Application of quantitative PCR method in detection of Lymphocystis disease virus China （LCDV-cn） in Japanese flounder （Paralichthys olivaceus）     

昝金东  孙修勤  张之文  曲凌云  张进兴 《中国海洋湖沼学报》2007,25(4):418-422

Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder.  相似文献   

基于Kongsberg EM多波束的Snippet数据处理方法     

胡俊  吴永亭  豆虎林  李治远 《海洋科学进展》2020,38(4):717-724

多波束声呐记录的海底后向散射片段(Snippet)数据处理成角度响应曲线和地理编码(Mosaic)图像可以 帮助识别海底底质类型和反映地貌形态,这一过程包括辐射校正、角度响应改正(AVG)和几何地理编码,但不同的多波束系统硬件在辐射校正和角度响应改正方法上存在差异且传统处理方法忽略了声呐系统本身的指向性模型随时间变化的事实。以声呐方程为基础,针对Kongsberg EM 多波束系统提出了一套完整的Snippet数据处理流程,并分析了各步骤中存在的可变性,给出了每一步的处理建议,最后将此方法应用于EM2040浅水多波束实测数据,并验证了该方法的有效性和实用性。  相似文献   

鄂霍次克海南部晚第四纪的古海洋学记录   总被引：1，自引：0，他引：1  

孙烨忱  王汝建  陈建芳  高爱国  李秀珠  韩贻兵 《海洋地质与第四纪地质》2009,(2)

鄂霍次克海是太平洋第二大边缘海,在西北太平洋水文环境中扮演重要角色。综合分析了鄂霍次克海南部T00孔沉积物的多种替代性指标,揭示了鄂霍次克海晚第四纪以来的环境变化受季节性海冰变化、大气循环模式、陆源物质通量和表层生产力的共同影响。对比放射虫Cycladophora davisiana的含量曲线与LR04氧同位素记录,该孔沉积物可划分为氧同位素1-7期,底部年龄约为250ka。C.davisiana在间冰期的高含量表明鄂霍次克海中层水是北太平洋中层水的主要源区。蛋白石和有机碳的分析显示鄂霍次克海表层生产力在冰消期突然增大,随后在间冰期逐渐下降,冰期普遍较低。C/N比值曲线的分析说明鄂霍次克海的有机质沉积物主要来源于海洋。沉积物粒度的分析揭示鄂霍次克海冰期时陆源粗颗粒含量较低,至冰消期粗颗粒含量突然增加,而在间冰期陆源粗颗粒含量较高。  相似文献   

东海内陆架表层沉积物粒度及其净输运模式   总被引：2，自引：0，他引：2  

刘升发  刘焱光  朱爱美  李朝新  石学法 《海洋地质与第四纪地质》2009,(1)

根据对东海内陆架海底沉积物的粒度测试,运用Gao-Collins"粒度趋势分析"方法,探讨了东海内陆架海底表层沉积物的粒度分布特征和净输运趋势。结果表明,研究区表层沉积物可以分为两类:细颗粒(>5Φ)和粗颗粒(<5Φ);细颗粒分布于研究区的大部分区域,而粗颗粒主要分布在闽江、瓯江等入海河流口门以及马祖等海岛附近;表层沉积物的平均粒径、分选系数、偏态等粒度参数对应性较好,总体特征为粒径越粗,分选越差,偏态更正偏,而粒径越细,分选越好,偏态也较低;粒径趋势分析显示东海陆架表层沉积物的运移模式总体为NE—WS方向运移,而研究区边缘在运移过程中发生向左右两侧的偏移,南部闽江河口区沉积物由近岸向海方向辐射运移,研究区表层沉积物的净输运模式主要受控于流系和地形等多种因素。  相似文献   

Evidence for the 8,200 a <Emphasis Type="SmallCaps">b</Emphasis>.<Emphasis Type="SmallCaps">p</Emphasis>. cooling event in the middle Okinawa Trough     

Hua?YuEmail author  Yingqian?Xiong  Zhenxia?Liu  Serge?Berné  Chi-Yue?Huang  Guodong?Jia 《Geo-Marine Letters》2008,28(3):131-136

Based on new and existing data on oxygen isotopes, alkenone-surface seawater temperature trends, planktonic foraminifers, lithology, and clay mineral composition of piston cores, a distinct cooling event has been identified around 8,200 cal a b.p. in the middle Okinawa Trough, northwest Pacific. This corresponds to the 8,200 a b.p. cooling event recorded in many places of the Northern Hemisphere. During this event, the local temperature decreased by 1°C, and the δ¹⁸O value increased by 0.6‰. A strengthened Asian winter monsoon is the most probable cause for this event, which thus adds further credibility to the contention that we are dealing here with a global phenomenon.  相似文献   

The effect of the wave-induced mixing on the upper ocean temperature in a climate model   总被引：3，自引：1，他引：2  

HUANG Chuanjiang  QIAO Fangli  SONG Zhenya 《海洋学报(英文版)》2008,27(3):104-111

The significant underestimation of sea surface temperature （SST） and the temperature in the upper ocean is one of common problems in present climate models. The influence of the wave-induced mixing on SST and the temperature in the upper ocean was examined based on a global climate model. The results from the model coupled with wave-induced mixing showed a significant improvement in the simulation of SST and the temperature in the upper ocean compared with those of the original model without wave effects. Although there has still a cold bias, the new simulation is much closer to the climatology, especially in the northern ocean and tropical ocean. This study indicates that some important physical processes in the accurate simulation of the ocean may be ignored in present climate models, and the wave-induced mixing is one of those factors. Thus, the wave-induced mixing （ or the effect of surface waves） should be incorporated properly into climate models in order to simulate or forecast the ocean, then climate system, more accurately.  相似文献   

