Effects of waterborne Cu and Cd on anti-oxidative response,lipid peroxidation and heavy metals accumulation in abalone Haliotis discus hannai ino     

Lei  Yanju  Zhang  Wenbing  Xu  Wei  Zhang  Yanjiao  Zhou  Huihui  Mai  Kangsen 《中国海洋大学学报(英文版)》2015,14(3):511-521

The aim of this study was to compare the effects of waterborne copper(Cu) and cadmium(Cd) on survival, anti-oxidative response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals(initial weight: 7.49 g ± 0.01 g) were exposed to graded concentrations of waterborne Cu(0.02, 0.04, 0.06, 0.08 mg L-1) or Cd(0.025, 0.05, 0.25, 0.5mg L-1) for 28 days, respectively. Activities of the anti-oxidative enzymes(catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST), contents of the reduced glutathione(GSH) and malondiadehyde(MDA) in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mg L-1 Cu caused 100% death of abalone on the 21 st, 10 th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopancreas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense against Cu or Cd. In conclusion, anti-oxidative mechanism in abalone to resist waterborne Cu did not follow the same pattern as that for waterborne Cd.  相似文献   

Preliminary observations of the internal tide over the shelf west of New Zealand   总被引：1，自引：1，他引：0  

B. R. Stanton 《新西兰海洋与淡水研究杂志》2013,47(4):703-712

Abstract

Semidiurnal variations in the depth of the thermocline observed near the shelf edge north‐west of Cape Egmont are probably caused bv an internal tide generated at around 200 m depth over the continental slope. The observations suggest that in this region an internal tide, with amplitude of about 20 m, propagates onto the shelf with a speed of approximately 0.5 m·s^?1 and a wavelength of about 22 km.  相似文献   

饲料中高水平铜对斜带石斑鱼(Epinephelus coioides)生长和铜、铁、锰、锌含量的影响   总被引：1，自引：0，他引：1  

叶超霞  刘永坚  田丽霞  阳会军  王安利  周立斌 《海洋与湖沼》2013,44(3):606-610

采用铜适量(4.47mg/kg)和铜过量(1127.51mg/kg)的两种饲料投喂初始体重为(12.9±0.2)g的斜带石斑鱼(Epinephelus coioides)8周,研究饲料中过量铜对斜带石斑鱼生长性能、形态学指标、体组成和鱼体微量元素含量的影响。结果表明,当饲料铜含量由4.47mg/kg升高到1127.51mg/kg时,斜带石斑鱼增重率由228%下降到63%,饲料效率由1.12下降到0.51,肠脂比由2.90%下降到1.69%。全鱼和肝脏中的铜含量均随饲料铜水平的增加而显著增加,斜带石斑鱼全鱼铜含量由2.98mg/kg上升到34.98mg/kg,肝脏铜含量由36.6mg/kg上升到1364.0mg/kg。但饲料中过量铜对斜带石斑鱼的存活率、鱼体肥满度、脏体比、肝体比和全鱼体组成没有显著性影响。摄食高铜饲料的斜带石斑鱼肝脏锌含量显著升高,锌可能在铜的解毒过程中起着重要的作用。  相似文献   

Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea     

Kinder A  Sierts-Herrmann A  Biselli S  Heinzel N  Hühnerfuss H  Kammann U  Reineke N  Theobald N  Steinhart H 《Marine environmental research》2007,63(5):506-515

Heat shock proteins 70 (hsp70) are known to be induced by a great variety of chemical stressors. The effects of different environmental contaminants, which were identified in sediments of the North Sea and the Baltic Sea as main contaminants or as contaminants of special toxicological interest, on the extent of expression of hsp70 in a permanent cell line Epithelioma papulosum cyprini (EPC) were investigated. Concentrations of the single contaminants leading to elevated hsp70 levels were compared to the respective concentrations of these contaminants encountered in the sediment extracts thus allowing an assessment of their contribution to the toxicity of these sediments. Analysis of hsp70 was performed using western blotting techniques with subsequent comparative quantification by densitometry. Eleven of thirteen contaminants have shown a dose-dependent increase in hsp70 levels. An expression of hsp70 was observed in most cases between 0.1 and 10 microg/mL test solution. Some of the investigated stressors led to extremely high hsp70 contents even at low concentrations. However, in most cases contaminant concentrations in sediments were too low to cause an effect. For this reason, effects of the tested sediments are attributed to other contaminants or rather to mixtures of stressors.  相似文献   

渤中25-1油田沙河街组低孔低渗储层特征及分类评价   总被引：3，自引：0，他引：3  

杨玉卿  潘福熙  田洪  徐晋  陈力群 《现代地质》2010,24(4)

渤中25-1油田位于渤海南部海域,其主力油层为古近系沙河街组,总体上属于低孔低渗储层。通过薄片、扫描电镜、X衍射分析和测井解释等手段,对沙河街组砂岩储层的岩石学特征、沉积特征、物性和孔隙结构特征进行了分析研究。沙河街组沙三段为较深水湖泊环境下的浊积扇沉积,沙二段为扇三角洲沉积;岩性主要为岩屑长石砂岩,储层孔隙类型为混合孔隙、次生孔隙和原生孔隙,由于沙三段溶蚀孔隙发育较沙二段少,沙三段物性较沙二段差。结合储层试油结果,提出了低孔低渗储层宏观成因、微观孔隙结构和综合评价3种分类方案,总体上把储层分为好、中和差3类,对应产能分别为大于80 m3/d、介于10~100 m3/d之间、小于10 m3/d和微量或无产能。经过该油田沙河街组5口井、15个储层段的测试验证,储层宏观成因分类评价结果与实际试油结果符合率达到86.67%,微观孔隙结构分类和综合分类评价结果与实际试油结果基本吻合。  相似文献   

