AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究   总被引：24，自引：3，他引：21  

张俊彬  黄良民  陈真然 《海洋学报》2005,27(2):165-171

以南沙群岛采获的勒氏笛鲷(Lutjanus russelli)、黄笛鲷(Lutjanus lutjanus)、红笛鲷(Lut janus sanguineus)、紫红笛鲷(Lutjanus argentimaculatus)、金焰笛鲷(Lutjanus fulviflamma)、线纹笛鲷(Lutjanus lineolatus)、画眉笛鲷(Lutjanus vitta)、马拉巴笛鲷(Lutjanus malabarius)、驼背笛鲷(Lutjanus gibbus)、约氏笛鲷(Lutjanus johni)、金带笛鲷(Lutjanus vaigiensis)11种笛鲷属的后期仔鱼为材料, 进行了基因组DNA的AFLP(扩增片段长度多态性)研究,通过笛鲷仔鱼和成鱼的AFLP电泳图谱的比较分析,将这11种笛鲷属的仔鱼成功分开.研究表明,最适合的AFLP引物组合是E+AGC/M+CAA,由这11种笛鲷共扩增出132 AFLP位点,每种笛鲷的AFLP条带数为43~69,在这11种笛鲷中,共享的AFLP条带数仅为7,同一种笛鲷仔鱼和成鱼AFLP遗传相似度在90%以上.根据这11种笛鲷的Nei遗传距离对Neighboring系统进行进化分析,并且与传统的笛鲷形态分类进行了比较.分子生物学可以阐释笛鲷属鱼类的系统进化和遗传亲缘关系,对传统形态分类学加以完善和补充.  相似文献   

An endemic freshwater mussel species from the Orontes River basin in Turkey and Syria represents duck mussel’s intraspecific lineage: Implications for conservation     

《Limnologica》2020

Amik Lake or, historically, Lake of Antioch, was a large freshwater body in the lower Orontes River basin (Hatay Province, Turkey) that was drained in the 1940s–1970s. Several endemic animal species were described from this lake, including the freshwater mussel Anodonta pseudodopsis Locard, 1883 (Bivalvia: Unionidae) characterized by a large rounded shell covered by a peculiar yellow or yellowish-brown periostracum. Molecular analyses of topotypes of this nominal taxon collected from the former lake’s tributaries in the Amik Plain indicate that it is an intra-specific lineage of the widespread Anodonta anatina (Linnaeus, 1758) based on the mitochondrial COI and 16S rRNA, and the nuclear 28S rRNA gene fragments. Geometric morphometric analyses using the lectotype and topotypes of Anodonta pseudodopsis support our DNA-based hypothesis on the status of this nominal taxon. A new synonymy is provided as follows: Anodonta anatina = Anodonta pseudodopsis syn. nov. The syntype of Anodonta pseudodopsis SMF 5129 “See von Antiochia” (Senckenberg Research Institute and Natural History Museum, Frankfurt, Germany) is designated here to be the lectotype of this nominal taxon. Finally, we conclude that Anodonta anatina range covers the Orontes River basin in Turkey and Syria and the Nahr al-Kabir al-Shamali River in the Latakia Governorate of Syria. This intraspecific lineage of Anodonta anatina and other freshwater mussels of the Middle East are highly threatened due to multiple anthropogenic impacts and must be a focus of international conservation efforts. The Karasu River in eastern Turkey hosts viable populations of all freshwater mussel species of the Orontes’s fauna and can be considered one of the most important water bodies for the conservation of these imperiled animals in the region.  相似文献   

Synonymy and circum‐tropical biogeography of the Devonian calcareous alga Zeapora Penecke, 1894 (Chlorophyta,Bryopsidales)          下载免费PDF全文

Bernhard Hubmann  Markus Reuter 《Island Arc》2017,26(3)

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Alentiana palinpoda,a new commensal polynoid species from a seamount in the Northwest Pacific Ocean     

