The journal Communications in Numerical Methods in Engineering with Biomedical Applications becomes the International Journal for Numerical Methods in Biomedical Engineering (IJNMBE) from 1st January 2010     

Perumal Nithiarasu  Rainald Löhner 《国际地质力学数值与分析法杂志》2009,33(16):1769-1770

Communications in Numerical Methods in Engineering, founded by Roland W. Lewis in 1985, will change its title to the ‘International Journal for Numerical Methods in Biomedical Engineering’ and has a revised Aims and Scope. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE   总被引：1，自引：0，他引：1  

WEN Ruobing)  SUI Zhenghong)  *  ZHANG Xuecheng)  ZHANG Shuang)  and QIN Song) ) Laboratory of Marine Genetics  Breeding  College of Marine Life Sciences  Ocean University of China  Qingdao  P. R. China ) Institute of Oceanology  Chinese Academy of Sciences  Qingdao  P.R. China 《中国海洋大学学报(英文版)》2007,6(4):373-377

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.  相似文献   

Environmental effects on the reproduction and fecundity of the introduced calcareous sponge Paraleucilla magna in Rio de Janeiro,Brazil          下载免费PDF全文

Emilio Lanna  Rodolfo Paranhos  Paulo C. Paiva  Michelle Klautau 《Marine Ecology》2015,36(4):1075-1087

The calcareous sponge Paraleucilla magna (Porifera, Calcarea) has been the subject of several studies in the last decade. It was first described along the Brazilian coast, where it is considered cryptogenic, and was subsequently found in the Mediterranean, where it is considered invasive. The wide artificial distribution of this species allows us to compare different aspects of the biology of an introduced species in different locations. Here, we analysed the effects of selected environmental parameters on the reproductive dynamics of P. magna in Rio de Janeiro (Brazil) over 18 months and compared our results with those obtained for the same species in the Mediterranean Sea. Specimens were collected monthly and analysed through histological methods. The density of reproductive elements in each month was calculated, and the effects of environmental parameters (photoperiod, precipitation, temperature, phytoplankton and bacterioplankton) were analysed using a regression tree analysis. Paraleucilla magna was reproductive throughout the study period. The densities of the reproductive elements (oocytes, embryos and larvae) showed no seasonality, and this species presented one of the highest reproductive efforts documented to date in the phylum Porifera (99.0 oocytes · mm^?3; 89.0 embryos · mm^?3; 319.0 larvae · mm^?3). The main environmental parameters related to the reproduction of P. magna were temperature, photoperiod and bacterioplankton. Temperature was the main driver associated with the densities of oocytes and embryos, while bacterioplankton was the main driver of larvae (positive relationships). In Rio de Janeiro, larvae were present and continuously released. This strategy is different from that observed in the Mediterranean, where a larger larval output was observed but only during the summer months. Our results show that P. magna is a species with a strong invasive potential, considering its high and continuous reproductive effort. This high fecundity stimulated by high temperatures may be a key factor contributing to the growth of P. magna populations and its invasion of new areas.  相似文献   

南海深海鱼类一新记录种——鞭尾鱼          下载免费PDF全文

江艳娥  张俊  陈作志  蔡研聪  孔啸兰  杨玉滔  黄梓荣 《应用海洋学学报》2022,41(4):565-567

2016年11月至2020年7月在南海海域采集管眼鱼6尾,经形态特征鉴定为月鱼目、鞭尾鱼科、鞭尾鱼属(Stylephorus)、鞭尾鱼(Stylephorus chordates)。鞭尾鱼主要鉴别特征为：体长,侧扁。眼大,呈望远镜状,朝前或朝上。吻小,管状,口有特别大的伸缩能力。背鳍从颈背部延伸至尾鳍基部。尾鳍分明显的上叶和下叶,下叶2鳍条极度延长。鞭尾鱼在南海海域的发现是该科、该属、该种鱼类在中国海域的首次纪录。  相似文献   

