复垦土地样品中石油类物质加速溶剂萃取-荧光分光光度法分析方法研究     

赵江华  王鹏  黎卫亮  李忠煜 《岩矿测试》2021,(3):375-383

作为土地环境质量的一项重要指标,石油类物质含量决定了土地的用途。对于复垦土地样品,在一定程度上反映了污染土地的复垦、治理效果。土壤样中的石油类物质测定方法主要有气相色谱法、红外分光光度法、紫外光谱法等。气相色谱法主要应用于测定样品中的饱和烷烃;红外分光光度法通过测定不同波数下的特征吸收值来表征样品中石油类物质,但采用四氯乙烯为溶剂,试剂环境危害大,前处理效率低,检出限高。本文针对复垦土地样品中石油类物质含量不高,组分多为难挥发且结构复杂、基质干扰大的特点,采用正己烷为溶剂、加速溶剂萃取(ASE)前处理,荧光分光光度计测定样品中的石油类物质。以海洋环境监测石油标准物质为标准样品配制工作曲线,线性相关系数r~2=0.9997;方法检出限为0.40mg/kg,检测下限为1.60mg/kg;实际样品方法精密度为1.10%～8.76%;基质加标回收率为89.0%～95.7%;检测实际样品的结果与现行有效的HJ 1051—2019红外分光光度法基本一致,高含量样品的测定结果高于红外法。本方法所用溶剂正己烷的毒性小于四氯乙烯,前处理方法ASE的自动化程度高,萃取效率高,精密度提高11.5%～67.3%。对于石油类组分结构相对复杂、难提取的样品,本方法检出限低于红外法(4mg/kg)。  相似文献   

吡啶偶氮类试剂在光度分析中的应用进展   总被引：1，自引：1，他引：0  

于秀兰  田松涛  孟凡金 《岩矿测试》2011,30(2):131-137

吡啶偶氮类试剂是一类性能优良的络合滴定指示剂和光度显色剂,迄今已合成了200余种,在分光光度分析测定各种金属离子方面得到了广泛的应用。为了进一步改善这类试剂的灵敏度和选择性等分析性能,近年深入研究了吡啶偶氮类试剂的结构与性能的关系,进而相继开发出一系列性能优异的吡啶偶氮类试剂。文章综述了1990—2010年以来常见的吡啶偶氮类试剂及其在分光光度分析测定金属离子方面的进展。从反应体系、最大吸收波长、灵敏度、线性范围等方面归纳了吡啶偶氮类试剂在实际样品中的应用情况,为此类试剂的开发和应用提供一些理论依据。  相似文献   

Surfactant Enhance DLLME/FO‐LADS: Assay of Malachite Green Level in Aquatic Environment of Trout Fish     

Araz Bidari  Mohammad Reza Ganjali  Parviz Norouzi 《洁净——土壤、空气、水》2011,39(1):83-87

Malachite green (MG), a traditional agent used in aquaculture although is not approved; its low cost and high efficacy make illicit use likely. We developed a small‐scale, simple, and sensitive dispersive liquid–liquid microextraction procedure for the assay of trace amounts of MG in aquatic environment of Trout fish. Fiber optic‐linear array detection spectrophotometry with charge‐coupled device detector benefiting from a microcell was used for this purpose. The method is based on enhancement effect of an anionic surfactant on the extraction of MG in to very fine multidroplets of microextraction solvent which made assisted by disperser solvent. Under the optimum conditions, the enrichment factor 77.5 was obtained from a 5‐mL water sample. The calibration graph was linear up to 5 × 10^?7 mol L^?1 with detection limit of 1 × 10^?8 mol L^?1. The relative standard deviation for seven replicate measurements of 4 × 10^?7 and 5 × 10^?8 mol L^?1 of MG were 3.3 and 4.5%, respectively.  相似文献   

原子吸收分光光度法快速测定铬铁矿中钙、镁、锰   总被引：2，自引：0，他引：2  

熊艳 《安徽地质》2006,16(4):270-272

通过在标液中加入相应的Sr来消除离子干扰,采用原子吸收分光光度法快速测定CaO、MgO、MnO的含量,方法经国家标准物质验证,其RSD<2%(n=6)。其结果与标准值相符。方法达到了准确、简便、快速、实用性强。  相似文献   