大唐国际东营一期49.5 MW风电场工程桩基施工及其原体试验          下载免费PDF全文

李吉林  张旭虎 《探矿工程》2010,37(9):40-45

大唐国际东营风电一期工程拟建风电场场区位于东营市东部沿海区域,拟建场地上覆第四系地层,结构松散,厚度较大,工程性质较差,地基强度较低,不能满足重要建（构）筑物对其强度及变形的要求,且场地内的饱和粉土及砂土在地震影响烈度达Ⅶ度时将可能产生液化现象,因此需进行地基处理。根据现场岩土条件,拟采用预应力高强混凝土管桩（PHC）,需进行桩基原体试验。主要介绍了桩基原体试验施工过程、检测方法及试验成果等。  相似文献   

海洋微塑料对重金属的吸附行为及其复合毒性研究进展     

张晨曦  孙景春  林承刚 《海洋科学》2022,46(8):155-170

微塑料在海洋中的污染情况已经受到了人们广泛关注,但其与重金属相互作用产生的潜在生态风险依然需要进一步研究。本文主要综述了海洋中微塑料的来源及海水、沉积物和生物体内微塑料的污染现状,总结了部分海域微塑料上的重金属富集特征,并介绍了部分微塑料对重金属的吸附模型,最后总结分析了微塑料单独及与重金属协同作用对海洋生物的毒性效应。微塑料与重金属相互作用的结果依然存在许多不确定性,对生物体产生的毒性效应是协同、拮抗还是其他交互作用仍需更多的实验研究。本文旨在为评估微塑料与重金属相互作用造成的生态风险提供支撑,并为今后相关研究的开展提供参考。  相似文献   

内蒙古金厂沟梁金矿床成矿流体特征及稳定同位素研究   总被引：1，自引：0，他引：1  

孙珍军  李学源  于赫楠  孙国胜  朱继浩  陆丽娜  薛怀宇  苏蓉 《世界地质(英文版)》2014,(3)

对金厂沟梁金矿床含金石英脉中流体包裹体进行了岩相学、显微测温、单个包裹体的激光拉曼测试以及O和S同位素组成等方面研究。结果表明,成矿流体气相成分主要为H2 O,属H2 O-NaCl体系,包裹体均一温度为148.7℃～352℃,盐度[ w （ NaCl ）/％]为1.05％～5.99％。δ18 O值为-1.1‰～3.0‰,说明成矿流体继承了变质流体的特征,仍以岩浆水和后期大气降水为主;δ34 SV-CDT组成为0.6‰～4.3‰,平均值0.817‰,极差为3.1‰,表明成矿流体中的硫主要来自于幔源,其次为围岩。综合分析认为,金厂沟梁金矿床是在燕山晚期太平洋板块俯冲导致岩石圈的快速减薄、拆沉的拉张环境下形成的浅成造山型金矿。  相似文献   

一种程控宽频带多波形信号发生器的研制     

胡仕兵  赵敏智王　波 《成都信息工程学院学报》2014,(1):33-38

针对目前专门的函数信号发生器IC功能简单、精度不高、调节方式不灵活等缺点和DDS芯片外围电路设计复杂、成本较高等问题,设计研制了一种程控宽频带多波形信号发生器。该信号发生器采用单片机STC89C52控制专用函数发生器MAX038实现,通过键盘设置输出波形类型、频率、占空比和幅度等各种参数,采用闭环控制方法对输出频率自动反馈控制以提高频率精度,并由八位LED显示器实时显示状态信息。实际运行结果表明:该系统可以产生频率范围在1Hz~20MHz、幅度在2~10V(VPP)连续可调、占空比在10%~90%变化的三角波、锯齿波、方波、正弦波和PWM波,输出频率精度小于0.01%。该仪器具有电路结构简单、性能优良、成本低廉、功能多样、使用方便等优点,在生产实践和科技研究领域中具有广泛的应用前景。  相似文献   

分段多项式拟合在动三轴试验数据处理中的应用     

周松望  周杨锐  吴海京  戴少军  沈志明  董明明 《海洋学报》2014,36(5):186-189

动三轴采集的试验数据点(剪应变与动剪模量和阻尼比之间)具有离散性和有限性,为方便应用需要对数据点进行拟合,但现有经验公式不一定很好吻合试验数据。为得到不同剪应变与对应的动剪模量和阻尼比之间的关系,本文利用最小二乘原理,对渤海某海洋平台地基土进行动三轴试验获得的动剪模量、阻尼比与剪切应变的数据进行了分段多项式的拟合。数据处理过程是先对剪应变值进行了压缩处理,然后再分段低次多项式拟合,得到多项式拟合参数。通过此项改进解决了正规方程解中的病态问题,且拟合效果良好。  相似文献