黄颡鱼（Pelteobagrus fulvidraco）鳃的超微结构研究     

罗芬  陈礼强  康斌 《海洋与湖沼》2011,42(4):488-494

应用光学和电子显微成像技术对黄颡鱼鳃的表面形态特征、鳃小片血管分布及其内部超微结构进行了观察。结果表明,黄颡鱼鳃耙呈长锥形,鳃弓表面具棘状小刺,鳃小片呈褶皱状,镶嵌排列在长条形鳃丝两侧。光镜可观察到鳃小片两侧红色的入鳃和出鳃动脉以及中部片状毛细血管网。鳃耙、鳃弓、鳃丝及鳃小片表面均被扁平上皮细胞覆盖,其表面微嵴形态结构...  相似文献   

长石莼（缘管浒苔）（Ulva linza）原生质体再生与分化发育初步研究          下载免费PDF全文

陈群芳  汤文仲  冯子慧  霍元子  何培民 《海洋与湖沼》2011,42(3):397-403

采用细胞酶解技术获得长石莼（缘管浒苔）原生质体,再通过细胞培养观察原生质体再生以及各种分化发育途径。结果表明,长石莼（缘管浒苔）主要存在3种分化发育途径：1）部分原生质体可直接分裂形成有假根或无假根得单细胞苗。2）部分原生质体分裂成规则细胞团或不规则细胞团,最后形成苗簇。3）部分原生质体可发育为孢子囊／配子囊,其中部分...  相似文献   

Establishment of a spleen cell line from large yellow croaker Pseudosciaena crocea and its primitive application in foreign gene transfection     

Ai Sun  Tingjun Fan  Xiuxia Yang  Bin Xu  Xiaohui Xu  Xueyang Guo 《中国海洋大学学报(英文版)》2011,10(4):397-401

A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco’s modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies.  相似文献   

湛江地区光裸星虫的生殖细胞发育和生殖周期   总被引：2，自引：0，他引：2  

王庆恒  杜晓东  黄洪艳  秦红贵 《广东海洋大学学报》2005,25(1):5-9

采用活体观察和常规石蜡切片技术对湛江地区光裸星虫生殖周期、生殖细胞的发育及形态结构进行研究。结果表明:星虫雌雄异体,无肉眼可见生殖腺结构,原生殖细胞在体腔液中发育;雌性生殖细胞根据其卵膜的形成和卵黄积累情况可以分为4个发育阶段,小生长期、大生长期、成熟前期和成熟期;雄性生殖细胞也分为增殖期、生长期、成熟期和变态期4个阶段;成熟的生殖细胞经肾管排出体外。生殖季节为5~9月,其中7~8月为高峰期。  相似文献   

虾池浮游微藻的种类组成、数量和多样性变动   总被引：6，自引：1，他引：5  

李卓佳  张汉华  郭志勋  贾晓平 《广东海洋大学学报》2005,25(3):29-34

报道了在集约化养虾中应用有益微生物制剂对虾池浮游微藻的影响,分析了整个养虾周期浮游微藻的种类组成、生物量、优势种及多样性指数的周期变化情况。结果表明,早期虾池浮游微藻细胞数量维持在1×103~3×103mL-1的水平,养殖中期开始呈指数增长状态,后期达到顶峰,并维持在70×103~160×103mL-1的水平,生物量比自然海区高。优势种类明显,前期为中肋骨条藻和伏氏海毛藻等硅藻类,后期则为绿球藻和栅列藻等绿藻类,水色及藻相呈良好状态,而不施加芽孢杆菌制剂的对照池则在后期出现较大比例的有害的蓝藻。虾池藻类多样性指数、均匀度指数和种类丰度均呈现前期高后期低的趋势,而优势度指数则相反,呈现前期低后期高的趋势。浮游植物的多样性指数为0.60~3.80,平均为1.88,均匀度指数为0.14~0.83,平均为0.45;种类丰度为0.80~2.46,平均为1.38;优势度指数在0.17~0.85,平均为0.41。多样性指数和均匀度均比粤东海域低,但优势度则甚高。  相似文献   

