瓦氏黄颡鱼(Pelteobagrus vachellii)Toll样受体2(TLR2)基因克隆及免疫功能     

覃川杰  龚全  文正勇  袁登越  王均  贺扬  邵婷 《海洋与湖沼》2018,49(1):198-206

为探究Toll样受体2(Toll-like receptor 2,TLR2)及下游免疫分子对瓦氏黄颡鱼(Pelteobagrus vachellii)机体的保护作用,本研究采用RT-PCR及RACE法获得瓦氏黄颡鱼TLR2全长c DNA(2611bp),编码789个氨基酸残基,含有10个富含亮氨酸的重复序列(Leucine Rich Repeat,LRR)和Toll/IL-1R(Toll/IL-1 Receptor Domain,TIR)同源区结构域,属I型跨膜受体。序列同源性比对发现,瓦氏黄颡鱼TLR2 c DNA与斑点叉尾、鲤及虹鳟的同源性分别为78%、62%及49%。系统进化树分析表明,瓦氏黄颡鱼TLR2与斑点叉尾聚为一支。q RT-PCR分析表明,TLR2 m RNA在检测的组织中均有表达,且在头肾和脾脏中表达水平显著高于其他组织(P0.05)。嗜水气单胞菌感染能显著上调瓦氏黄颡鱼肝脏、头肾及脾脏中TLR2 m RNA表达(P0.05),分别在24h、48h及12h达到最大值。头肾中的TLR 2信号通路下游的髓样分化因子、半胱氨酸蛋白酶8、核转录因子kappa B、肿瘤坏死因子α、白细胞介素1βm RNA均显著上升(P0.05),分别在24h、12h、48h,48h和48h达到最大值。结果表明,嗜水气单胞菌感染激活了TLR2信号通路,通过上调表达,肿瘤坏死因子α,白细胞介素1β等。本研究表明,TLR2在瓦氏黄颡鱼抵御嗜水气单胞菌侵染的过程中发挥了重要的免疫作用。  相似文献   

The 1‐million‐year‐old quartz assemblage from Pont‐de‐Lavaud (Centre,France) in the European context          下载免费PDF全文

Jackie Despriée  Marie‐Hélène Moncel  Marta Arzarello  Gilles Courcimault  Pierre Voinchet  Jean‐Jacques Bahain  Christophe Falguères 《第四纪科学杂志》2018,33(6):639-661

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基于高斯两步移动搜寻法的村镇避难场所可达性研究     

但文羽  肖映辉  胡周灵  詹庆明 《地球信息科学学报》2018,20(10):1412-1421

避难场所可达性是指避难场所与受灾居民之间的通达性,即灾害发生时,避难人员通过避难疏散通道到达避难场所的难易程度,是评价避难场所布局合理性的重要指标。本研究结合高斯两步移动搜寻法和网络分析法,从供需双向出发对村镇地区避难场所进行可达性分析。2种方法的结合,既充分考虑了需求点与设施点的相互作用,又充分考虑了设施点的吸引力随距离的衰减关系,并基于实际道路,以步行疏散时间作为搜索半径,降低了传统研究中因忽略供需间相互作用及实际距离而引起的可达性结果的误差。最后,以神农架松柏镇区为例,验证该方法的实际应用价值。结果表明,该方法能够有效测定村镇地区避难场所的空间可达性,同时,基于GIS软件可以直观地揭示研究区域内避难场所可达性空间分布差异。该方法可为制定科学的村镇防灾减灾规划提供方法支持。  相似文献   

Modelling the effect of changing precipitation inputs on deep soil water utilization          下载免费PDF全文

Ji Qi  Daniel Markewitz  David Radcliffe 《水文研究》2018,32(5):672-686

Forests in the Southeastern United States are predicted to experience future changes in seasonal patterns of precipitation inputs as well as more variable precipitation events. These climate change‐induced alterations could increase drought and lower soil water availability. Drought could alter rooting patterns and increase the importance of deep roots that access subsurface water resources. To address plant response to drought in both deep rooting and soil water utilization as well as soil drainage, we utilize a throughfall reduction experiment in a loblolly pine plantation of the Southeastern United States to calibrate and validate a hydrological model. The model was accurately calibrated against field measured soil moisture data under ambient rainfall and validated using 30% throughfall reduction data. Using this model, we then tested these scenarios: (a) evenly reduced precipitation; (b) less precipitation in summer, more in winter; (c) same total amount of precipitation with less frequent but heavier storms; and (d) shallower rooting depth under the above 3 scenarios. When less precipitation was received, drainage decreased proportionally much faster than evapotranspiration implying plants will acquire water first to the detriment of drainage. When precipitation was reduced by more than 30%, plants relied on stored soil water to satisfy evapotranspiration suggesting 30% may be a threshold that if sustained over the long term would deplete plant available soil water. Under the third scenario, evapotranspiration and drainage decreased, whereas surface run‐off increased. Changes in root biomass measured before and 4 years after the throughfall reduction experiment were not detected among treatments. Model simulations, however, indicated gains in evapotranspiration with deeper roots under evenly reduced precipitation and seasonal precipitation redistribution scenarios but not when precipitation frequency was adjusted. Deep soil and deep rooting can provide an important buffer capacity when precipitation alone cannot satisfy the evapotranspirational demand of forests. How this buffering capacity will persist in the face of changing precipitation inputs, however, will depend less on seasonal redistribution than on the magnitude of reductions and changes in rainfall frequency.  相似文献   

