Nucleotide sequence of β-tubulin gene from the soft coralscleronephthya gracillimum (kükenthal)     

Seungshic Yum  Seonock Woo  Man Chang  Taek-Kyun Lee  Jun-Im Song 《Ocean Science Journal》2005,40(1):55-59

We cloned the complete cDNA of the β-tubulin from the soft coral,Scleronephthya gracillimum (Kükenthal) (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5′-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of theS. gracillimum β-tubulin comprised 1541 bp, not including the poly (A)⁺ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16-402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the β-tubulin gene inS. gracillimum.  相似文献   

三角褐指藻磷酸甘油酸变位酶基因可能侧翼序列的筛选、克隆以及序列测定   总被引：2，自引：2，他引：2  

王广策  孙海宝  曾呈奎 《海洋与湖沼》2002,33(3):259-264

采用RT PCR的方法从酵母中成功地得到了磷酸甘油酸变位酶的cDNA基因 ,分别用32 P和地高辛 ddUTP标记以用作探针。以32 P标记的探针筛选三角褐指藻基因组文库 ,获得了 4kb的阳性DNA片段 ;进一步分析发现 ,该 4kb片段的真正阳性区域是位于片段端部的30 6bp的序列 ,因此认为该序列为三角褐指藻磷酸甘油酸变位酶基因的侧翼部分。克隆该30 6bp的DNA片段 ,并且测定其序列。结果表明 ,该 30 6bp的DNA片段包含两个同向重复序列 ,每个重复序列的大小为 1 1 6bp ,在每个重复序列中均含有GGTTCAATGT区域 ,这与一般常见的真核基因 5′端的CAATbox有相似之处。  相似文献   

Using global genome approaches to address problems in cod mariculture     

Goetz  Frederick W.; McCauley  Linda; Goetz  Giles W.; Norberg  Birgitta 《ICES Journal of Marine Science》2006,63(2):393-399

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雨生红球藻cDNA表达文库的构建与初步分析   总被引：1，自引：0，他引：1  

梁成伟  李友训  孟春晓  赵方庆  杨庆利  秦松 《海洋通报》2006,25(2):93-96

为了筛选雨生红球藻虾青素合成过程中的关键酶基因的反式调节因子基因,以雨生红球藻的绿细胞为材料,提取了高质量的总RNA,并分离纯化了mRNA,经过反转录后的得到cDNA,构建了以λ-ZAPExpress为载体的表达型cDNA文库。经检测,所构建的文库的滴度为5.1×105,重组率为100%。用PCR方法对文库的质量进行了鉴定,文库的平均插入片断的大小为1.7kb。雨生红球藻cDNA表达文库的构建为研究虾青素的代谢工程奠定了基础。  相似文献   

五氯苯酚暴露致花鲈(Lateolabrax japonicus)肝脏损伤的转录组分析     

郑丹  焦海峰  严小军  张乐  阮松林 《海洋与湖沼》2018,49(2):352-360

为了探明五氯苯酚暴露后花鲈(Lateolabrax japonicus)肝脏的损伤变化,本文利用Illumina Hi SeqTM2000测序技术对不同浓度五氯苯酚(0,0.1,1.0,10.0,100μg/L)暴露后花鲈肝脏组织进行了转录组测序。经Trinity软件拼接,得到clean reads共247914284条,获得53716条基因。对照不同浓度暴露组和对照组的转录组测序结果,获得共有的差异性表达基因135个,特异性的差异基因数分别有127—819个不等,差异蛋白表达数量随着受试浓度的升高而上升。经BLAST搜索,其中有21459、26464、18896、12403、17262和19159条分别注释到Swiss-prot、Nr、Pfam、KEGG、KOG和GO数据库。KEGG代谢通路分析结果显示获得的差异基因分别映射到6大类141条通路,这些差异表达基因在与环境信息进程相关的信号传导和与组织系统相关的免疫系统的通路中富集最多。KOG数据库预测和分类表明16571条基因归属于25大类,参与一般功能预测和信号传导机制的数量最多,参与翻译后修饰、蛋白质转换与分子伴侣路径的也占较大比例。总体来看,在受到五氯苯酚类物质胁迫后,花鲈体内的信号传导系统首先发生响应,导致机体出现紊乱。污染物暴露后,花鲈机体的应对机制尚待深入分析。  相似文献   

Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene     

Wen Zhang  Lin-sheng Song  Jang-Seu Ki  Chun-Kwan Lau  Xiang-Dong Li  Pei-Yuan Qian 《Estuarine, Coastal and Shelf Science》2008

