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Possible mediatory roles of heat acclimation and salicylic acid in protecting the sporophyte of marine macroalga Laminaria japonica (Phaeophyceae) from heat stress were studied. Heat stress resulted in oxidative injury in the kelp blades. Under heat stress
significant accumulation of hydrogen peroxide (H2O2) and malonaldehyde (MDA), a membrane lipid peroxidation product, and a drastic decrease in chlorophyll a content were recorded. Activity of the enzymatic antioxidant system was drastically affected by heat stress. The activity
of superoxide dismutase (SOD) was significantly increased while peroxidase (POD), catalase (CAT) and glutathione peroxidase
(GPX) were greatly inhibited and, simultaneously, phenylalanine ammonia-lyase was activated while polyphenol oxidase (PPO)
was inhibited. Both heat acclimation pretreatment and exogenous application of salicylic acid alleviated oxidative damage
in kelp blades. Blades receiving heat acclimation pretreatment and exogenous salicylic acid prior to heat stress exhibited
a reduced increase in H2O2 and MDA content, and a lower reduction in chlorophyll a content. Pretreatment with heat acclimation and salicylic acid elevated activities of SOD, POD, CAT, GPX and PPO. Considering
these results collectively, we speculate that the inhibition of antioxidant enzymes is a possible cause of the heat-stress-induced
oxidative stress in L. japonica, and enhanced thermotolerance may be associated, at least in part, with the elevated activity of the enzymatic antioxidant
system. 相似文献
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实验室条件下,研究不同抗生素胁迫对小球藻(Chlorella vulgarisBeij.)、金藻8701(Isochrysis galbana Parke8701.)和小新月菱形藻(Nitzschia closteriumEhr.)生长的影响。结果表明:1)低于100μg.mL-1的氯霉素作用下,小球藻和金藻8701叶绿素a含量变化与对照组无明显差异;小新月菱形藻叶绿素a含量显著降低。2)不同浓度梯度的遗传霉素(G418),均显著抑制3种微藻叶绿素a含量的增加。3)青霉素浓度低于100μg.mL-1时,能够促进3种藻叶绿素a的增加,但不同微藻间叶绿素a含量的相对增长率存在明显差异。试验结果可为微藻的基因工程选择标记和无菌培养体系的建立提供参考。 相似文献
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双齿围沙蚕(Perinereis aibuhitensis)亲体为2010 年半人工育苗的苗种, 人工培育至异沙蚕体后进行集中催产、人工授精, 受精卵经过消毒后, 孵化至囊胚期, 收集入水泥池进行中间培育, 经3 d 孵化至3 刚节幼体再次收集, 放入土池进行幼虫培育。卵子受精后, 定期(早期每隔1 h, 囊胚期后每隔3 h)取受精卵, 置于OLYMPUS-CX31 显微镜进行活体观察并进行拍照。结果表明, 在双齿围沙蚕的胚胎发育过程中, 出现了与已有结论不符的特殊现象, 例如油球与胚胎发育的关系, 胚胎的高温孵化。而且,在孵化期, 一种寄生纤毛虫导致沙蚕胚胎及出膜3 刚节疣足幼体的大量死亡, 其病害机理有待进一步研究。 相似文献
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We investigated the effect of CO2 enrichment and initial inoculum density on competition between Skeletonema costatum and Heterosigma akashiwo, two common algae seen in algal blooms. The initial inoculum density (0.2×104, 0.4×104, 0.8×104 cells/ml) had a significant effect on population growth and competition between H. akashiwo and S. costatum. The time required for population growth to reach the exponential growth phase and stationary phase decreased significantly
as the initial density increased. When the two species were cultured together, S. costatum tended to outcompete H. akashiwo, especially when present at higher initial ratios. CO2 enrichment (5 000 μl/L CO2) increased the maximum population density and carrying capacity of H. akashiwo but decreased these measures for S. costatum. Thus, CO2 enrichment favored the growth of H. akashiwo over S. costatum. 相似文献
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实验生态条件下研究十溴联苯醚(BDE-209)的急性毒性胁迫对褶皱臂尾轮虫(Branchionus plicatilis)运动行为与摄食行为的影响,探讨可用以表征胁迫-响应的生物标志物。结果表明:(1)BDE-209胁迫24h显著性增加了褶皱臂尾轮虫的运动抑制率,由此得到24h半数有效抑制浓度(24h-EC_(50))为9.084mg/L,但未达到半数致死作用;(2)当分别以四种海洋微藻:小球藻(Chlorella vulgaris)、青岛大扁藻(Platymonas helgolandica)、等鞭金藻8701(Isochrysis galbana)和小新月菱形藻(Cylindrotheca closterium)为单一饵料来源时,褶皱臂尾轮虫对四种饵料单胞藻的滤水率(F)和摄食率(G)均随着胁迫时间和胁迫浓度的增加而不断下降,呈现出明显的浓度依赖性,说明BDE-209胁迫能显著抑制褶皱臂尾轮虫的摄食作用。(3)将其中三种微藻等生物量混合投喂时,褶皱臂尾轮虫对三种微藻的滤水率和摄食率与单一饵料投喂条件相比均发生变化,且对各种微藻的选食率(Sr)和摄食总生物量均随着胁迫时间和浓度的增加呈下降趋势,选择性指数(Si)发生变化,对小球藻的摄食选择性最高。滤水率的变化较其它指标更敏感,可用以指示浮游动物在个体水平上响应外源环境变化的标志物。结果表明,BDE-209胁迫能有效改变褶皱臂尾轮虫的摄食与选食行为,且其最适宜的饵料是小球藻。 相似文献
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采用单养和混养的方法,在实验条件下研究了东海原甲藻对中华哲水蚤摄食和消化酶活性的影响.结果表明:(1)中华哲水蚤对东海原甲藻存在一定摄食行为,藻类密度对摄食率有明显的影响.实验密度下,中华哲水蚤对东海原甲藻的最大摄食率为930cells/(ind·h).滤水率随着藻密度的增加而呈单一性的下降;(2)混养条件下,中华哲水蚤对金藻和东海原甲藻的摄食率均较单养时下降,滤水率的变化与摄食率相似;(3)不同藻密度下,昆布多糖酶活性都明显高于麦芽糖酶和纤维二糖酶的活性,而麦芽糖酶活性又稍高于纤维二糖酶的活性.与金藻相比,东海原甲藻实验组中华哲水蚤3种消化酶活性明显升高(P<0.05). 相似文献