藻类基因启动子结构与功能研究进展     

张学成  郭楠  宋晓金 《中国海洋大学学报(自然科学版)》2008,38(3):404-412

启动子是基因表达调控重要的顺式元件,其结构与功能的研究成为分子生物学的研究热点之一.本文介绍了植物基因启动子的研究方法与概况,重点综述了藻类基因启动子的研究现状,以及本实验室对节旋藻藻蓝蛋白基因启动子的研究情况,并就启动子研究在基因工程中的应用价值和存在问题进行了讨论.  相似文献   

高通量测序分析北仑河口红树林潮间带底栖真核生物分布          下载免费PDF全文

吴鹏  时小军  吕向立  高阳  李海涛  张敬怀 《应用海洋学学报》2019,38(2):232-238

为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。  相似文献   

海洋溢油污染对生物群落和种群的影响及生态系统的恢复   总被引：1，自引：0，他引：1  

景伟文  杨桂朋  康志强 《海洋湖沼通报》2008,(1):80-89

海洋溢油污染带来的最严重的威胁在于它能够改变或破坏海洋环境中正常存在的生态系统。溢油污染对生态系统的初步影响是造成生物物种多样性、丰度、均匀度下降,进一步则是敏感物种消退,另一些机会物种大量繁殖,群落结构受到扰动。受到溢油污染后的生物群落的变化和恢复过程通常呈现的是多种因素共同作用的结果。溢油作为一种外来的扰动因素,对生态系统的发展强行加注了一种相对统一的发展模式,生态系统会经历一些优势种之间强烈的相互作用,种群数量出现大幅度的波动,系统变得敏感脆弱,生态恢复需要一定的时间。本文对国内外几十年来的研究成果进行综述,总结今后应大力开展海洋石油污染调查研究工作的各个方面。  相似文献   

诏安湾及毗邻海域浮游动物的群落结构          下载免费PDF全文

戴红  董炜峰  陈瑶  陈章飞  戴桂香  颜露露 《应用海洋学学报》2018,37(1):135-140

根据2015年夏(8月)、秋(10月)2季在诏安湾及毗邻海域开展的30个站浮游动物监测资料,研究了该海域浮游动物的种类组成、数量分布和多样性.结果显示:共鉴定浮游动物103种和浮游幼体24类,桡足类为绝对优势的类群.优势种有14种,锥形宽水蚤(Temora turbinata)、异体住囊虫(Oikopleura dioica)和强额孔雀水蚤(Parvocalanus crassirostris)是该海域重要的优势种群.生态类群以近岸暖水类群和外海广布类群为主.夏季浮游动物丰度平均为761个/m3,秋季为81个/m3;夏季浮游动物生物量平均为237mg/m3,秋季为52 mg/m3.夏季浮游动物种类数、丰度、生物量及变化幅度均明显高于秋季,夏季湾外浮游动物种类数、丰度、生物量及变化幅度均明显大于湾内,秋季湾外浮游动物种类数、丰度及变化幅度均明显大于湾内,夏、秋季浮游动物种类数和丰度总体分布均呈现由湾内往湾外逐渐增加趋势.夏季浮游动物多样性指数平均为2.46,秋季为3.14.与历史资料比较:本次调查海域浮游动物种类数和平均丰度均有较大幅度升高,但平均生物量有较大幅度下降.  相似文献   

杭州湾上海石化沿岸潮间带生态环境分析   总被引：1，自引：0，他引：1  

张进龙 《海洋湖沼通报》2008,(1):74-79

通过对1993～2002年杭州湾上海石化沿岸潮间带底栖动物群落结构的分析,得出上海石化处理达标后排放的工业废水对此段潮间带生态环境影响的范围和程度。自2002年到现在为止,本潮间带的底栖动物群落结构呈现一种适应性广、耐污染的结构组成.其生物多样性指数值在2.00～3.50之间。  相似文献   

A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China     

YANG Bo  OUYANG Jianping  AO Jingqun  CHEN Xinhua 《海洋学报(英文版)》2009,28(1):62-71

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.  相似文献   

Isolation and identification of bacteria associated with the surfaces of several algal species     

王子峰  肖天  逄少军  刘敏  岳海东 《中国海洋湖沼学报》2009,27(3):487-492

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on ...  相似文献   

Synthesis,algal inhibition activities and QSAR studies of novel gramine compounds containing ester functional groups     

李霞  于良民  姜晓辉  夏树伟  赵海洲 《中国海洋湖沼学报》2009,27(2):309-316

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Mapping the farming-pastoral ecotones in China     

Jian Zhang  Jie Wei  Quangong Chen 《山地科学学报》2009,6(1):78-87

Conventional farming-pastoral ecotones methods of delineating were not quantitative and could not fully show their spatial distribution. The present paper attempts to develop quantitative methods for mapping farming-pastoral ecotones in China. Nine indicators, related to temperature, precipitation and altitude aspects, were selected to quantify ecological susceptibility of vegetation （crops and forage）. Methods of analytic hierarchy process （AHP） and expert score ranking combined with fuzzy set theory were applied to assign the weight for each indicator and to define the membership functions. The geographic information system （GIS） was used to manage the spatial database and conduct the spatial analysis. According to the spatial calculation of evaluation model integrated with GIS, the ecological susceptibility of vegetation （crops and forage） was mapped. Three different zones, pastoral area, farming-pastoral ecotones and farming area, were classified by spatial cluster analysis and the maximum likelihood classification for the numeric map of vegetation ecological susceptibility by GIS. This map was validated by the economic statistical result based on the ratio of the output value from animal husbandry in total output value of agriculture by the National Bureau of Statistics in China, indicating that the mapping of the farming-pastoral ecotones may be accepted.  相似文献   

福建沙埕港浮游动物群落特征及影响因子   总被引：1，自引：0，他引：1  

朱延忠  陈洪举  刘光兴 《中国海洋大学学报(自然科学版)》2008,38(6)

根据沙埕港2006年1月和4月冬、春季2个航次的调查资料,分析了浮游动物种类组成、优势种、丰度和生物多样性的季节变化,并利用PRIMER统计软件中的PCA、BIOENV和RELATE程序探讨了浮游动物与环境因子的关系.经鉴定共有浮游动物77个种类(含浮游幼虫15类 ).中华哲水蚤(Calanus sinicus)和真刺唇角水蚤(Labidocera euchaeta)是2个航次共有的优势种,桡足幼体在2个航次中也都占优势.与1990年调查相比,浮游动物丰度大幅下降,浮游动物种类组成已发生了较大改变.沙埕港的浮游动物大致可分为河口型类群、沿岸型类群和近海型类群.冬季航次浮游动物的平均总丰度为20.6 ind/m3,春季航次为92. 2 ind/m3,低于福清湾浮游动物同期调查结果.浮游动物总丰度分布均为由湾口向湾顶递减的趋势.春季的浮游动物总丰度和香农-威纳多样性指数均高于冬季,冬季浮游动物生物多样性呈现湾中、西部高,湾口、湾顶低的趋势,而春季浮游动物多样性分布比较均匀.经统计分析,影响冬季和春季浮游动物分布的主要环境因子为盐度、温度.  相似文献