环境因子对三种常见微藻细胞中二甲基硫丙酸含量影响的初步研究   总被引：2，自引：0，他引：2  

李炜  焦念志 《海洋与湖沼》1999,30(6):635-639

以3种常见的海洋浮游藻类──扁藻、杜氏藻和牟氏角刺藻作为实验材料,采用正交方法设计实验条件,研究盐度、温度和光强的变化对藻类细胞内二甲基硫丙酸（DMSP）含量的影响。结果表明,3种藻类细胞DMP含量相差很大。扁藻DMSP含量最高,其次为杜氏藻,牟氏角刺藻含量最低。种间差别的影响明显高于环境条件的变化对藻细胞DMSP含量的影响。3种环境因子对藻细胞DMSP含量的影响效果不同,盐度变化引起藻细胞DMP含量的变化最为显著,其次为温度和光强。随着盐度的增加,扁藻、杜氏藻和牟氏角刺藻细胞内DMSP含量均呈上升趋势。  相似文献   

Cascades of sub-decadal,channel-floodplain changes in low-gradient,non-vegetated reaches near a dryland river terminus: Salar de Uyuni,Bolivia     

Jiaguang Li  Stephen Tooth  Guangqing Yao 《地球表面变化过程与地形》2019,44(2):490-506

The terminus of the ephemeral Río Colorado is located at the margins of Salar de Uyuni, Bolivia, the world's largest salt lake. The low-gradient (<0.0006 m m^-1), non-vegetated reaches approaching the terminus provide an excellent natural laboratory for investigating cascades of channel-floodplain changes that occur in response to quasi-regular flows (at least once annually) and fine-grained sediment supply (dominantly silt and clay). High-resolution satellite imagery (<0.65 m, various dates from 2004 onwards) and field data reveal widespread, pronounced and rapid morphodynamics on sub-decadal timescales, including channel erosion and chute cutoff formation, and development of crevasse channels and splays, floodouts (unchannelled surfaces at channel termini), and erosion cells (floodplain scour-transport-fill features). In particular, following high annual precipitation (>400 mm) in 2004–2005 and two subsequent high magnitude daily precipitation events (~40 mm), all of which led to widespread flooding, numerous crevasse splays formed between 2004 and 2016, avulsions occurred at nearby floodouts, and erosion cells downstream of the splays and floodouts underwent striking morphological changes. High-precision GPS data reveal two preferential localities for erosion cell development: partially or fully abandoned channels with crevasse splay remnants, and topographic lows between channels. In this overall low-gradient setting, comparatively high gradients (up to ~0.0006 m m^-1) at the edge of splay deposits and topography created by crevasses and abandoned channels may initiate knickpoint retreat and thereafter erosion cell development. Abandoned channels with splays tend to give rise to narrow, deep erosion cells, while topographic lows promote relatively shallow, wide erosion cells. In both situations, erosion cells may extend upslope and downslope, and eventually connect to form straight channels. The channel-floodplain morphodynamics near the Río Colorado terminus extend previous analyses of low-gradient, dryland river systems, particularly because the lack of vegetation and quasi-regular floods drive cascades of rapid changes on sub-decadal timescales. © 2018 John Wiley & Sons, Ltd.  相似文献   

Feeding,defaecation and absorption efficiency in the Cape rock lobster jasus lalandii     

《African Journal of Marine Science》2013,35(1):59-65

In order to estimate the absorption rate of carbon by the palinurid lobster Jasus lalandii, the net quantity of ingested carbon and gross carbon content of faeces must be known. Experimental animals were selected for consistent feeding and subsequently starved for 72 h. Ingested carbon was established by determining the carbon content of the prey mussel Choromytilus meridionalis under controlled aquarium conditions through five eating/defaecating cycles. The losses of particulate and dissolved organic carbon due to messy feeding were measured and amounted to 20,1 per cent of the food presented. Of the net carbon ingested, 20,5 per cent was voided as faeces, thereby giving a mean absorption efficiency of 79,5 per cent (n = 15, SD = 4,6%). J. lalandii, as a top carnivore in the inshore Benguela ecosystem, is therefore highly efficient in the utilization of ingested carbon. In animals of commercial size (≥ 89 mm carapace length), small variations in absorption efficiencies exhibited were neither dependent on body mass nor ingested mass, but may rather be attributed to scatter inherent in all biological systems.  相似文献   

