海洋解淀粉芽孢杆菌(Bacillus amyloliquefacien)产抗生素Macrolactin A的碳源优化     

侯东园  吴祖芳  张鑫 《海洋与湖沼》2015,46(5):1071-1077

为提高一株海洋解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ESB-2生产大环内酯类抗生素Macrolactin A的能力,利用大孔树脂对发酵液进行粗提取,利用高速逆流色谱法,高效、快速分离制备出高纯度的Macrolactin A。采用高效液相色谱法检测不同碳源对Macrolactin A产量的影响,且在优化基础上采用摇瓶发酵得到海洋解淀粉芽孢杆菌的发酵过程曲线。结果表明:高速逆流色谱法能够高效快速的制备出Macrolactin A纯品,纯度达到95%以上,明显优于高效液相色谱;添加葡萄糖、蔗糖和麦芽糖均有利于Macrolactin A的生成,麦芽糖为最佳碳源,最佳浓度为1%,其产量达到18.5mg/L,是优化前的2倍多。本研究可为提高Macrolactin A的产量、解决其药源问题、实现规模化生产打下了良好的基础。  相似文献   

High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A          下载免费PDF全文

Ornella Affinito  Nikos Andreakis  Luigi Caputi  Rita Marino  Raimondo Pannone  Paolo Sordino  Gabriele Procaccini 《Marine Ecology》2015,36(4):1230-1243

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.  相似文献   

基于HJ-1A高光谱的黄河口碱蓬和柽柳盖度反演模型研究   总被引：3，自引：2，他引：1  

任广波  张杰  马毅 《海洋学报》2015,37(9):51-58

碱蓬和柽柳是黄河口湿地典型的盐生植物类型,是多种保护珍禽的主要栖息地,具有景观尺度较小、分布广且多混生的特点。应用覆盖黄河口北部潮滩的HJ-1A高光谱遥感影像,基于现场测量的端元光谱和从遥感影像中使用顺序最大角凸锥法(SAMCC)自动提取的端元光谱,应用线性光谱分解法(LSU)、正交子空间投影法(OSP)、匹配滤波法(MF)、最小能量约束法(CEM)和自适应一致估计法(ACE)5种不同光谱解混方法进行混合像元光谱解混,对比两种方法得到的端元光谱分别对碱蓬和柽柳盖度的反演能力,并给出相应的反演模型。结果显示:(1)现场测量端元光谱取得了较好的碱蓬和柽柳盖度反演结果,其中应用LSU方法的光谱解混结果与现场测量盖度的决定系数对于碱蓬和柽柳分别达到了0.88和0.95;(2)两种端元获取方式的光谱解混结果中,LSU和OSP方法均获得了较高的相关性,ACE解混方法的相关性都最低;(3)SAMCC方法提取端元光谱对柽柳的分解结果与现场测量盖度的相关性远高于碱蓬。  相似文献   

Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding     

LEE Hyung Woo  KIM Myung Sook 《海洋学报(英文版)》2015,34(4):114-124

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU(tuf A) is considered as maker to perform DNA barcoding of green algal species than rbc L gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tuf A marker, we newly generated fifty-five tuf A barcodes to resolve eight species. Tuf A marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbc L exon 1,3.5%–11.5%. Molecular analysis of rbc L exon 1 sequences of Codium revealed eight distinct species like tuf A analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tuf A marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbc L analysis alone. The complement of tuf A barcoding and rbc L analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.  相似文献   

HY-2A卫星雷达高度计数据的全球统计评价及质量分析   总被引：6，自引：4，他引：2  

彭海龙  林明森  穆博  周武 《海洋学报》2015,37(7):54-66

自HY-2A卫星发射以来,针对HY-2A卫星雷达高度计产品的交叉定标、真实性检验及质量评估工作一直在持续开展。本文主要以HY-2A卫星高度计第44周期的IGDR产品数据为例,通过使用全球分布图、二维直方图和每日均值统计的方法完成了与Jason-2IGDR产品的比对验证,同时对主要环境校正参数及地球物理产品的数据质量稳定性进行了分析,结果显示高度计产品数据质量较稳定,此外利用HY-2A卫星升降轨交叉点海面高度差、与Jason-2卫星交叉点海面高度差以及沿轨海平面异常数据分析的方法进行了HY-2A卫星高度计观测系统的性能评估,结果显示,HY-2A卫星海面高度精度约为7.48cm,精度接近Jason-2,能满足海洋应用与科学研究的需要。  相似文献   