Sulfate-reducing bacteria (SRB) are important regulators of a variety of processes in coastal marine sediments regarding organic matter turnover, biodegradation of pollutants, and sulfur and carbon cycles. Yet their community compositions have not been investigated in polluted harbor sediments. This study described the diversity and spatial variation of SRB communities in surface sediments in Victoria Harbor, Hong Kong. The spatial variation of SRB communities was described by terminal restriction fragment length polymorphism (T-RFLP). The results showed that the most diversified terminal restriction fragments were found at polluted sites. In addition, cluster analysis indicated that although the SRB communities were different at the two polluted sites, they were still more similar to each other than to the two more distant reference sites. Based on a dsrAB clone library constructed at a polluted site, diversified SRB were found, represented by 30 Operational Taxonomic Units (OTUs). Upon comparisons among the SRB sequences detected from this study and those in the GenBank, five clades of SRB were found. Three clades belonged to the known families Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteriaceae. The majority of sequenced clones, which distantly related to sequences in the GenBank, constituted the remaining two unclassified groups, suggesting unique SRB members related to the polluted harbor environment. Statistical analyses indicated that estimated SRB richness correlated with environment factors such as sulfur content, acid volatile sulfate, and redox potential.  相似文献   

知识经济与图书情报工作     

戴曦  纪驿桥 《成都信息工程学院学报》2000,15(2):186-189

知识经济是近年来世界范围内兴起的一个新概念。阐述了知识经济时代图情工作面临的机遇和挑战 ,同时阐述了图情工作在知识经济发展中的地位和作用。  相似文献   

鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)髓样分化因子88基因表达的影响     

高晶  任毅鹏  潘宝平  闫春财 《海洋与湖沼》2015,46(2):440-445

经转录组测序后筛选并克隆得到青蛤(Cyclina sinensis)髓样分化因子88(myeloid differenttiation factor 88,My D88)的c DNA序列。在鳗弧菌(Vibrio anguillarum)胁迫下,采用荧光定量PCR法分析了My D88基因在青蛤体内的表达过程。结果显示,青蛤My D88基因的开放阅读框为1521bp,编码506个氨基酸,分子量约为57.14k Da,氨基酸N段存在DEATH结构域,C段存在TIR结构域(Toll/Interleukin-1 receptor domain)。My D88基因在青蛤血淋巴、肝脏、外套膜、鳃和闭壳肌等组织中普遍表达,但在血淋巴中表达量最高,与其它组织出现显著性差异(P0.05)。通过检测鳗弧菌刺激下My D88基因在青蛤血淋巴中的表达值,发现My D88基因在24h开始升高,48h达到最大值,约为对照组的10倍,实验组与对照组及空白组均出现了极显著性差异(P0.01);研究结果表明,该基因在软体动物的免疫应答反应中对革兰氏阴性菌有识别作用。  相似文献   

Comparative analysis on transcriptome sequencings of six Sargassum species in China     

WANG Guoliang  SUN Jing  LIU Guiming  WANG Liang  YU Jun  LIU Tao  CHI Shan  LIU Cui  GUO Haiyan  WANG Xumin  WU Shuangxiu 《海洋学报(英文版)》2014,33(2):37-44

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对虾白斑综合症病毒极早期基因启动子筛选文库的构建     

林梵宇  徐丽美  杨丰 《台湾海峡》2010,29(2):184-188

对虾白斑综合症病毒（white spot syndrome virus,WSSV）是危害对虾养殖业的主要病原,而WSSV极早期基因对病毒功能基因的转录翻译和调控起着至关重要的作用.本研究将WSSV基因组以超声法随机断裂至400～900bp DNA片段,并插入启动子缺失且多克隆位点下游具有报告基因的载体来构建文库,转染昆虫细胞Hi5.倒置荧光显微镜观察发现部分转染后的细胞有增强型绿色荧光蛋白（EGFP）的表达,表明这部分细胞中的DNA片段可能具有极早期启动子活性.流式细胞仪分析显示,这部分荧光细胞占总细胞的比例为0.35%.由于转染效率为50%,可认为占比0.7%的WSSV基因组随机片段能在Hi5细胞中激发下游报告基因的转录翻译,可能具有极早期基因启动子活性.这对于进一步筛选和鉴定WSSV极早期基因及开展极早期启动子研究具有重要的意义.  相似文献