日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养   总被引：1，自引：0，他引：1  

逄越  李庆伟 《海洋科学》2012,36(1):23-29

以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。  相似文献   

免疫细胞化学法测定鱼类淋巴囊肿病毒(LCDV)滴度     

宋佳蕾  绳秀珍  战文斌 《中国海洋大学学报(自然科学版)》2012,42(5):55-59

利用免疫细胞化学法测定鱼类淋巴囊肿病毒(LCDV)滴度.以牙鲆鳃细胞系(FG)作为感染细胞,将生长旺盛的FG细胞接种于48孔培养板中培养至形成细胞单层,用2倍连续稀释的LCDV粗提液分别接种FG细胞.固定各稀释度LCDV感染后的FG细胞,孵育抗牙鲆LCDV单克隆抗体,其后再运用生物素-亲合素反应系统,以碱性磷酸酶底物APRed试剂盒发色.倒置显微镜观察,被病毒感染的FG细胞的细胞质呈现红色,未被感染细胞的细胞质呈无色.记录各稀释度病毒感染的阳性细胞孔数,按Reed-Muench法计算组织细胞培养半数感染量(TCID0).结果显示,免疫细胞化学法测得LCDV在FG的滴度为1.77×210 TCID50/mL.该法可以用来有效测定LCDV滴度,且结果直观、准确性较好,灵敏度较高.  相似文献   

Effectiveness of grass strips in trapping suspended sediments from runoff     

Chengzhong Pan  Lan Ma  Zhouping Shangguan 《地球表面变化过程与地形》2010,35(9):1006-1013

Little information is available concerning the performance of grass strips for erosion control from steep cropland. An experiment was conducted on 5‐m‐long grass strips with slopes of 3°～15° that were subjected to silt laden runoff and simulated rainfall, to investigate the sediment trapping processes. The grass strips had three treatments including intact grass control (C), no litter (dead grass material covering the soil surface was removed) (NL), and no litter or leaves (only 2～3 cm grass stems and roots were reserved) (NLL). Generally the grass strips had a high effectiveness in trapping sediment from steep cropland runoff. Sediment trapping efficiency (STE) decreased with increasing slope gradient, and even for a 15° slope, STE was still more than 40%. Most sediment deposited in the backwater region before each grass strips. The removal of grass litter or/and leaves had no significant influence on STE. The sediment median size (D₅₀) in inflow was greater than that in outflow, and the difference (ΔD₅₀) decreased with increasing slope. A positive power relationship between STE and ΔD₅₀ can be obtained. Grass strips were more effective in trapping sediments coarser than 10 or 25 µm, but sediments finer than 1 µm were more readily removed from runoff than particles in the range of 2 to approximately 10 µm. Grass litter had less influence on flow velocity than leaves because the deposited sediment partially covered the litter layer. Mean flow velocity and its standard deviation were negatively correlated with STE, and they can help make good estimation of STE. Results from this study should be useful in planting and managing forage grass to effectively conserve soil loss by runoff from steep slopes on the Loess Plateau of China. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

一种基于交叉剖面的地质模型构建方法     

陈国良  刘修国  盛谦  张勇慧 《岩土力学》2011,32(8):2409-2415

针对基于近似平行断面数据的建模方法的缺点,利用交叉地质剖面数据,提出了一种通过划分空间单元格的思路来构建地质结构模型的方法。该方法基于“分而自治”的思想,将复杂的整体的建模区域划分为单个单元格内的建模,待每个格子内的建模完成之后,采用相反的思路,将所有格子内的模型拼接起来形成最终的建模区域内的整体模型  相似文献   