Properties of geogrid-reinforced marine slope due to the groundwater level changes     

Ramin Vali  Jie Li  Gholamreza Shams  Pieter van Gelder 《Marine Georesources & Geotechnology》2018,36(6):735-748

Evaluation of slope stability, especially in the absence of a proper bed such as marine soils, is one of the most important issues in geotechnical engineering. Using geogrid layers to enhance the strength and stability of embankments is regarded as a commendable stabilization method. On the other hand, groundwater level erratically fluctuates in coastal areas. Therefore, the aim of this research is to study the effects of groundwater level changes on stability of a geogrid-reinforced slope on loose marine soils in Qeshm Island, Iran. At first, geotechnical properties of the site were obtained by comprehensive series of geotechnical laboratory and in situ tests. Then, by simultaneous changes of groundwater level and several parameters such as embankment slope, loading, geogrid length, geogrid number, and tensile strength of geogrid, different characteristics such as embankment safety factor (SF), vertical and horizontal displacements at embankment top and embankment base were studied. It was observed that groundwater level had significant effects on behavior of the embankment. For most of the observations, by decreasing the groundwater level, the displacements decreased and consequently safety factor increased. Increasing the length, number, and tensile strength of geogrid led to the reduction of displacements and an increase in the safety factor.  相似文献   

北斗在南/北极地区的基本定位性能评估     

徐炜  贾雪  乔方  刘扬  严超  王涛 《大地测量与地球动力学》2018,38(12):1268-1273

基于BDS、GPS系统的星座结构，对当前的BDS二代导航系统（BD2）、全部建成后的BDS系统在极地科考站（黄河站、昆仑站、中山站、长城站）和北极圈的可见卫星数、DOP值、定位精度等进行评估，并将建成后的BDS、GPS及其组合系统在南/北极的基本定位性能进行对比分析。仿真结果表明，当前的BD2只实现了极地的部分覆盖，对极地提供导航定位的能力有限，大范围内的定位精度大于30.0 m； BDS在极地的定位精度将与GPS相当，可见卫星数可达13颗左右，PDOP值优于1.6，定位精度优于8.0 m；GPS/BDS组合后在极地的PDOP值优于1.4，定位精度优于6.0 m。
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典型湖泊天然有机质与四环素的相互作用     

曹驰程  王友权  章奇  张晖 《湖泊科学》2018,30(4):1004-1011

采用荧光滴定法研究四环素(tetracycline,TC)与太湖溶解性有机质(dissolved organic matter,DOM)和玄武湖DOM的相互作用.三维荧光光谱结合平行因子分析显示,2个湖泊的DOM含有3个荧光组分:类富里酸组分C1、类色氨酸组分C2和类络氨酸组分C3.其中C2的荧光强度远高于C1和C3,是DOM的主要荧光组成.3个荧光组分与TC发生了不同程度的静态猝灭,特别当TC浓度为45.5μmol/L时,类蛋白组分的荧光强度完全被猝灭(100%),并且猝灭作用改变了DOM分子的微环境极性.同步荧光光谱联合二维相关图谱进一步表明类色氨酸组分优先和TC发生猝灭作用,其次为类络氨酸组分和类富里酸组分.Ryan-Weber方程适于拟合DOM与TC的猝灭过程,2个湖泊的DOM中3个荧光组分的络合常数lg K值范围为5.05~5.85,大小顺序为C2C3C1.因此,类蛋白组分为主的DOM对TC的络合作用大于类腐殖组分为主的DOM,影响抗生素在湖泊水体中的生物有效性和生态毒性.  相似文献   

