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Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA(RAPD) 总被引:1,自引:1,他引:0
Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well. 相似文献
64.
Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa
沈颂东 《中国海洋湖沼学报》2008,26(4)
In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis. 相似文献
65.
XU Kefeng LI Qi College of Fisheries Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2009,8(2)
The inheritance mode of seven microsatellite markers was investigated in Patinopecten yessoensis larvae from four con-trolled crosses,and the feasibility of using these markers for kinship estimation was also examined. All the seven microsatellite loci were compatible with Mendelian inheritance. Neither sex-linked barriers to transmission nor major barriers to fertilization between gametes from the parents were evident. Two of the seven loci showed the presence of null alleles in two families,suggesting the... 相似文献
66.
本研究利用核糖体大亚基5'端序列PCR-RFLP以及转录单元内间隔区(Internal TranlsclqIbed Sppacer,rrS)序列分析相结合的方法,首次对福建东山岛附近海域3种优势种类造礁石珊瑚共生藻进行了分子系统分类和遗传多样性研究.PCR-RFLP分析发现东山岛附近海域3种优势种类造礁石珊瑚共生藻都属于C系群共生藻,而ITS序列的序列分析结果表明东山岛附近海域3种优势种类造礁石珊瑚共生藻都属于C1亚系群.研究结果表明ITS序列进化速度快,适合于造礁石珊瑚共生藻属亚系群水平的鉴定.而东山岛附近海域造礁石珊瑚共生藻的多样性低,暗示东山岛附近海域造礁石珊瑚共生藻共生系统面对外界环境压力的适应能力较低. 相似文献
67.
综述了虫黄藻的分类研究,其中与造礁石珊瑚共生的虫黄藻主要是共生藻属(Symbiodinium)的种类,重点概述了共生藻的分类和遗传多样性研究进展,特别综述了分子生物学技术在造礁石珊瑚共生藻的分类和遗传多样性方面的研究近况,并对未来共生藻的分类和遗传多样性研究作了展望.目前多采用分子生物学手段进行共生藻的分类和遗传多样性研究,PCR-RFLP是解决共生藻系群水平分类的有效分子标记,而DNA序列分析是目前进行共生藻分子进化和系统发育研究最有效的方法.目前应用于共生藻分子系统发生研究的DNA信息主要为核糖体RNA.对共生藻进行分类和遗传多样性研究,将有助于理解造礁石珊瑚共生藻共生系统对外界环境变化的生态响应机制. 相似文献
68.
利用荧光标记扩增片段长度多态性(fAFLP)技术对文蛤(Meretrix meretrix)、青蛤(Cyclina sinensis)、菲律宾蛤仔(Ruditapes philippinarum)和硬壳蛤(Mercenaria mercenaria)4种帘蛤科贝类的群体遗传多样性和种间关系进行了研究。选择EcoRⅠ/MseⅠ进行酶切,使用6个E 3/M 3引物组合进行扩增,共获得1 096个位点,多态位点比率95.1%,片段长度50~456 bp。其中,文蛤、青蛤、菲律宾蛤仔和硬壳蛤分别得到681,715,702和694个位点,相应的多态位点比率为76.8%,81.7%,83.0%和75.1%,得到17个种特异性位点,可作为4物种特征标记。分析了群体遗传相似系数和遗传多样性指数以及种间遗传相似系数。结果表明,硬壳蛤群体遗传相似系数最高(0.670 9),遗传多样性指数最低(0.236 0);菲律宾蛤仔群体遗传相似系数最低(0.592 5),遗传多样性指数最高(0.261 8);根据遗传相似系数采用UPGMA法构建了4物种32个体的聚类图,表明文蛤与菲律宾蛤仔遗传关系最近,青蛤与其他3物种遗传关系较远。 相似文献
69.
Genetic diversity of two wild Penaeus monodon populations sampled from the coastal waters of Qinglan ( Hainan Province of China, HN) and Malaysia (KD), and the F1 generation of a Thailand broodstock population (CP) were examined by vertical polyacrylamide gel electrophoresis. Of 21 loci encoded by ten enzymes, 11 were polymorphic. The mean proportions of polymorphic loci of HN, KD and CP were 36. 36%, 45.45% and 50.00% , with the average heterozygosities of 0.135, 0.181 and 0.191, and the effective numbers of alleles per loci were 1. 300, 1. 330 and 1. 329, respectively. The divergent indexes of HN, KD and CP were0.023, 0.124, and 0.117, respectively. The genetic distance between the two wild populations was 0.005, and the gene differentiation coefficient was also very low (0.014). The results indicated that the F1 generation population had a higher genetic diversity than the two wild populations, and that the HN population had the lowest one. There was no significant differentiation between HN and KD populations and all populations existed with a slight heterozygote excess. 相似文献
70.