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胶州湾冬季异养细菌与营养盐分布特征及关系研究 总被引：7，自引：1，他引：7
2003年12月、2004年1月和2月下旬进行了3个航次的现场调查,对胶州湾冬季异养浮游细菌和无机营养盐分布特征、相互关系及影响因素进行了探讨。胶州湾冬季异养浮游细菌数量的月变化与水温的变化趋势基本一致,分布特征为近岸高,离岸逐渐减少,最大值出现在湾东北部,为7.55×109个/dm3,最小值位于湾中央,为1.89×109个/dm3。水体PO3-,NO-和NH+等营养盐的分布同样具有近岸较高,离岸逐渐减少的特点,异养细菌数量分布与氨盐浓度呈高度显著性正相关,相关系数为0.73(P<0.01),与硝酸盐和磷酸盐浓度呈显著性正相关关系,相关系数分别为0.50和0.48(P≤0.05)。水温是影响胶州湾细菌季节变化的主要因素之一,冬季营养盐水平分布可能与异养浮游细菌矿化有机质产生营养盐有关。 相似文献
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This study had the objective of quantifying the variability in abundance, cell volume, morphology and C content of a natural bacterioplankton community in a coastal zone of the North Adriatic Sea during two periods (February and June) of two consequent years (1996 and 1997). We used epifluorescence microscopy with Acridine Orange staining procedures and a microphotographic technique. Low variability in bacterial abundance (range 0.3–3.1 × 105 cells ml?1) occurred between summer and winter periods. Conversely, the cell volume and the calculated carbon content changed greatly with warm and cold periods (ranges: 0.015–0.303 μm3 and 5.83–42.17 fg C cell?1, respectively). Elongated bacteria were dominant while coccoid cells prevailed only in February 1997. Biomass showed high variability (range 0.12–10.21 μg C l?1) whilst the abundance did not show noticeable differences among the sampling periods. As a consequence, quantification of bacterial biomass based solely on cell abundance must be considered with caution because the true biomass could depend on variability in cell volumes and morphotypes. 相似文献
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通过提取山东半岛北岸不同类型潮间带海水样品的总DNA,构建16S r DNA文库,利用群落相似性分析(ANOSIM)和非度量多维尺度转换排序(NMDS),探究潮间带类型对浮游细菌群落结构的影响,并对比了与近海浮游细菌群落结构的差异。分析结果显示,浮游细菌的丰度及多样性受到潮间带类型的影响,烟台养马岛泥滩、石滩、辛安河沙滩、黄河三角洲碱蓬区和天鹅湖海草区以变形菌门占优势,而黄河三角洲米草区以拟杆菌门为优势菌,其中,天鹅湖海草区浮游细菌的丰富度和多样性最高。潮间带海水中浮游细菌的组成与近海存在显著差异,潮间带浮游细菌的丰度及多样性均显著高于近海。推测季节因素、植被类型、有机质来源可能是造成潮间带不同生境与近海浮游细菌多样性差异的重要因素。 相似文献
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模拟了厦门沿岸海区病毒裂解产物对于自然细菌群落的影响.实验过程中基于不同过滤方式收集的细菌、病毒种群和病毒裂解产物,通过室内模拟培养方式,结合流式细胞计数和总有机碳分析仪分析病毒、细菌丰度和有机碳含量等技术,研究浮游细菌在病毒裂解产物添加下的生长变化.实验结果显示,去除原生生物捕食者的浮游细菌丰度在整个培养过程中持续增加,特别是病毒裂解产物添加组中的细菌类群的增长程度明显,其丰度在5 d的培养时间内从2.76×105cells/cm3上升至1.90×106cells/cm3;而在无病毒裂解产物添加组中其丰度仅仅从3.0×105cells/cm3上升至1.39×106cells/cm3;相对于无病毒裂解产物添加组,培养周期内细菌丰度均值在病毒裂解产物添加组中的绝对增量约为3.64×105cells/cm3,这表明此研究海区的病毒裂解产物在模拟原位环境的培养体系中可快速地被环境中的其他细菌类群吸收、利用,并且在丰度上产生显著影响(p0.05).结合病毒丰度变化以及细菌细胞裂解释放量、细胞碳含量的关系估算了收集来的病毒裂解产物在培养体系中释放的碳量大约为2.12~7.67μg/(dm3·d);它被浮游细菌用于丰度增长方面的贡献大约22.47%~81.25%.此外,在培养过程中,溶解有机碳的浓度在病毒裂解产物添加组中的变化相对于无病毒裂解产物添加组缓慢,但总体上两者随时间变化逐渐减少,它们间的均值差值约为1.04μmol/dm3;结合对应的细菌丰度增量,估算出培养体系中浮游细菌碳含量大约为34.29fg/cell.上述结果揭示了厦门沿岸海域内病毒裂解产物在维持细菌群落多样性和生长繁殖方面的重要生态作用. 相似文献
