Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>     

王振华  刘金成  苏爱  孙谧  王春波 《中国海洋湖沼学报》2009,27(4):835-844

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.  相似文献   

拮抗香蕉枯萎病菌海洋细菌的筛选和鉴定   总被引：3，自引：0，他引：3  

黄瑶  杨耿周  张雅娟  何秋韵  林巧玲  易润华 《广东海洋大学学报》2009,29(6):72-77

从93份中国南部海域近海海泥样品中分离出1800多株海洋细菌,以对峙培养法,测定海洋细菌对香蕉枯萎菌的拮抗作用,再以琼脂划线培养法(agar streak)测定菌株的抑菌活性,结果表明:约55.6%的菌株对香蕉枯萎菌有一定的拮抗作用,其中58株菌株抑制香蕉枯萎菌生长所形成的抑菌带宽度大于10.0mm,抑菌作用较好,复筛后其中13个菌株形成的抑菌带宽在15.0mm以上。经反复转接和对峙培养,筛选出拮抗效果好、抑菌活性稳定的菌株TC-1,初步鉴定为芽孢杆菌属的细菌,TC-1对14种病原微生物都有较好的抑菌效果。  相似文献   

Isolation and identification of bacteria associated with the surfaces of several algal species     

王子峰  肖天  逄少军  刘敏  岳海东 《中国海洋湖沼学报》2009,27(3):487-492

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on ...  相似文献   

近海沉积物与水体中天然大分子聚合物降解菌的原位富集与多样性分析          下载免费PDF全文

齐明明  李建洋  刘宪华  邵宗泽 《应用海洋学学报》2020,39(4):566-573

本研究选取5种海洋动植物大分子高聚物或其天然组织，包括虾壳、鱼鳞、海带叶片、几丁质和壳聚糖，分别在海水表层和沉积物环境中进行富集，定期取样，通过高通量测序分析菌群多样性。结果发现，不同有机物原位富集的细菌多样性存在较大的差异，而且同种底物在海水表层与沉积物中的降解菌菌群差异较大。从物种多样性看，在海水表层环境中富集的鱼鳞样品种群最丰富，而沉积物环境中富集的海带叶片样品菌群多样性最低，除其优势菌群为热袍菌门外，其他所有富集物中优势菌均为变形菌门。其中脱硫杆菌科、黄杆菌科、脱硫弧菌科和弧菌科占有较大比例，脱硫杆菌科在所有样品中优势较大，黄杆菌科在海水表层环境样品中为优势菌群，弧菌科在沉积物样品几丁质和壳聚糖样品中占比较高。本研究通过对同种海洋环境中不同富集样品之间以及同种底物不同环境中富集菌群之间的比较，分析结果得到了原位条件下参与大分子聚合物降解的菌群种类，但有待于在更多不同的海域富集物中进行验证。  相似文献   

Growth of marine bacteria and ammonium regeneration from substrates in different C: N ratios     

WANG Jiangtao  YIN Xiaonan 《海洋学报(英文版)》2009,28(3):59-64

Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C:N ratios (C:N_S) on the bacterial C:N ratio (C:N_B), the bacterial growth efficiency (BGE) and ammonium regeneration was mainly examined. The C:N_S ratios varied from 5:1 (carbon limitation) to 40:1 (nitrogen limitation) with varying combinations of glucose and NO₃^-. The C:N_B ratio had positive relationship with the C:N_S ratio (r=0.93, n=8), whose value was 3.77 when the C:N_S ratio was 5:1 but increased to 6.47 when the C:NS ratio was 40:1. These results indicate that the C:N_B ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C:N_s (r=-0.51, n=8). The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C:N_S ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source.  相似文献   

A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China     

YANG Bo  OUYANG Jianping  AO Jingqun  CHEN Xinhua 《海洋学报(英文版)》2009,28(1):62-71

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.  相似文献   

春秋季山东南部近岸浮游细菌生态分布   总被引：1，自引：0，他引：1  

肖慧  张喆  张艳  于庆云  唐学玺 《中国海洋大学学报(自然科学版)》2009,39(4)

分别于2007年4月(春季)和10月(秋季),对山东南部近岸海域进行了现场调查,研究了该海区浮游细菌丰度、生物量分布特征,探讨了它们与温度、溶解氧(DO)、总氮(N)、总磷(P)、硝酸盐(NO-3)、铵盐(NH+2)及活性磷酸盐(PO4-P)的相关性.结果表明:浮游细菌生物量具有一定的时间、空间分布特征,春季浮游细菌丰度及生物量要高于秋季,2个季节近岸细菌数量高于远岸区域;浮游细菌丰度及生物量与温度、DO、总P、NO-3、NH+2及PO4-P均呈显著相关关系(P<0.01),表明上述因子可能是该海域浮游细菌数量分布的主要限制因子.  相似文献   