西南印度洋中脊表层沉积物非碳酸盐组分的粒度特征及其地质意义   总被引：1，自引：0，他引：1  

贾琦  范德江  孙晓霞  刘明  张文强  杨作升 《海洋学报(英文版)》2019,38(2):34-43

The carbonate-free fraction of 20 surface sediments collected from the ultraslow-spreading Southwest Indian Ridge(SWIR) was studied by grain size analysis and mineralogical analysis with X-ray powder diffraction(XRD),stereo microscopy and scanning electron microscopy(SEM). The characteristics of the carbonate-free fraction of the sediments were obtained, and related influential factors were discussed. The results show that the mean grain size of this fraction is in 1.96Φ–8.19Φ, with poorly sorting and unimodal, bimodal or irregular bimodal distribution patterns. Four grain size end members of the fraction are derived with the End Member Model method. The finest end member EM1 shows a significant contribution of terrigenous materials of the aeolian input and sediment carried by the bottom current. End member EM2 with medium size mainly reflects sediment of a siliceous bioclast origin. EM3 and EM4 are interpreted as representing the coarser volcanic materials related to bedrock weathering or volcanic activities. Multi-provenance is the dominant factor controlling the grain size pattern of the carbonate-free fraction of the sediments in that area. In addition, sediment transport processes such as the bottom current and wind are the minor factors that influence the grain size distribution of the carbonate-free fraction sediments.  相似文献   

东印度洋海域最优深度基准面模型构建     

姜传苓  付延光  周兴华  陈义兰  王永康 《海洋科学进展》2020,38(2):326-335

利用东印度洋海域周边长期验潮站实测数据、TOPEX/Poseidon等系列卫星测高反演结果,评估了DTU10,EOT11a,FES2014,GOT4.8,OSU12和TPXO8六种全球潮汐模型精度,根据卫星测高结果给出了浅水分潮改正量和长周期分潮改正量的经验模型,又在此基础上分析并构建了研究区域精度最优的深度基准面模型。考虑到全球潮汐模型在近岸的影响因素及验潮站位置,将13个验潮站分成开阔海域与近海海域两类,与潮汐模型的对比,结果表明,DTU10和FES2014模型分别在开阔海域和近海海域精度最优。根据潮汐模型在不同分潮处的精度,如EOT11a模型在O1和K1分潮处精度较高,DTU10在N2,M2,S2和K2分潮处精度较高等,分别构建了开阔海域与近海海域的组合深度基准面模型,计算得知误差分别为11.33和20.95 cm,其精度显著提高。  相似文献   

Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil     

蒋凤华  张丽  杨佰娟  郑立  孙承君 《中国海洋湖沼学报》2015,33(4):853-861

To investigate the effect of the water soluble fraction of crude oil(WSF) on marine bivalves, the scallop C hlamys farreri was exposed to three WSF concentrations(0.18 mg/L, 0.32 mg/L, and 0.51 mg/L, respectively) in seawater. Petroleum hydrocarbon contents in scallops and a suite of enzymes [7-Ethoxyresorufin-O-deethylase(EROD), aryl hydrocarbon hydroxylase(AHH), glutathione S-transferase(GST), and glutathione peroxidase(GPx)] in gills and digestive glands were monitored over 10 days. The results revealed that WSF affected the activity of the four enzymes in the gills and digestive glands. EROD activity in the gills was significantly induced in most individuals of the three test groups, while in the digestive gland it was significantly induced in the low-concentration group within 4 days but was inhibited in the middle- and high-concentration groups on days 1, 4, and 10. AHH activity in the gills of all treatment groups was significantly induced on day 1. In the digestive gland, AHH activity was induced in most individuals from the treatment groups. In all treatment groups, GST activity was significantly inhibited from days 2 to 10 in the gills and was induced after day 4 in the digestive gland. GPx activity in the gills was significantly inhibited throughout the exposure period in all treatment groups. There was no overall significant difference in GPx activity in the digestive gland between the control and treatment groups. Our results also revealed that petroleum hydrocarbon concentrations in the tissues increased linearly with exposure time. EROD activity in the digestive gland and GST and GPx activity in the gill tissue were negatively correlated with petroleum hydrocarbon body burden. These enzymes play important roles in detoxification and can act as potential biomarkers for monitoring petroleum hydrocarbon contaminants in the marine environment.  相似文献   

近十年来渤海湾围填海工程对渤海湾水交换的影响   总被引：2，自引：0，他引：2  

王勇智  吴頔  石洪华  赵晓龙  闫文文 《海洋与湖沼》2015,46(3):471-480

近十年来我国海岸带地区快速城市化,对土地的需求持续高涨,导致沿海地区围海造地现象愈演愈烈,海湾纳潮海域面积持续萎缩,对海湾的水交换带来了较大压力。为此,选取我国海岸带快速城市化的典型区域——渤海湾为例,研究近十年来渤海湾海岸带城市化对渤海湾水交换的影响。基于国际流行的MIKE3水动力模型耦合粒子追踪模块,应用2000年和2010年两个典型年份的渤海湾岸线资料,分别计算了2000年和2010年渤海湾环流系统及其驱动下的水质点运移规律和变化特征。研究将渤海湾划分为8个区块,定量化研究和对比了近十年来由于渤海湾持续围填海导致的海湾水存留时间和不同区块水交换能力的变化。结果表明,随着近十年来渤海湾沿岸快速城市化产生的岸线变化,导致湾内环流涡旋增多,决定湾内水交换的流场结构发生了显著变化,导致各区块的水交换能力和平均水存留时间不仅存在较大差异,而且发生了显著的变化。与2000年相比,2010年天津港、黄骅港和曹妃甸港区海域的平均水存留时间明显增加,水交换率下降,对区域水质的改善十分不利。  相似文献