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四种蟹成蟹消化酶的研究 总被引:10,自引:0,他引:10
比较中华绒螯蟹、锯缘青蟹、三疣梭子蟹和长江华溪蟹胃、肠、肝胰腺的五种消化酶(胃蛋白酶、类胰蛋白酶、脂肪酶、淀粉酶、纤维素酶)活力,结果表明四种蟹的类胰蛋白酶比活力是胃蛋白酶的7~17倍,淀粉酶是纤维素酶的30~183倍,脂肪酶活力较低。消化酶活力大小具有器官特异性和种间差异。 相似文献
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This study expands on recent reports that direct development in the Chinese potamid freshwater crab Sinopotamon yangtsekiense involves the completion of all brachyuran larval stages (nauplius, zoea, and megalopa) inside the egg case during embryonic development. Detailed studies of embryonic development in this species revealed the presence of an additional larval stage (the egg-juvenile) between the megalopa and the free-living hatchling crab. We described and compared the appendages of the head, thorax, and abdomen of the egg-juvenile with those of the hatchling crab in S. yangtsekiense. Significant differences were found between most of the appendages of these two stages with a soft exoskeleton in the egg-juvenile, no joint articulation, a slimmer appearance, and a lack of setae when compared with the newly emerged free-living hatchling crab. These modifications of the appendages are related to the confinement within the egg case of the egg-megalopa and egg-juvenile during direct development, and the need for the free-living hatchling freshwater crab to move, feed, and respire. In marine crabs, the megalopa gives rise to the first crab stage whereas in freshwater crabs the egg-juvenile follows the megalopa and immediately precedes the free-living first crab stage. 相似文献
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镉诱导长江华溪蟹(Sinopotamon yangtsekiense)心肌细胞凋亡的研究 总被引:1,自引:0,他引:1
采用急性毒性实验方法,研究了不同浓度氯化镉(0、7.25、14.50、29.00、58.00和116.00mg/L)处理不同时间(1d、3d、5d和7d)对长江华溪蟹心肌细胞凋亡的影响。结果表明,低浓度组及染毒时间较短时镉对心肌的影响不明显,但随着染毒浓度的增加和时间的延长,心肌出现空泡变性、玻璃样变性、炎细胞浸润等病理现象,在染毒7d虽然观察到疑似的凋亡细胞,但凋亡率较低;荧光显微镜未观察到心肌细胞典型的凋亡细胞核形态学变化;琼脂糖凝胶电泳图谱未出现凋亡细胞所特有的梯状条带;流式细胞术检测结果显示镉处理组细胞凋亡率增加,但与对照组相比,无统计学意义(P>0.05);Caspase-3活性也没有显著性变化(P>0.05)。因此,在本实验所选取的浓度和时间范围内,镉未诱导长江华溪蟹心肌细胞发生细胞凋亡,这与本课题组前期研究中观察到的肝胰腺、精巢组织在染毒2d、7d发生细胞凋亡的情况明显不同,这可能与心脏组织的特殊性、不同组织对镉的积累量不同有关。 相似文献
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大型底栖动物群落在河流生态系统中发挥着重要作用,2003年6月至2004年6月间对汉江流域2级河流——黑竹冲河大型底栖动物优势种类的生活史和生产力进行为期一周年的研究.结果表明:主要优势种圆顶华溪蟹(Sinopotamon teritisum Dai et al.)生活史周期基本为四年,每年8—10月为主要繁殖季节,衰老个体(体宽〉29mm)在每年的1—2月份死亡较多;现存量在一周年中变化较大,密度9月份达到峰值,为35ind/m^2,生物量在6月达到峰值,为41.7g/m^2;采用体长频率分布法(size—frequency method)测算的周年生产量为151,3071g/(m^2.a),P/B系数为1.4.在其胃内含物中,无形态碎屑占45.4%,虾占10.9%、水生昆虫占35.6%、螺占5.7%、植物组织占8.1%,对次级生产量的贡献率分别10.84%、18.21%、59.49%、9.52%和1.94%. 相似文献
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