Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Body weight, sexual maturity, and spinal deformity in strains and families of Atlantic cod (Gadus morhua) at two years of age at different locations along the Norwegian coast     

Kolstad  K.; Thorland  I.; Refstie  T.; Gjerde  B. 《ICES Journal of Marine Science》2006,63(2):246-252

  相似文献   

波群作用于垂直桩柱的峰力统计性质     

徐立论  陈志军  焦海英 《中国海洋大学学报(自然科学版)》1994,(1)

研究具有窄谱和Ｗｅｉｂｕｌｌ波高分布的波群对非线性桩柱系统作用力的统计性质。求得了桩桩的波浪峰力的各种特征值及其比值。指出这些数值不仅是阻力一惯性力参数ｂＨ的函数，也随着波群因子而变化。本文模式更具广泛性。文中给出了一系列计算图表，可从理论计算波群作用于桩柱的波浪峰力。  相似文献   

A study of Coriolis effects on long waves propagating in an unbounded ocean, channel and basin     

Ashabul Hoque  Azizur Rahman 《Ocean Engineering》2007,34(11-12):1701-1705

The effects of Coriolis force on long waves have been discussed based on gravity waves propagating in an unbounded ocean, channel and basin. In case of ocean, results show that the Coriolis effect will be significant and negligible, when the wave period is comparable to 2π/f and much shorter, respectively. Results also show in a channel, the wave amplitude and water particle velocity decrease exponentially in the positive y direction in the northern hemisphere (where f is positive). Moreover, in a basin, the Cotidal lines have been found as curves and rotate counterclockwise around the origin.  相似文献   

An experimental method for determining the frequency-dependent added mass and added mass moment of inertia for a floating body in heave and pitch motions     

Jong-Shyong Wu  Mang Hsieh   《Ocean Engineering》2001,28(4):528

Most of the existing relevant materials have been obtained from experiments, in which evaluating the added mass at the resonant frequency corresponding to the peak of a frequency-response curve obtained from the “forced” vibration analysis is the most popular technique. In this paper, a simple experimental method was presented where the “free” vibration responses instead of the “forced” ones were used to determine the values of m_ah and I_ap. The main part of the experimental system is composed of a floating body (model) and a spring–shaft shaker. The “free” vibration of this main part was induced by imposing on it an initial displacement (and/or an initial velocity), and from the time histories of displacements information such as the “damped” natural frequencies, damping ratios, sectional added mass coefficients (C_V and C_P) were obtained. Since the displacements of the spring–shaft shaker are “translational” and those of the floating body due to pitch motions are “angular”, a technique for the transformation between the associated parameters of the two components of the main part was presented.  相似文献   

波浪底摩擦力对重砂富集的影响——以鲁南近岸区为例     

金秉福 《海洋科学》1997,21(5):49-52

根据浅水波浪的底摩擦效应，对鲁南灵山湾和臼－岚山镇近岸区进行了波浪的海底摩擦力计算。波浪的海底摩擦力等值图与已知滨海锆石砂矿资料对比表明，底摩擦力大于０．１８Ｎ／ｍ＾２的动力分选区有利于重矿物砂富集。据此，进一步预测了鲁南近岸带的砂矿富集区。  相似文献   

McCreary和Fickian湍扩散形式差异的分析     

吴德星  李国璋  李宝辉  陈峰 《中国海洋大学学报(自然科学版)》1993,(Z2)

用矩阵方法,求解McCreary和Fickian铅垂向湍扩散形式下,无界海洋条件时在风应力和深层浮力作用下的线性解。得出不同的湍扩散系数A情况下McCreary与Fickian湍扩散形式的流场结构。指出随湍扩散系数的增加,McCreary和Fickian湍扩散形式下u分量结构及其量值差异增大。在Fickian湍扩散形式下,能量穿越密度跃层的传递能力较强。当A=0.1cm~2s~(-3)时,在风应力和深层浮力作用下两种湍扩散形式造成流场的差异很小。提出在小湍扩散系数下,用McCreary湍扩散形式研究热带海洋环流,其结果和Fickian形式应相当一致。  相似文献   

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures   总被引：4，自引：1，他引：4  

SHI  Qingzeng 《中国海洋工程》2002,16(2):183-192

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.  相似文献   

抑制栉孔扇贝第一极体对受精卵染色体行为及胚胎倍性组成的影响   总被引：4，自引：0，他引：4  

李永仁  阙华勇  张国范 《海洋科学》2005,29(8):66-70

采用细胞松弛素B（CB）处理，栉孔扇贝（Chlamys ferreri）抑制其受精卵的第一极体（PB1），研究抑制PB1对受精卵减数分裂过程及胚胎倍性组成的影响。结果发现，抑制第一极体显著改变了受精卵的染色体行为，在第二次减数分裂过程中共发现4种典型染色体分离类型，分别是三极分离（41．7％）、二极分离（11．7％）、双二极分离（24．9％）和非同步分离（2．8％），其余的受精卵（19．0％）染色体分离行为紊乱。对4-8细胞期胚胎的倍性组成进行分析，发现处理组中含有二倍体（10．9％）、三倍体（12．5％）、四倍体（19.5％）、五倍体（12．6％）以及非整倍体（46．6％）胚胎。研究结果表明，二极分离和双二极分离分别是形成三倍体和四倍体的主要机制，而其他的染色体分离行为将主要形成非整倍体。  相似文献   

台湾东北海域冷水块的特征     

孙湘平  修树孟 《海洋科学进展》2002,20(1):1-10

根据海洋调查历史资料 ,对台湾东北海域冷水块的时空分布、结构、特点进行分析 ,主要结果如下 :(1 )首次利用卫星跟踪漂流浮标的轨迹 ,佐证冬季该冷水块的存在。 (2 )鉴于该冷水的重要性 ,建议对它给予称谓 ,暂定名为“彭花棉”冷水块。 (3 )该冷水块是黑潮次表层水涌升的结果 ,是黑潮对我国近海海洋环境影响的典型实例之一。 (4)该冷水块具有低温、高盐、高密、低氧、高磷、高硅和高氮等特性  相似文献