基于线粒体控制区的中国东南沿海黄鳍棘鲷群体遗传多样性和遗传分化          下载免费PDF全文

叶幼玲  黄张帆  徐安乐  汪睛  黎中宝 《应用海洋学学报》2022,41(4):583-590

本研究通过对线粒体控制区序列进行分析的技术手段,对中国宁德（ND）、厦门（XM）、漳浦（ZP）、南澳岛（NAD）、湛江（ZJ）、海口（HK）与北海（BH）的7个野生黄鳍棘鲷（Acanthopagrus latus）群体的遗传多样性和遗传分化进行比较分析。结果表明：中国东南沿海的野生黄鳍棘鲷群体呈现出中等以上的遗传多样性特征,48个黄鳍棘鲷样品的控制区序列长度为580 bp,单倍型数目为44个,总体的单倍型多样性指数（Hd）和核苷酸多样性指数（Pi）分别为0.996和0.017 30。7个野生黄鳍棘鲷群体间的遗传分化程度较低,各群体间的遗传距离为0.010 28~0.029 87。群体间的遗传分化系数（FST）为-0.068 41~0.545 86,北海群体和其他群体间存在一定程度的遗传分化。AMOVA分析显示群体内的遗传变异占比为83.34%,大部分遗传变异发生在组群内;但北海群体与其他6个群体分为两个组群后,组群间的遗传变异占比为42.66%,两个组群间存在较大程度的遗传变异。基于Kimrua距离法构建的系统进化树中显示北海群体聚为一个分支,另外6个群体聚为一支。基于TCS network构建的单倍型网络图中44个单倍型混杂在一起,未按照水系格局和地理距离进行分布,表明黄鳍棘鲷群体间未形成多个单系群。本研究可为中国东南沿海野生黄鳍棘鲷群体的种质资源利用和保护提供科学依据。  相似文献   

铁还原过程对栖热腔菌Thermosipho ferrireducens JL129W03^T的厌氧发酵代谢影响          下载免费PDF全文

陈瑶  邵宗泽  邱冬华  杨渐  曾湘 《应用海洋学学报》2022,41(4):591-598

本研究以分离自西北印度洋深海热液硫化物的铁还原栖热腔菌(Thermosipho ferrireducens) JL129W03T为研究对象,在以四方纤铁矿(β FeOOH)为电子受体条件下,测定了不同培养时间的细胞生长量和主要的代谢产物,并结合其基因组信息预测了主要代谢途径。结果表明,厌氧糖酵解是该菌的主要代谢途径,发酵代谢产物包括乙醇、乙酸、乳酸、丁酸和CO2。同时,该菌含有\[Fe Fe\]型氢酶,可以利用厌氧发酵过程产生的还原态铁氧还蛋白获得电子产生H2。在培养基中添加四方纤铁矿后,促进了菌株的最大细胞生长量(3.17×107 cells/mL至2.19×108 cells/mL),也促进了菌株对淀粉和纤维素的利用率(分别提高了100%和34%)。同时,培养基中添加四方纤铁矿后,菌株JL129W03的乙醇产量增加了76%,而丁酸的产量却降低了73%,对H2、乙酸、乳酸的产生影响较小。本研究对了解Thermosipho ferrireducens JL129W03T的生理代谢特点和进一步应用开发清洁能源(H2、乙醇等)奠定了基础。  相似文献   

乳酸乳球菌XP-15对仿刺参生长、免疫及肠道菌群结构的影响          下载免费PDF全文

杨求华 《应用海洋学学报》2022,41(3):407-413

乳酸乳球菌（Lactococcus lactis）是一种具有促进宿主生长、增强机体免疫力的益生菌。本研究以仿刺参（Apostichopus japonicus）为研究对象,以仿刺参源肠道分离菌株乳酸乳球菌XP 15为候选益生菌,开展菌株XP 15对仿刺参生长、免疫及肠道菌群结构的影响研究。以乳酸乳球菌XP 15投喂组为实验组（XP 15组）,基础饲料投喂组为对照组（CK组）,进行为期8周的养殖实验。结果显示,第1—8周,XP 15组的仿刺参肠道内的乳球菌属（Lactococcus spp.）呈现逐渐增加趋势,至第8周时占比为54.00%,明显高于CK组的7.33%,表明菌株XP 15可较好地在仿刺参肠道内定殖;于第6周取肠道进行免疫酶活力测定,结果显示,XP 15组仿刺参肠道一氧化氮合成酶（nitric oxide synthase, NOS）、酸性磷酸酶（acid phosphatase, ACP）和超氧化物歧化酶（superoxide dismutase, SOD）等免疫指标显著高于CK组。于第6周采用体腔注射法进行相对免疫保护率实验,计算结果表明,投喂菌株XP 15后仿刺参在塔式弧菌（Vibrio tubiashii）感染中的成活率为73.33%,高于对照组的33.33%。于第8周测定各组仿刺参体重计算增重率,结果显示,初始体重为(43.63±2.15) g的XP 15组仿刺参的增重率为122.19%,显著高于初始体重为(42.10±1.93) g的CK组(增重率为109.46%）,表明菌株XP 15对仿刺参的生长有一定的促进作用。本研究可为乳酸乳球菌作为候选益生菌种在水产养殖上的应用提供参考依据。  相似文献   

