中国花鲈细胞色素b基因片段的序列研究(英文)     

高天翔  张秀梅  陈大刚  张美昭  任一平  张亚平 《中国海洋大学学报(自然科学版)》2001,31(2)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。  相似文献   

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

基于案例推理的海洋涡旋特征信息空间相似性研究   总被引：5，自引：0，他引：5  

杜云艳  苏奋振  仉天宇  杨晓梅  周成虎 《热带海洋学报》2005,24(3):1-9

目前对海洋涡旋的分析主要采用水团、近海海流分析和数值模拟方法，这些方法结合不同的区域资料已经有过很多成功的研究。但面对日益增长的多源、大面积海洋遥感数据，在快速自动化提取海洋涡旋的空间信息方面还存在很多难题。因此该文针对海洋涡旋空间信息的快速定量分析问题，提出了基于案例推理(Case-Based Reasoning，CBR)的海洋涡旋空间相似性研究方法。该方法首先采用多源数据(遥感数据、遥感反演数据、观测数据等)进行历史涡旋案例定量分析，并构建基于CBR的海洋涡旋案例表达模型和历史案例库；继而采用“基于重心矢径的序列分析方法”进行历史涡旋空间相似性分析，并结合典型的海洋涡旋区域给出具体实例。研究结果表明该方法是可行的。  相似文献   

西沙礁序列的磁性地层学研究   总被引：3，自引：0，他引：3  

张明书  周墨清 《海洋地质与第四纪地质》1996,16(3):61-65

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。  相似文献   

三株赤潮硅藻5.8SrDNA及转录间隔区(ITS)的克隆及序列分析     

张宝玉  王广策  吕颂辉  齐雨藻  邹景忠  曾呈奎 《海洋学报》2006,28(1):111-117

对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位.  相似文献   

时间序列分析的一种新方法     

潘增宪 《海洋信息》1993,(11):20-21

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Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献   

鹿心社对灾后重建测绘保障工作提出要求     

王增宁 《中国测绘》2008,(3):8-8

5月25日下午,国家测绘局召开局长办公会,研究部署继续全力做好抗震救灾、灾后重建测绘保障服务工作。国土资源部副部长、国家测绘局局长鹿心社在会上充分肯定了国家测绘局及所属各单位为抗震救灾提供的测绘保障服务。  相似文献   

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China     

蔡立国  周雁  白振瑞 《中国地质大学学报(英文版)》2008,19(6):645-655

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.  相似文献   

BP神经网络模型在澜沧江流域径流量模拟中的应用     

LIU Gang    LIU Ji-ping  ZHAO Rong  ZHANG Qing-pu 《测绘学院学报》2008,(4)

河川径流等水文时间序列属于复杂的非线性系统,使用回归分析等传统的分析方法,难以获取和描述其内在关联和变化规律。利用现有的相关站点的径流量历史数据和输沙量、降水量数据,在进行规格化处理和主成分分析的基础上,利用三层BP人工神经网络模型,对澜沧江流域上游昌都站径流量与各关联因子之间复杂的非线性映射关系进行模拟,采用拟牛顿算法对模型进行训练,模拟结果达到期望精度要求,并利用1982年~1985年实测数据进行模型验证。结果证明利用BP神经网络模型对澜沧江流域站点的月径流量序列进行模拟、预测和数据补缺处理具有可行性。  相似文献