Yueyun Wang  Hong Cheng  Chunsheng Wang 《海洋学报(英文版)》2021,40(12):12-19

Alentiana has only one member, A. aurantiaca (Verrill, 1885), commensal with sea anemone (Bolocera tuediae). Here, Alentiana palinpoda sp. nov., a second member in the genus Alentiana (subfamily Lepidastheniinae), a new commensal polynoid is described, based on a specimen collected from a deep-water seamount in the Northwest Pacific Ocean. The new species is characterized by large elytra that completely cover the dorsum and are present in every third segment in the posterior regions. Neuropodia are truncated, with two types of neurochaetae;supraacicular chaetae toothed on one side with a slightly inflated end, and subacicular chaetae that are heavy and smooth; the prechaetal lobe is larger than the postchaetal lobe, with the ventral part rolling upward and  相似文献   

Ophiuroid fauna of cobalt-rich crust seamounts in the Northwest Pacific Ocean     

Wanying Chen  Jieying Na  Chengcheng Shen  Ruiyan Zhang  Bo Lu  Hong Cheng  Chunsheng Wang  Dongsheng Zhang 《海洋学报(英文版)》2021,40(12):55-78

Seamounts are vulnerable ecosystems in the deep sea and can be heavily impacted by human activities, such as bottom fishing and deep-sea mining. The species composition and distribution patterns of benthic fauna is key information for the designation of marine protected areas and environmental management plans. Three contracts for cobalt-rich crust exploration have been granted to China, Japan and Korea in the Northwest Pacific Ocean by the International Seabed Authority. However, our knowledge of benthic biodiversity in this area is extremely insufficient. During 2013–2020, eight Chinese Ocean Mineral Resources R&D Association (COMRA) cruises were conducted to investigate the benthic assemblages of nine seamounts in this region. In this study, 191 ophiuroids collected from seamounts in the Northwest Pacific were identified into 29 species in 11 families. Ophiacanthidae and Euryalidae were the two most dominant families with 12 and 6 species, respectively. Ophiotomidae and Ophiopyrgidae were represented by two species each, while seven families were represented by only one species. Four species were widely distributed among 4–5 seamounts, and 17 species were found only at a single site. An integrated regional taxonomic dataset of Ophiuroidea was generated and analyzed. A total of 23 and 14 species were obtained from the Magellan Seamount Chain (MSC) and the Marcus-Wake seamounts (MWS), respectively, with 8 species shared between the two seamount groups. The individual-based rarefaction curves did not reach an asymptote, suggesting that the sampling effort was inadequate for either the entire region or each single seamount. Most species distributed in a narrow depth range, and the species composition was different between water depths above and below 2000 m. Our results greatly improve the understanding of megafaunal biodiversity from seamounts in the Northwest Pacific Ocean, and highlight the necessity of further surveys to provide more robust information for environmental protection and management in this region.  相似文献   

A new species of the glass sponge genus Walteria (Hexactinell-ida: Lyssacinosida: Euplectellidae) from northwestern Pacific seamounts,providing a biogenic microhabitat in the deep sea     

Chengcheng Shen  Hong Cheng  Dongsheng Zhang  Bo Lu  Chunsheng Wang 《海洋学报(英文版)》2021,40(12):39-49

We report on a hexactinellid sponge new to science, Walteria demeterae sp. nov., which was collected from the northwestern Pacific seamounts at depths of 1271–1703 m. Its tubular and basiphytous body, extensive lateral processes, numerous oval lateral oscula which are irregularly situated in the body wall, the presence of microscleres with oxyoidal, discoidal and onychoidal outer ends, and the absence of anchorate discohexasters, indicate it belongs to the genus Walteria of family Euplectellidae, which is also supported by molecular phylogenetic evidence from 18S, 28S, 16S rRNA and cytochrome c oxidase subunit I (COI) gene sequences. The unique morphotype, which is structured by a thin and rigid framework of body wall and lateral processes consisting of diactins, characterizes it as a new species. Local aggregations of individuals of this new species coupled with their associated macrofauna in the Suda Seamount are reported, highlighting its functional significance in providing biogenic microhabitats in the deep sea.  相似文献   

中国东海颈栉虫属(多毛纲:双栉虫科)一新记录种     

隋吉星  李新正 《海洋学报(英文版)》2018,37(10):145-147

本文报道和描述了中国东海双栉虫科颈栉虫属一新记录种，沃氏颈栉虫。本文标本与沃氏颈栉虫的原始描述非常吻合，包括具有两对鳃，胸齿片刚节具有两对加长的腹疣足和具有12个腹部齿片刚节。本文的标本具有长的须状的鳃，可延伸到胸部第9刚节，这在原始描述中没有提及。本文首次在中国海域记录本种。  相似文献   