鄱阳湖湿地典型植被群落地下水—土壤—植被—大气系统界面水分通量及水源组成     

许秀丽  李云良  谭志强  张奇 《湖泊科学》2018,30(5):1351-1367

地下水-土壤-植被-大气系统(GSPAC)界面水分传输是湿地生态水文过程研究的关键.本文选取鄱阳湖湿地高位滩地的2种典型植被群落:茵陈蒿(Artemisia capillaris)和芦苇(Phragmites australis)群落为研究对象,运用HYDRUS-1D垂向一维数值模拟,量化了湿地GSPAC系统界面水分通量,阐明了典型丰水年(2012年)和枯水年(2013年)鄱阳湖湿地植被群落的蒸腾用水规律和水源组成.结果表明:(1)茵陈蒿和芦苇群落土壤-大气界面的年降水入渗量为1570~1600 mm,主要集中在雨季4-6月,占年总量的60%;植物-大气界面的年蒸腾总量分别为346~470 mm和926~1057 mm,其中7-8月植被生长旺季最大,占年总量的40%~46%;地下水-根区土壤界面的向上补给水量受不同水文年水位变化的影响显著,地下水年补给量分别为15~513 mm和277~616 mm,主要发生在蒸散发作用强烈和地下水埋深较浅的时段.(2)植被蒸腾用水分为生长初期(4-6月)和生长旺季(7-10月)2个阶段,丰水年植被的整个生长期蒸腾用水充足,枯水年植被生长旺季的蒸腾用水受到严重水分胁迫,实际蒸腾量仅为潜在蒸腾量的一半左右.(3)不同水文年湿地植被生长旺季的水源贡献不同:丰水年茵陈蒿群落以地下水补给为主,芦苇群落以湖水和地下水补给为主;枯水年茵陈蒿群落以降水和前期土壤水储量为主,芦苇群落以地下水补给为主.本研究结果有助于揭示湿地植被的水分利用策略,为阐明湖泊水情变化与植被演替的作用机理提供参考依据.  相似文献   

Variations in glacier volume and snow cover and their impact on lake storage in the Paiku Co Basin,in the Central Himalayas     

Xingxing Zhang  Rui Wang  Zhijun Yao  Zhaofei Liu 《水文研究》2020,34(8):1920-1933

Glaciers and snow cover are important constituents of the surface of the Tibetan Plateau. The responses of these phenomena to global environmental changes are sensitive, rapid and intensive due to the high altitudes and arid cold climate of the Tibetan Plateau. Based on multisource remote sensing data, including Landsat images, MOD10A2 snow product, ICESat, Cryosat-2 altimetry data and long-term ground climate observations, we analysed the dynamic changes of glaciers, snow melting and lake in the Paiku Co basin using extraction methods for glaciers and lake, the degree-day model and the ice and lake volume method. The interaction among the climate, ice-snow and the hydrological elements in Paiku Co is revealed. From 2000 to 2018, the basin tended to be drier, and rainfall decreased at a rate of −3.07 mm/a. The seasonal temperature difference in the basin increased, the maximum temperature increased at a rate of 0.02°C/a and the minimum temperature decreased at a rate of −0.06°C/a, which accelerated the melting from glaciers and snow at rates of 0.55 × 10⁷ m³/a and 0.29 × 10⁷ m³/a, respectively. The rate of contribution to the lake from rainfall, snow and glacier melted water was 55.6, 27.7 and 16.7%, respectively. In the past 18 years, the warmer and drier climate has caused the lake to shrink. The water level of the lake continued to decline at a rate of −0.02 m/a, and the lake water volume decreased by 4.85 × 10⁸ m³ at a rate of −0.27 × 10⁸ m³/a from 2000 to 2018. This evaluation is important for understanding how the snow and ice melting in the central Himalayas affect the regional water cycle.  相似文献   

A-FPN算法及其在遥感图像船舶检测中的应用     

于野  艾华  贺小军  于树海  钟兴  朱瑞飞 《遥感学报》2020,24(2):107-115

光学遥感图像船舶检测主要面临两个挑战:光学遥感图像背景复杂,船舶检测易受海浪、云雾及陆地建筑等多方面干扰;遥感图像分辨率低,船舶目标小,对于其分类与定位带来很大困难;针对上述问题,在FPN的基础上,提出一种融入显著性特征的卷积神经网络模型A-FPN (Attention-Based Feature Pyramid Networks)。首先,利用卷积提取图像特征金字塔;然后,利用顶层金字塔逐级构建显著特征层,抑制背景信息,通过金字塔顶层的细粒度特征提高浅层特征的表达能力,构建自上而下的多级显著特征映射结构;最后利用Softmax分类器进行多层级船舶检测。A-FPN模型利用显著性机制引导不同感受下的特征进行融合,提高了模型的分辨能力,对遥感图像处理领域具有重要应用价值。实验阶段,利用公开的遥感目标检测数据集NWPU VHR-10中的船舶样本进行测试,准确率为92.8%,表明A-FPN模型适用于遥感图像船舶检测。  相似文献