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Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. 相似文献
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Polychaetes provide an excellent food resource for fish and represent the dominant zoobenthos in marine ecosystems. Diel variation in the rates of metabolism and ammonia-N excretion of Marphysa sanguinea were studied. The worms were grouped according to their wet body weight into small (S; 1.24±0.06 g), medium (M; 4.00±0.30 g), and large (L; 8.54±1.08 g) categories. Their weight-specific metabolic rates, based on aerobic respiration (R), were measured at 16°C (±0.2°C) and classed as either routine (R R) or standard (R S) rates. Both respiration types decreased with increasing body weight. Respiration was described by R = a W b, where b was -0.400 9 and -0.532 0 for R R and R S, respectively. Diurnal changes in R S for each group was relatively flat, with a slightly increasing trend with time, but was relatively stable as a whole. R R of the diurnal variation of worms was higher than R S, but both had similar overall trends. The peak values of specific dynamic action (SDA) (R SDA) in the S, M, and L groups were 2.704, 1.149, and 0.682 mg/(g?h), respectively. The durations of SDA were 13, 6, and 6 h, respectively and the energy expenditures of SDA were 377.98, 117.34, and 74.94 J/g, respectively. These data indicate that the metabolic rates were higher in smaller individuals, which is advantageous for their rapid growth. 相似文献
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The influence of cadmium on the antioxidant enzyme activities in polychaete Perinereis aibuhitensis Grube (Annelida: Polychaeta) 总被引：1，自引：0，他引：1
The infaunal polychaete Perinereis aibuhitensis Grube, distributed widely along Asian coasts and estuaries, is considered a useful animal model in ecotoxicological tests
and a promising candidate in biomonitoring programs. This paper deals with the activities of antioxidant enzymes including
superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidases (GSH-Px) in infaunal polychaete P. aibuhitensis exposed to a series of sublethal water-bound cadmium (Cd) concentrations (0, 0.34, 1.72, 3.44, 6.89, and 17.22 mg L−1) under a short-term exposure (1–8 d). The results indicate that the SOD and GSH-Px activities in P. aibuhitensis are stimulated first and then renewed to the original level. The CAT activity of worms decreases at an earlier exposure time
but increases to the control values at a later exposure time. Our study suggests that Cd can interfere with the antioxidant
defense system of P. aibuhitensis. However, the changes in antioxidant enzyme activities for this species do not show the best promise as biomarkers in Cd
biomonitoring of estuarine and coastal zones because weak or non-dose-effect relationships between the antioxidant enzymes
activities and Cd levels are found. 相似文献
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通过周年取样、种类鉴定和生物量统计分析,对黄海北部大连地区仿刺参养殖池塘浮游植物种类组成的季节演替和生物量周年变化进行了研究。结果表明:该地区仿刺参养殖池塘中浮游藻类组成依次为硅藻门、蓝藻门、绿藻门和甲藻门,硅藻门和绿藻门作为池塘主要优势种全年均有分布;实验池塘浮游藻类年平均生物量为20.91 mg/L,各池塘浮游植物生物量季节变化趋势相同,最大生物量出现于8月初,为46.18 mg/L,最小生物量出现在2月,为2.26 mg/L。分析表明,浮游植物生物量与水温、透明度成极显著的相关性,并受硝酸氮、磷酸盐、硅酸盐及硝酸氮与磷酸盐的交互作用影响。 相似文献
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