海洋来源羽毛降解菌株的筛选、鉴定及酶学性质研究          下载免费PDF全文

张红岩  何瑾  杨梦莹  张妮  王芳  申乃坤 《应用海洋学学报》2022,41(2):338-346

微生物降解羽毛既可保护环境又能实现废物的资源化利用,具有显著的经济效益和社会效益。本研究从广西钦州港海鸭养殖淤泥筛选获得一株可高效降解羽毛的菌株Gxun-20,通过对该菌株形态特征、生理生化特性和16S rRNA基因序列进行分析,以确定菌株的分类,并对菌株产角蛋白酶的条件及酶学性质进行研究。结果表明：该菌为副短短芽孢杆菌(Brevibacillus parabrevis);菌株最适产酶条件为：温度34 ℃,初始pH 6.5,羽毛含量1.5%(质量分数),发酵48 h,角蛋白酶活性可达277.45 U/mL。菌株Gxun-20所分泌的角蛋白酶最适温度为50 ℃,且热稳定性较好,最适pH为7.5;金属离子Fe³⁺、Cu²⁺、Co²⁺、Fe²⁺和Mn²⁺对酶活性有显著抑制作用,而Ca²⁺、Na⁺对酶活性有明显促进作用;金属螯合剂乙二胺四乙酸(ethylene diamine tetraacetic acid, EDTA)可显著抑制酶的活性,蛋白酶抑制剂苯甲基磺酰氟(pheny methyl sulfonyl fluoride, PMSF)可完全抑制酶活性,表明该蛋白酶的活性中心含有金属离子和丝氨酸;异丙醇可使酶活性提高至129.34%,酶活性在十二烷基硫酸钠(sodium dodecyl sulfate, SDS)、吐温40溶液中保持稳定;酶的底物特异性结果表明,该酶对羽毛类β-角蛋白及酪蛋白降解能力较好,而对牛血清蛋白、I型胶原及人发类α 角蛋白降解能力较弱。海洋来源的副短短芽孢杆菌Gxun-20及其所产角蛋白酶在废弃羽毛降解方面具有良好的应用价值。  相似文献   

深海来源芳烃类降解菌筛选与盐单胞菌芘降解能力测定          下载免费PDF全文

李振宇  尹华群  邵宗泽  王万鹏 《应用海洋学学报》2022,41(2):328-337

海洋来源的菌株在高盐污水类修复处理中有重要的应用前景,而烃类物质是污水中的重要组成部分,其中含有多个苯环的芳香烃族化合物,对人类和环境具有较大的毒性,而目前关于多环芳烃(PAHs)厌氧代谢的相关报道相对较少。本研究以PAHs(萘、菲和芘等)为唯一碳源和能源,通过厌氧富集对太平洋深海沉积物中的PAHs降解菌展开研究。富集物细菌菌群分析结果表明,盐单胞菌属(Halomonas)、海旋菌属(Thalassospira)、海杆菌属(Marinobacter)、海洋杆菌属(Oceanobacter)和食烷菌属(Alcanivorax)等是主要的功能类群;其中盐单胞菌属是最主要的功能类群。通过筛选分离获得一高效烃降解盐单胞菌株,鉴定并命名为泰坦尼克盐单胞菌(Halomonas titanicae) PA16-9,该菌与模式菌株Halomonas titanicae BH1^T的16S rRNA相似性为99.52%。基于16S rRNA基因序列比对搜寻NCBI数据库,发现Halomonas titanicae广泛存在于废水、活性污泥、油田、湿地等有机质复杂区域,生态位分布极广,可利用丰富多样的底物。通过生长实验表明,菌株PA16-9能在厌氧条件下以硝酸盐为主要电子受体,利用芘、苯并芘和十六烷等烃类物质为唯一碳源进行生长,在培养45 d后芘的降解率达到61.9%,期间亚硝酸盐逐渐积累,浓度达到约0.24 mmol/L。盐单胞菌广泛存在于厌氧烃降解富集菌群中,暗示了盐单胞菌属在厌氧烃代谢中可能发挥了重要作用,在实际应用上具有很大潜力。  相似文献   

基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析          下载免费PDF全文

王方晗  王雷  孙婷婷  董志军 《应用海洋学学报》2022,41(2):317-327

西沙群岛是我国南海陆地面积最大的群岛,海域广阔,拥有重要的战略地位与丰富的海洋资源。为了解该海域浮游动物组成与分布特征,本研究于2019年5月在西沙群岛14个岛礁站位开展多学科综合调查,并采用形态学方法和基于18S V9测序的宏条形码技术对浮游动物样本组成进行鉴定。结果显示,此次西沙调查站位浮游动物样本的主要种类包括桡足类、软甲纲和箭虫纲,这3个类别的物种在两种鉴定方法中均具有较高的相对丰度。14个站位浮游动物平均密度为707.53±378.34 ind/m³,各站位浮游动物丰度、物种组成及优势种存在差异。形态学方法共鉴定出11门17纲18目共86个物种,18S V9分子方法鉴定出22门46纲85目共233个操作分类单元(operational taxonomic units, OTUs),分子鉴定的物种覆盖度更高,且代表性类群相对丰度和多样性指数在大部分站位与形态学鉴定结果呈现出显著相关性,表明宏条形码技术鉴定方法与形态学鉴定方法在评价海洋浮游动物多样性方面具有较好的可比性,在我国海洋浮游动物群落监测中具有较高的应用潜力。但由于目前浮游动物的分子鉴定方法仍处于初步发展阶段,相关技术手段仍不完善,仍需多种鉴定方法结合使用,以保证浮游动物多样性鉴定的准确性。  相似文献