四种贝类和鱼类外寄生车轮虫的国内新纪录     

詹子锋  ZENG Hong  LIN Nengfeng  徐奎栋 《海洋学报(英文版)》2018,37(10):91-97

车轮虫是一类主要寄生于贝类和鱼类体表的缘毛类纤毛虫原生动物。本文对分别寄生于海洋贝类－－虾夷扇贝Mizuhopecten yessoensis，半咸水鱼类－－鲻鱼Mugil cephalus和双色鳗鲡Anguilla bicolor bicolor，以及淡水鱼类－－光唇鱼Acrossocheilus fasciatus鳃表和体表的虾夷扇贝车轮虫Trichodina pectenis，亚卓车轮虫T.jadranica，急尖车轮虫T.acuta和喙状车轮虫T.rostrata，进行了详细的分类学研究。本文第一次报道了虾夷扇贝车轮虫的活体和蛋白银染色后特征，该虫体也是国内新记录种。其它三个车轮虫物种感染的鱼类，均属新纪录宿主。同时，也报道了这四种车轮虫对宿主的感染率和感染强度。  相似文献   

浮游软体动物长角螺属(Clio)种类的分类学和谱系地理学     

高阳  李海涛  姜重臣  陈志云  李宏俊 《海洋学报》2020,42(2):96-105

许多终生浮游软体动物接近全球分布或呈环球分布,是海洋酸化和谱系地理学研究的良好材料。本文以西北太平洋和北印度洋的长角螺属(Clio)种类为材料,通过测定其线粒体COI基因(mtCOI,55条)和核18S rRNA基因(9条)序列,结合数据库中已有的序列,对该属进行了分类学和谱系地理学研究。结果表明,矛头长角螺(C. pyramidata)和尖棘长角螺(C. cuspidata)在mtCOI基因系统树中均形成4个明显分化的谱系分支,分别为支系A–D和支系E–H。矛头长角螺的支系A为全球分布,中国海及邻近海域可能仅有支系A的存在,支系B、C和D分布于特定海域。尖棘长角螺也存在明显的谱系地理结构,西北太平洋北赤道流南北两侧存在2个不同的支系,分布于吕宋海峡的支系E为一新的谱系分支。各支系内mtCOI基因的K2P遗传距离在0~0.026之间,支系间的遗传距离在0.031~0.089之间。膨凸长角螺(C. convexa)和曲形长角螺(C. recurva)没有明显的地理遗传分化。18S rRNA基因支持支系D为独立种,但不支持其他支系的划分。矛头长角螺和尖棘长角螺内部可能存在隐存多样性。洋流可能会成为物种扩布和基因交流的障碍。  相似文献   

中国沿海球形棕囊藻(Phaeocystis globosa)的分类、分布及其藻华     

沈萍萍  齐雨藻  欧林坚 《海洋科学》2018,42(10):146-162

球形棕囊藻(Phaeocystis globosa Scherffel)是全球海洋广泛分布的有害藻华种类。1997年10月,中国东南沿海首次暴发了此种藻类的大规模藻华,其后陆续在福建、广东、广西、海南、河北及天津等省市沿海暴发多起同种藻华。中国近海的球形棕囊藻藻华呈现两大独有的特点,即藻类囊体较大(可达3厘米),以及藻华可毒害养殖业。历经20多年,球形棕囊藻在中国沿海已从一个"藻华新记录种"变成了"藻华常见种"。值得注意的是, 2014年以来,广西北部湾海域棕囊藻藻华肆虐,威胁核电冷源安全,受到了社会高度关注,也对球形棕囊藻藻华的研究提出了新的挑战与要求。针对这一生态灾害的最新发展趋势,本文总结了20年来中国球形棕囊藻及其藻华灾害的发生与发展状况,分别就棕囊藻的分类、生活史特征、营养特性、藻华形成的环境驱动因素、生态毒理等诸多方面开展简要综述,冀望为棕囊藻藻华的研究及防治提供基础资料及思路。  相似文献