苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响     

孙文静  王晓艳  祁鹏志  张建设  吴常文 《海洋与湖沼》2018,49(4):897-903

为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。  相似文献   

A Long Way to Go: Collective Paths to Racial Justice in Geography     

Caroline Faria  Bisola Falola  Jane Henderson  Rebecca Maria Torres 《The Professional geographer》2019,71(2):364-376

Despite decades of recognition and worry about diversity, our discipline remains persistently white. That is, it is dominated by white bodies and it continues to conform to norms, practices, and ideologies of whiteness. This is a loss. At best, it limits the possibilities and impact of our work as geographers. At worst, it perpetuates harmful exclusions in our discipline: its working environments, its institutions, and its knowledge production. This remains deeply concerning for many geographers, and there has been important research, commentary, and institutional activity over the years. Yet, research shows us that little meaningful progress has been made. We know that mentoring is one vital part of the journey toward change. As such, we reflect here on our experience developing a research collective built on a transformative mentoring practice. We outline the key challenges, strategies, and tentative successes of the collective in supporting women of color undergraduate, graduate, and faculty geographers, arguing that such feminist formations are a vital part of the path to intellectual racial justice in our field. Key Words: diversity, feminist geography, higher education, mentoring, race.  相似文献   

苯并（a）芘诱导下岩虫CYP基因的表达特征     

李婉娟  赵欢  Ba Fuyang  李绍娟  孙秀鹏  杨大佐  周一兵 《海洋学报(英文版)》2019,38(6):46-53

Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.  相似文献   

Improved spatial delineation of streambed properties and water fluxes using distributed temperature sensing          下载免费PDF全文

Landon J. S. Halloran  Hamid Roshan  Gabriel C. Rau  Martin S. Andersen  R. Ian Acworth 《水文研究》2016,30(15):2686-2702

A new method was developed for analysing and delineating streambed water fluxes, flow conditions and hydraulic properties using coiled fibre‐optic distributed temperature sensing or closely spaced discrete temperature sensors. This method allows for a thorough treatment of the spatial information embedded in temperature data by creating a matrix visualization of all possible sensor pairs. Application of the method to a 5‐day field dataset reveals the complexity of shallow streambed thermal regimes. To understand how velocity estimates are affected by violations of assumptions of one‐dimensional, saturated, homogeneous flow and to aid in the interpretation of field observations, the method was also applied to temperature data generated by numerical models of common field conditions: horizontal layering, presence of lateral flow and variable streambed saturation. The results show that each condition creates a distinct signature visible in the triangular matrices. The matrices are used to perform a comparison of the behaviour of one‐dimensional analytical heat‐tracing models. The results show that the amplitude ratio‐based method of velocity calculation leads to the most reliable estimates. The minimum sensor spacing required to obtain reliable velocity estimates with discrete sensors is also investigated using field data. The developed method will aid future heat‐tracing studies by providing a technique for visualizing and comparing results from fibre‐optic distributed temperature sensing installations and testing the robustness of analytical heat‐tracing models. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

基于“一张图”平台的国土资源执法监察系统设计与研究     

董星星 《测绘与空间地理信息》2016,(1):153-155

从土地执法监察管理的实际需求出发,探讨了基于"一张图"平台的国土资源执法监察系统建设方案,设计了土地执法监察系统结构和功能模块,并进一步给出了土地执法监察数据库建设和数据交换的技术方法。该系统方案的应用将有利于快速、准确地监控国土资源违法行为,强化对国土资源违法行为的监控能力,有效地提高了国土部门执法监察工作效率和执法水平。  相似文献   

TRMM卫星降水数据在小尺度流域的评估与应用          下载免费PDF全文

周萌  江善虎  任立良 《水文》2016,36(1):22-26

选取史灌河流域上游黄泥庄站以上集水区域为研究流域,以地面站点观测为基准数据,评估TRMM 3B42V7版本卫星降水数据的精度,并采用上述两种降水数据驱动栅格型新安江模型,模拟黄泥庄站日径流和月径流过程。结果表明:2001~2010年TRMM 3B42V7降水数据与雨量站数据累积量的偏差不大,仅为1.71%,但相关系数较低,采用TRMM降水数据模拟的日径流能基本再现黄泥庄站的日径流过程,但对洪峰的模拟精度较低;TRMM月降水数据精度较高,相关系数为0.96,能够较为精确地模拟黄泥庄站的月径流过程。  相似文献   