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Distribution of microbial populations and their relationship with environmental variables in the North Yellow Sea, China 总被引：1，自引：0，他引：1
Xiaoge Bai Min Wang Yantao Liang Zhifeng Zhang Fang Wang Xuejiao Jiang 《中国海洋大学学报(英文版)》2012,11(1):75-85
In order to understand the large-scale spatial distribution characteristics of picoplankton,nanophytoplankton and virio-plankton and their relationship with environmental variables in coastal and offshore waters,flow cytometry(FCM) was used to ana-lyze microbial abundance of samples collected in summer from four depths at 36 stations in the North Yellow Sea(NYS).The data revealed spatial heterogeneity in microbial populations in the offshore and near-shore waters of the NYS during the summer.For the surface layer,picoeukaryotes were abundant in the near-shore waters,Synechococcus was abundant in the offshore areas,and bacte-rial and viral abundances were high in the near-shore waters around the Liaodong peninsula.In the near-shore waters,no significant vertical variation of picophytoplankton(0.2-2μm) abundance was found.However,the nanophytoplankton abundance was higher in the upper layers(from the surface to 10 m depth) than in the bottom layer.For the offshore waters,both pico-and nanophytoplankton(2-20μm) abundance decreased sharply with depth in the North Yellow Sea Cold Water Mass(NYSCWM).But,for the vertical dis-tribution of virus and bacteria abundance,no significant variation was observed in both near-shore and offshore waters.Autotrophic microbes were more sensitive to environmental change than heterotrophic microbes and viruses.Viruses showed a positive correla-tion with bacterial abundance,suggesting that the bacteriophage might be prominent for virioplankton(about 0.45μm) in summer in the NYS and that viral abundance might play an important role in microbial loop functions. 相似文献
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海州湾是南黄海赤潮高发区之一。为研究浮游细菌与赤潮及环境因子的关系,于2020年11月在海州湾赤潮期间进行了现场调查,采集了8个站位的浮游细菌、浮游植物样品,获取了温度、盐度、叶绿素a以及氮、磷、硅营养盐浓度等环境因子信息,分析了赤潮原因种及浮游细菌群落结构情况。结果显示,此次赤潮原因种为三叶原甲藻(Prorocentrum triestinum)、赤潮异弯藻(Heterosigma akashiwo)及塔胞藻(Pyramimonas sp.),发生赤潮时水温、盐度较低,盐度与赤潮异弯藻的分布有一定的联系。使用16SrRNA高通量测序方法分析调查海域的浮游细菌情况,结果显示赤潮发生站位的浮游细菌多样性略低于其他非赤潮发生站位,优势细菌与其他站位有明显不同,以假单胞菌目(Pseudomonadales)、红杆菌目(Rhodobacterales)和黄杆菌目(Flavobacteriales)为主。赤潮发生站位的浮游细菌群落结构也存在一定的差异,不同站位可能处于不同的赤潮阶段,对浮游细菌群落具有不同的影响。相关性分析显示,黄杆菌目与叶绿素a显著正相关,表明黄杆菌目与赤潮发生有一定的关系。 相似文献