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采用硫酸铵分部沉淀与凝胶过滤的方法,进行藓羽藻Rubisco的分离研究。结果表明,分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带,分别为Rubisco大亚基与小亚基;与菠菜相比,藓羽藻Rubisco大亚基分子量与菠菜基本相同,而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明,Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多,分离后活力有所上升,但仍比粗提液活力弱;在Rubisco活力测定过程中,藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同,在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。 相似文献
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某港口堆场地基上部5.0m系吹填而成,地表下18m范围均属软土,经真空预压初级加固后地基承载力仅在80—90kPa,局部区域上部淤泥土层土性指标较差,含水量大于50%,地基承载力不足60kPa。为了使地基达到230kPa的承载要求,设计采用深层搅拌法加固超软弱地基。通过现场成桩工艺试验和检测表明,桩身水泥土强度在90d龄期时大于1.80MPa,单桩承载力标准值大于150kN,以φ600桩径、桩长13.5m、置换率为0.308和φ500桩径、桩长13.5m、置换率为0.267两种方案布置的复合地基承载力标准值均超过了230kPa的设计要求。试验结果表明,深层搅拌法在港口超软弱地基土应用只要施工工艺适当,完全可以使地基承载力提高2—3.5倍以土,从而节省大量的工程投资。 相似文献
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Patterns and controls of surface sediment distribution: west-central Florida inner shelf 总被引:2,自引:0,他引:2
Gregg R. Brooks Larry J. Doyle Richard A. Davis Nancy T. DeWitt Beau C. Suthard 《Marine Geology》2003,200(1-4):307-324
The west-central Florida inner shelf represents a transition between the quartz-dominated barrier-island system and the carbonate-dominated mid-outer shelf. Surface sediments exhibit a complex distribution pattern that can be attributed to multiple sediment sources and the ineffectiveness of physical processes for large-scale sediment redistribution. The west Florida shelf is the submerged extension of the Florida carbonate platform, consisting of a limestone karst surface veneered with a thin unconsolidated sediment cover. A total of 498 surface sediment samples were collected on the inner shelf and analyzed for texture and composition. Results show that sediment consists of a combination of fine quartz sand and coarse, biogenic carbonate sand and gravel, with variable but subordinate amounts of black, phosphorite-rich sand. The carbonate component consists primarily of molluskan fragments. The distribution is patchy and discontinuous with no discernible pattern, and the transition between sediment types is generally abrupt. Quartz-rich sediment dominates the inner 15 km north of the entrance into Tampa Bay, but south of the Bay is common only along the inner 3 km. Elsewhere, carbonate-rich sediment is the predominate sediment type, except where there is little sediment cover, in which cases black, phosphorite-rich sand dominates. Sediment sources are likely within, or around the periphery of the basin. Fine quartz sand is likely reworked from coastal units deposited during Pleistocene sea-level high stands. Carbonate sand and gravel is produced by marine organisms within the depositional basin. The black, phosphorite-rich sand likely originates from the bioerosion and reworking of the underlying strata that irregularly crop out within the study area. The distribution pattern contains elements of both storm- and tide-dominated siliciclastic shelves, but it is dictated primarily by the sediment source, similar to some carbonate systems. Other systems with similar sediment attributes include cool-water carbonate, sediment-starved, and mixed carbonate/siliciclastic systems. This study suggests a possible genetic link among the three systems. 相似文献
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This study uses a comparative approach to examine responses of marine ecosystems to climatic regime shifts. The three seas surrounding the Korean peninsula, the Japan/East Sea, the East China Sea and the Yellow Sea represent three contiguous but distinct ecosystems. Sampling has been carried out by the National Fisheries Research and Development Institute of South Korea since 1965, using the same methods in all three seas. Sampling was generally synoptic. Amplitude time series of 1st EOF modes for temperature, salinity, zooplankton biomass and concentrations of four major zooplankton taxa were used to determine whether the three marine ecosystems respond in a similar manner to climate variations. Temporal patterns of the variables were strongly similar among the three seas at decadal time scales, but very weakly similar at interannual scales. All three seas responded to a climatic regime shift that occurred in 1989. Temperature, zooplankton biomass and copepod concentrations increased in the late 1980s or early 1990s in all three seas. Concentrations of amphipods, chaetognaths and euphausiids also increased in the Japan/East Sea and the East China Sea, but not the Yellow Sea. The Yellow Sea ecosystem differs strongly from the other two seas, and water exchange between the Yellow Sea and the East China Sea is much weaker than that between the East China Sea and Japan/East Sea. Spatial patterns of zooplankton determined by the EOF analysis were closely related to currents and fronts in each of the three seas. 相似文献
109.
渤海大型底栖动物次级生产力的初步研究 总被引:12,自引:2,他引:12
1998年 9月和 1999年 4月分别对渤海 2 0个站进行了大型底栖动物两个航次的调查取样。整个研究海域大型底栖动物年次级生产力平均值为 6 .4 9g(AFDW) /(m2 · a) ,其中渤海海峡以东海域较高 ,为 12 .5 9g(AFDW) /(m2· a) ;渤海中部为 4 .4 6 g(AFDW) /(m2· a) ,渤海大型底栖动物年平均 P/B为 0 .82 相似文献
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