流速仪信号采集系统设计   总被引：1，自引：1，他引：0       下载免费PDF全文

金福一 《水文》2016,36(1):88-91

介绍了流速仪检定系统的工作原理,分析流速仪信号特点和采集难点,针对流速仪信号受外界干扰信号波形复杂问题,设计信号采集硬件调理电路。针对流速仪信号周期多样性问题,对测频法和测周法进行误差分析和比较,确定在不同速度区间使用不同软件滤波的方法。通过硬件调理电路及软件滤波仿真验证,证明设计的可行性,可有效提高流速仪信号采集的精度,并在单片机平台上完成了硬件设计。  相似文献   

长江口及其邻近海域叶绿素a分布特征及其与低氧区形成的关系   总被引：2，自引：0，他引：2  

李照  宋书群  李才文 《海洋科学》2016,40(2):1-10

于2013年3月和8月研究了长江口及其邻近海域叶绿素a的分布特征,并对环境因子和长江冲淡水对浮游植物生物量分布的影响进行了探讨。结果表明,叶绿素a浓度在丰水期较高,平均值为5.18μg/L,最高值达32.05μg/L,现场海水出现变色现象;与同期历史资料对比分析,发现该海域叶绿素a浓度呈现出波动增长趋势。丰水期与枯水期叶绿素a的相对高值区均位于冲淡水的中部,122.5°E~123°E之间;丰水期在调查海域出现溶解氧低值区与低氧区,最低值仅为0.64 mg/L;发现低氧区出现位置北移、面积扩大和溶解氧最低值下降的趋势。底层溶解氧低值区分布与表层叶绿素高值区大致吻合,表明低氧现象与表层浮游植物的生长和现存量密切相关,在跃层存在的水体中表层浮游植物的大量繁殖易造成底层低氧区的出现。  相似文献   

Hispanic Immigrants' Vulnerabilities to Flood and Hurricane Hazards in Two United States Metropolitan Areas          下载免费PDF全文

Alejandra Maldonado  Timothy W. Collins  Sara E. Grineski 《Geographical review》2016,106(1):109-135

Research reveals that disasters are disproportionately debilitating for marginalized social groups. Numerous studies have examined racial/ethnic dimensions of disaster vulnerability, but few have focused on Hispanic immigrants. More research on Hispanic immigrants is needed, since they constitute a major component of the Hispanic population—the largest and fastest‐growing minority group in the U.S.—and because they experience distinctive cultural and immigration status disadvantages. We examine the flood/hurricane vulnerabilities of Hispanic immigrants in comparison to U.S.–born Hispanics and non‐Hispanic whites. Using mixed methods to analyze data from 429 surveys and 31 interviews with residents living in flood zones, we examine differences in self‐protective action, risk perception, and hazard knowledge between the three groups in Houston and Miami. Hispanic immigrants exhibited lower levels of self‐protection and hazard knowledge, and higher perceptions of risk, which reflects their heightened vulnerability. Risk‐reduction programs should target the particular vulnerabilities of Hispanic immigrants, and future studies should examine their vulnerabilities in other contexts.  相似文献   

Potential Influence of a Developing La Niña on the Sea-Ice Reduction in the Barents–Kara Seas     

Rui Luo  Peng Zhang  Juan Dou 《大气与海洋》2013,51(3):182-194

Abstract

The potential influence of a developing La Niña on Arctic sea-ice annual variability is investigated using both observational data and an atmospheric general circulation model. It is found that during the developing phase of an eastern Pacific (EP) La Niña event in June, July, and August (JJA) and September, October, and November (SON), the sea-ice concentration (SIC) over the Barents–Kara Seas declines more than 15%. The local atmospheric circulation pattern associated with the EP La Niña is characterized as a weak decrease in geopotential height over the Barents–Kara Seas, combined with an anticyclone in the North Atlantic. The corresponding southerly winds push warm waters northward into the key sea-ice reduction region and directly accelerate sea-ice melt. Meanwhile, the abundant moisture contained in the lower troposphere is transported into the Arctic region by winds resulting from the local barotropic structure. The humid atmosphere contributes to both net shortwave and longwave radiation and thus indirectly accelerates the decline in sea ice. Simulations by the European Centre Hamburg Model, version 5.4, are forced by observed sea surface temperature anomalies associated with EP La Niña events. The results of the simulations capture the North Atlantic anticyclone and reproduce the moisture transport, which supports the premise that an EP La Niña plays a crucial role in sea-ice reduction over the Barents–Kara sector from the perspective of atmospheric circulation and net surface heat flux.  相似文献