宁波北仑港冬季浮游细菌多样性研究   总被引：3，自引：0，他引：3  

刘兵  李太武  苏秀榕  史西志  李登峰  杜莉利 《台湾海峡》2009,28(2):217-222

采用分子生物学方法对宁波北仑港冬季水体中的浮游细菌多样性进行了研究.提取水样中细菌基因组总DNA,以细菌16S rDNA通用引物进行PCR扩增,扩增产物经分子克隆、测序与序列分析,对水样中的细菌建立了16S rDNA克隆文库和系统发生树.结果表明,浮游细菌群落具有较高的多样性,34个克隆子分属2个不同的细菌类群,6个克隆子属于未知类群,优势细菌类群为Pro-teobacteria类群（变形菌类群）,占80%;细菌优势类群顺序为β-Proteobacteria类群（32.5%）、γ-Proteobacteria类群（25%）、α-Proteobacteria类群（15%）、ε-proteobacteria类群（7.5%）、Firmicutes类群（厚壁菌类群）（5%）.这一结果与近年来国内外有关港口微生物多样性的报道较为一致.用DNAStar中Clustalw程序从测序的40个克隆子中选出17个OTU进行系统发育分析,同样表明浮游细菌多样性较强.  相似文献   

Distribution of general aerobic heterotrophic bacteria in sediment core taken from the Canadian basin and the Chukchi Sea     

高爱国  陈皓文 《极地研究(英文版)》2008,19(1):14-22

The occurrence percentage and abundance of General Aerobic Hetero- trophic Bacteria （GAB） were determined by using the method of MPN for 182 sub- samples from 10 sediment cores taken from the Canadian basin and the Chukchi Sea at two different culturing temperatures. The results showed that the general occurrence percentage of GAB was quite high, average abundanees of GAB at cultured temperatares of 4℃ and 25℃ were 4.46 ×10^7 and 5.47×10^7 cells·g^-1（wt）, respectively. The highest abundance of GAB occurred at 20 -22 cm section in the sediment. GAB abundances changed among the section of sediments, but there is a trend ： the a-bundances at the middle or lower sections were lower than those at upper section. Cultivation at 25℃ could improve the occurrence percentage and abundances of GAB, which suggests that the increasing of temperature may change the living circum-stances of GAB. The differences of GAB among the latitudes areas indicated that occurrence percentage and abundances of GAB in middle latitude areas were higher than those in the higher or lower latitude areas, and were more obvious at 4℃ than those at 25℃. The GAB abundances in sediment under the shallower water seemed to be low- er than those in sediments under the deeper water and this status was more obvious at 25℃ than that at 4℃.  相似文献   

页岩油评价的关键参数及求取方法研究   总被引：1，自引：0，他引：1  

王敏 《沉积学报》2014,(1)

随着非常规油气勘探的进一步深入,页岩油气逐渐成为现今勘探研究的热点和难点。目前国内针对页岩油的勘探开发没有成功的案例可循,实际工作中一直沿用了国外页岩气的勘探及评价思路。通过研究指出,页岩油自身的特点决定了其勘探不能照搬国外的页岩气评价思路,将研究重点仅仅停留在有机碳含量上无法满足勘探需要,页岩油勘探的核心问题在于地层已生成的游离烃含量的评价。利用胜利油区第一口页岩密闭取芯井分析化验资料为基础,系统研究了孔隙度与单位含油体积以及含水体积之间的关系,发现了烃源岩中孔隙性越好,含油性越好的特点。利用孔隙度、含油饱和度等岩心分析数据推导出了反映泥质页岩游离烃含量的评价模型,并构建了基于实测的深侧向电阻率、孔隙度、有机质成熟度等参数的求取模型。利用页岩地层游离烃中有机碳含量和总有机碳含量对比可以直观反映地层中游离烃含量的垂向变化规律,指示页岩层段的有利油气区段。以胜利油田沾化凹陷罗家地区罗69井实验分析资料为例,对模型的参数求取及效果分析进行了阐述,构建的地层有机质成熟度模型及游离烃中有机碳含量均精度较高,在此基础上,通过游离烃有机碳含量绝对值、以及游离烃中有机碳含量与地层有机碳含量的比值实现了对页岩油地层可动油气富集带的指示,可动油气相对富集的层段即为页岩油地层的勘探目标。该思路可为目前的陆相页岩油勘探提供借鉴和参考。  相似文献