基于径向基函数神经网络的混合像元分解   总被引：10，自引：1，他引：10  

张彦  邵美珍 《遥感学报》2002,6(4):285-288

遥感图像中普通存在着混合像元。对这部分像元进行分类（即混合像元分解）是遥感图像处理中的难点。基于主分量分析的混合像元分解 法是一种较为成熟的算法，但它存在着计算量大，适应性差等缺点。在深入研究混合像元分解原理的基础上，提出了用径向基函数神经网络拟合分解结果超平面，以实现混合像元分解的算法，实验结果证明：该算法的结果与基于主分量分析的混合像元分解算法结果相近（相关系数达到0．00），而计算量大大减少，具有较强的适应性。  相似文献   

The Perspective of Deep-sea Science Research and the Establishment of Modern Hydrothermal Laboratories in China     

I-Ming CHOU 《地质学报》2013,87(Z1):3-4

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http://www.sciencedirect.com/science/article/pii/S1674987112001028     

Tatsuyuki Yamamoto  Hideo Akiyoshi  Keisuke Yoshikiyo  Tetsuya Takahashi  Yukiko Tanabe  Sakae Kudoh  Satoshi Imura  Naoyuki Yamamoto 《地学前缘(英文版)》2013,4(6):647-653

A study on the effect of the solar ultra-violet radiation on the human skin fibroblast cells revealed that the production of matrix metalloproteinase-2 was inhibited by the radiation.A CO2 incubator connected by optical fibers to a reflector telescope for collecting the solar light was built at Syowa station by the 49th Japanese Antarctica Research Expedition.The direction of the telescope was continuously controlled by a sun-tracker to follow the movement of the Sun automatically.The intensity of the collected light was monitored by a portable spectrophotometer housed inside.The human skin fibroblast cells were incubated in the CO2 chamber to investigate the effect of the solar radiation at Syowa station and were compared with those reference experiments at a laboratory in Japan.The results showed cell damage by strong UV radiation.The production of matrix metalloproteinase-2 was prompted by the moderate UV-B,but was inhibited by the strong UV-B radiation,as studied under laboratory conditions in Japan.The effect of strong solar radiation at Syowa station involving the radiation of UV-B region was estimated to be of the same extent of the radiation caused by an artificial UV-B light with the intensity more than 50 mJ/cm2.  相似文献   

“微观繁殖体”溯源     

沈颂东 《海洋与湖沼》2022,53(1):1-7

通过查阅"微观繁殖体"相关文献,对其概念进行溯源追踪并据以总结归纳。笔者认为"微观繁殖体"这个概念不应该作为放散的孢子、配子、合子、不同生长阶段的显微个体及有生长能力的大型海藻碎片的统称,而应只是泛指那些肉眼不可见的只进行营养繁殖的二倍体细胞团、脱落下来的组织块和"色素体"(有色藻段)等碎片,即是一个或者多个二倍体的藻类细胞。并以石莼属绿藻浒苔(Ulva prolifera)为例,简述浒苔微观繁殖体在其生活史中的主要作用,为浒苔"微观繁殖体"的理论研究和实际利用奠定基础。  相似文献   

Biosorption of Procion Red MX 5B by Bacillus subtilis and Its Extracellular Polysaccharide: Effect of Immobilization     

Arthur Raj Binupriya  Muthuswamy Sathishkumar  Chang Sub Ku  Sool Il Yun 《洁净——土壤、空气、水》2010,38(8):775-780

Bacillus subtilis and its extracellular polysaccharide (EPS) were used in free form as well as immobilized form as biosorbent for the removal of an anionic dye Procion Red MX 5B. Low pH was favourable for biosorption. Immobilization resulted in reduced biosorption of the dye. The presence of functional groups responsible for the high adsorption capacity in free cells (FC) and EPS was confirmed by FTIR analysis. High Q_max and b values were noted in the case of FC and free EPS in contrast to immobilized cells and EPS. The kinetics data showed that the adsorption system followed pseudo‐first‐order reaction at low dye concentration. Desorption of the dye was found to be 100% in 1 N NaOH. In the case of immobilized biomass and EPS the alginate was found to be unstable under high alkaline conditions of NaOH.  相似文献