效应影响 K值 ,与海水微表层效应影响物质浓度相比 ,可以不考虑。  相似文献   

Long-Term Investigation of Spatio-Temporal Variations in Faunal Composition and Species Richness of Megabenthos in Ise Bay,Central Japan     

Monthon?Ganmanee  Teruyoshi?Narita  Hideo?SekiguchiEmail author 《Journal of Oceanography》2004,60(6):1071-1083

Based on our long-term data from megabenthos sampling from 1993 to 2002 in Ise Bay, central Japan, we examined spatio-temporal variations in taxon composition, species richness and its distribution of megabenthos in the bay in relation to the occurrence of the oxygen-poor water (i.e. oxygen content less than 3 ppm) in bottom waters of the bay. A total of 261 species were identified including 6 cnidarians, 1 tentaculate, 5 annelids, 71 molluscs, 72 crustaceans, 16 echinoderms, 12 urochordates and 78 pisces. Of the most abundant 10 megabenthos species, the following 4 species of echinoderms made up more than the half of megabenthos biomass: Luidia quinaria, Echinocardium cordatum, Asterias amurensis and Astropecten scoparius. Species richness of megabenthos varied significantly between seasons and among stations. The severity and period of occurrence of the oxygen-poor water developing every summer play an important role in determining spatial distributions of species richness in the bay.  相似文献   

Predation by herring (Clupea harengus) and sprat (Sprattus sprattus) on Cercopagis pengoi in a western Baltic Sea bay     

Gorokhova  Elena; Fagerberg  Towe; Hansson  Sture 《ICES Journal of Marine Science》2004,61(6):959-965

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