驼背鲈外周血细胞的形态学研究   总被引：1，自引：0，他引：1       下载免费PDF全文

王静香  李加儿  区又君 《海洋科学》2011,35(4):77-82

利用Wright's和Giemsa双重染色法和改良的血涂片染色法两种方式,通过光镜对驼背鲈(Cromileptes altivelis)的血涂片进行了观察.结果表明:驼背鲈的血涂片中可区分出6种细胞--红细胞、嗜中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞、血栓细胞,偶尔可见到分裂的红细胞、分裂的血栓细胞、核影红细胞和...  相似文献   

应用纤维素示踪北京市PM_2.5天然植被排放来源的研究     

丁毅  张烃  董树屏  刘咸德  梁汉东 《岩矿测试》2013,32(5):738-746

大气细粒子(PM_2.5)污染是全球尤其是我国许多城市的重要环境问题。利用示踪物质识别和估算大气颗粒物来源是公认的可靠技术,纤维素可以作为示踪物质表征一次颗粒物的天然植被排放来源。本文针对大气细粒子中纤维素含量低、全程序空白相对较高等难点,优化建立了纤维素酶水解、GOD-苯酚-四氨基安替比林测糖法,在我国首次应用于测定大气PM_2.5中纤维素的含量,估算天然植被排放源的贡献。方法检出限为0.26 μg/m³(纤维素),可以满足大气PM_2.5纤维素测定要求;而且有效地降低了空白,全程序空白值(36.5 μg葡萄糖)低于文献方法空白值(53.8 μg葡萄糖),使之更适合于PM_2.5的测定。使用本方法对2012年5月至6月采集的北京市大气PM_2.5样品进行分析,纤维素检出率为96%,纤维素的平均含量为(0.573±0.17) μg/m³,折合为天然植被排放量占PM_2.5质量浓度的1.37%±0.65%;天然植被排放源对有机碳的平均贡献率为4.4%,最大达到9.2%,反映出天然植被排放是北京市PM_2.5的重要来源之 一。本研究方法为我国城市大气颗粒物(包括总悬浮颗粒物、PM₁₀、PM_2.5等)来源识别提供了新的手段。  相似文献   

Biofilm Formation by Microorganisms Isolated from Paper Mill Effluent and its Public Health Implications     

Priyanka Lal  Vishnu Agarwal  Parul Pruthi  Swaranjit Singh Cameotra  Vikas Pruthi 《洁净——土壤、空气、水》2008,36(12):963-968

The microbiological assessment of biofilm formation from paper mill effluent discharged through a pipeline revealed a maximum microbial count for Pseudomonas (5·10⁶ cfu/mL) followed by Staphylococcus (4·10⁶ cfu/mL), Bacillus (8.2·10⁵ cfu/mL), Burkholderia (7.2·10⁵ cfu/mL), Enterobacter (5.3·10⁴ cfu/mL), Acinetobacter (4.1·10³ cfu/mL), Alcaligenes (1.2·10² cfu/mL) and Klebsiella (0.8·10² cfu/mL) species. Among these species, the maximum biofilm formation was observed after 24 h of incubation by Pseudomonas sp. using a crystal violet (CV) assay. This isolate was later identified by 16S rRNA amplification to be Pseudomonas aeruginosa PME1. Extracellular polymeric substances (EPS) of P. aeruginosa PME1 in the biofilm showed a reduction in total carbohydrate content (42%) with increased protein (9.0%), hexosamine (3.0%) and uronic acid (1.7%) content as compared to its planktonic form. Antimicrobial susceptibility testing revealed that P. aeruginosa PME1 biofilms were 17, 24, 27, 30, and 32 times more resistant to cefotaxime, imipenem, ceftazidime, tazobactam and piperacillin, respectively, than their free flowing counterparts.  相似文献