利用GOCE模拟观测反演重力场的Torus法   总被引：1，自引：1，他引：0  

刘焕玲  汉江  徐新禹  朱广彬 《测绘学报》2015,44(9):965-972

在介绍Torus方法反演地球重力场模型的基本原理和方法的基础上,基于圆环面上均匀分布的卫星引力梯度模拟观测值解算了200阶次的地球重力场模型,在无误差情况下,Torus方法解算模型的阶误差RMS小于10-16,验证了该方法的严密性。利用61dGOCE卫星轨道上无误差的模拟引力梯度观测值解算了200阶次的地球重力场模型,分析了格网化误差、极空白对解算精度的影响,迭代3次后,在不考虑低次系数情况下,模型的大地水准面阶误差和累积误差均较小,最大值仅为0.022mm和0.099mm。在沿轨卫星引力梯度模拟数据中加入5mE/Hz1/2的白噪声,基于Torus方法和空域最小二乘法解算了200阶次的地球重力场模型,Torus方法的精度略低于空域最小二乘法的精度,在不考虑低次项的情况下,两种方法解算模型的大地水准面阶误差最大值分别为1.58cm和1.45cm,累积误差最大值分别为6.37cm和5.55cm。但由于采用了二维快速傅里叶技术和块对角最小二乘法,极大地提高了计算效率。本文数值结果说明Torus方法是一种独立有效的方法,可用于GOCE任务海量卫星引力梯度观测值反演重力场的快速解算。  相似文献   

CSD1800A型钻机在青藏高原应用效果及对比分析          下载免费PDF全文

徐国辉  梁俭  刘鹏  梅冬 《探矿工程》2015,42(10):42-44，54

介绍了CSD1800A型全液压动力头式钻机在青藏高原矿区内的应用效果,并对该型钻机在实际应用中的优点进行了分析;通过与国内外其它钻机的应用效果进行对比,认为CSD1800A型钻机是一款经济实用的中深孔钻机。  相似文献   

苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响     

孙文静  王晓艳  祁鹏志  张建设  吴常文 《海洋与湖沼》2018,49(4):897-903

为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。  相似文献   

改进A?的高层建筑逃生路径规划算法研究   总被引：1，自引：0，他引：1  

靳海亮  王赢乐  袁鸣  陈梦龙 《测绘通报》2019,(11):17-21,25

针对高层建筑内部结构复杂，发生火灾时没有疏散引导情况，逃生通道极易发生拥堵导致疏散效率降低的问题，本文基于对A*算法的改进，提出了高层建筑逃生路径规划算法。该算法以高层建筑内部路网节点为关键要素，综合火灾发生位置、人员密度、人员数量等因素，从逃生终点优化分配、节点扩展优化、权值优化3个方面进行改进，实现了火灾发生时高层建筑内部的逃生路径规划，并以某高层建筑为例，验证了本文算法的可行性。  相似文献   

Study of Dongting Lake area variation and its influence on water level using MODIS data / Etude de la variation de la surface du Lac Dongting et de son influence sur le niveau d’eau,grâce à des données MODIS     

《水文科学杂志》2013,58(1)

Abstract

Abstract MODerate-resolution Imaging Spectroradiometer (MODIS) is a new generation remote sensing (RS) sensor and its applications in hydrology and water resources have attracted much attention. To overcome the problems of slow response in flood disaster monitoring based on traditional RS techniques in China, the Flood Disaster Monitoring and Assessing System (FDMAS), based on MODIS and a Geographic Information System (GIS), was designed and applied to Dongting Lake, Hunan Province, China. The storage curve of Dongting Lake for 1995 was obtained using 1:10 000 topographic map data and then a relationship between water level at the Chenglingji hydrological station and lake area was derived. A new relationship between water level and lake area was obtained by processing MODIS images of Dongting Lake from April 2002 to April 2003 and the influence of lake area variation on water level was analysed with the 1996 flood data. It was found that the water level reduction reached 0.64 m for the 1996 flood if the original lake area curve was replaced with the area curve of 2002. This illustrates that the flood water level has been considerably reduced as a result of the increased area of Dongting Lake since the Chinese Central Government’s ?return land to lake? policy took effect in 1998.  相似文献