分光光度法测定海洋河弧菌510脂多糖     

张运涛  顾谦群  方玉春  吕元萌  管华诗 《中国海洋大学学报(自然科学版)》2003,33(3):355-360

本文研究了直接测定海洋河弧菌 (vibrio fluvailis) 5 10脂多糖的分光光度法。利用 1,9-二甲基亚甲蓝 (DMB) ,在适当条件下同脂多糖发生染色反应 ,在 5 5 0 nm波长下 ,反应体系的吸光值同脂多糖含量呈线性相关。相关系数为 0 .9993,最低检出限为 10 μg/m L,相对标准偏差为 1.6 2 ,平均回收率为 (10 5± 2 .18) %。  相似文献   

Physical parameters of the continuum emission layer of Mira type stars. II. Radii and expansion velocities     

E. V. Ruban  A. A. Arkharov  E. I. Hagen-Thorn  T. Yu. Puzakova 《Astrophysics》2007,50(4):460-466

The Pulkovo Spectrophotometric Data Base is used to determine the expansion velocities and radii of the continuum emission layers of the stars RR SCO and OMICR CET. Dependences of the radii and velocities on the phase of the variability cycle are found which agree with the known values obtained from the layers which emit line radiation. __________ Translated from Astrofizika, Vol. 50, No. 4, pp. 557–564 (November 2007).  相似文献   

新显色剂四-(3-磺基-4-氯苯基)卟啉分光光度法测定微量铜          下载免费PDF全文

张文娟  赵伟  周长祥 《山东国土资源》2014,30(9)

研究了新显色剂四-(3-磺基-4-氯苯基)卟啉在微酸性介质中,表面活性剂OP与CPC存在下测定微量铜的显色反应。络合物在波长420 nm处有最大吸收峰,摩尔吸光度系数为3.44×105L·mol-1cm-1,用双峰双波长光度法测定摩尔吸光系数为6.72×105L·mol-1·cm-1,铜含量在0.5~4.5μg/25 m L范围内符合比耳定律。方法应用于矿样中微量铜的测定,结果令人满意。  相似文献   

分光光度法测定水系沉积物中硼     

于微 《吉林地质》2014,(4)

本文介绍了分光光度法测定硼的分析方法,盐酸介质中,硼与甲亚胺-H生成可溶性黄色络合物,用饱和Ba CO3溶液沉淀去除其干扰,于420.0 nm进行比色。方法的检出限为29μg/g,结果满足国家标准物质和实际样品分析测试要求,达到了国家标准。  相似文献   

PSF-fitting techniques for crowded field 3D spectroscopy     

Martin M. Roth 《New Astronomy Reviews》2006,49(10-12):573

From a historical point of view, it was only through the advent of the CCD as a linear, high dynamic range panoramic detector that it became possible to overcome the source confusion problem for stellar photometry, e.g., in star clusters or nearby galaxies. The ability of accurately sampling the point-spread-function (PSF) in two dimensions and to use it as a template for fitting severely overlapping stellar images is of fundamental importance for crowded-field photometry, and has thus become the foundation for the determination of accurate color-magnitude diagrams of globular clusters and the study of resolved stellar populations in nearby galaxies. Analogous to CCDs, the introduction of integral field spectrographs has opened a new avenue for crowded-field 3D spectroscopy, which benefits in the same way from PSF-fitting techniques as CCD photometry does. This paper presents first experience with sampling the PSF in 3D spectroscopy, reviews the effects of atmospheric refraction, discusses background subtraction problems, and presents several science applications as obtained from observations with the PMAS instrument at Calar Alto Observatory.  相似文献   

荧光分光光度法测定海洋生物中的谷胱甘肽     

张静  吉宏武  周静  陈成成  章超桦 《广东海洋大学学报》2005,25(4):32-34

对应用荧光分光光度法测定海洋生物中还原型谷胱甘肽(GSH)含量的方法进行了研究,并测定了分属于鱼、虾、贝、藻的10种海洋生物中谷胱甘肽的含量。利用邻苯二甲醛与GSH反应构成的荧光体系,在激发波长为365nm,发射波长为425nm的条件下,方法的回收率为99.22%~99.69%,变异系数为2.16%。应用此方法测得10种海洋生物中谷胱甘肽的含量为:红笛鲷(Lutjanussanguineus)0.399mg/g,银鲳(Pampusargenteus)0.352mg/g,大海鲢(Megalopscyrinoides)0.561mg/g,尖紫蛤(Sanguinolariaacuta)0.289mg/g,菲律宾蛤仔(Ruditapesphilippinarun)0.287mg/g,墨吉对虾(Penaeusmerguiensis)0.892mg/g,凡纳滨对虾(Litopenaeusvannamei)1.434mg/g,囊藻(Colpomeniasinu-ose)0.221mg/g,石莼(Ulvalactucal)0.727mg/g,马尾藻(Sargassummuticum)0.137mg/g。  相似文献