鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响   总被引：2，自引：5，他引：2  

陈师勇  张培军  莫照兰  邹玉霞  张振冬  徐永立 《海洋与湖沼》2004,35(2):159-166

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。  相似文献   

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

关于“包装产品的计量标准型一次抽检方案”的确定     

陈峥  赵维谦  李晓川 《中国海洋大学学报(自然科学版)》1989,(4)

本文给出了“包装产品的计量标准型一次抽检方案”,并通过一个实例说明本方案比一般《计量标准型一次抽检方案》更为优越。  相似文献   

北京市基础测绘技术规程的探讨   总被引：1，自引：1，他引：0  

刘红霞  晁春浩 《北京测绘》2008,(1):29-31

分析了规程与现行相关法律、法规和标准的关系,探讨了北京市基础测绘技术规程的内容,介绍了其创新点和应用情况。通过分析介绍,该地方标准统一了北京市基础测绘的技术标准,规范了基础测绘成果,有利于实现测绘信息资源的共享和有效利用。  相似文献   

磷处理粉煤灰可作农业土壤磷源   总被引：2，自引：0，他引：2  

申俊峰  M.A.Powell  D.B.Hayden 《地学前缘》2008,15(6):112

粉煤灰(简称CCP或ash)具有改良土壤,增加植物产量的功能。此外,由于它特殊的多孔结构,也可用作吸纳并承载植物养分的载体。用NaH2PO4配制成含磷0.10mol/L浓度的溶液,对采自加拿大西安大略省Sarnia地区Lambton电厂的底灰(bottomash)进行振荡浸渍处理。结果表明,振荡浸渍66h后的粉煤灰中磷含量可达784×10-6。以磷处理粉煤灰、未进行磷处理的粉煤灰和石英砂按比例混合,作为基本生长介质进行玉米种植实验,其中实验配方设计为生长介质中含磷量分别为标准含磷浓度(50×10-6)的10%、25%、50%、75%和100%。生长26、34和46d后分别与不含磷的空白配方、施加含氮-磷-钾为0-20-0标准磷肥并控制磷含量为标准浓度(50×10-6)配方进行生物产量对比。生长实验结果表明,以磷处理粉煤灰供磷的生长介质,当含磷量为标准浓度的25%至100%时,其植物生长量就比添加标准浓度磷肥的配方好。种植46d后的生物生长量统计结果显示,含磷分别为标准浓度50%、75%和100%的实验介质中,玉米杆的鲜重较施标准磷肥介质中玉米分别增长39.46%、42.73%和46.13%;玉米杆干重依次增加29.71%、13.39%和28.87%;根鲜重平均增加16.62%;根干重平均增加14.03%。上述实验结果启示,粉煤灰可以很好地吸纳承载磷养分,并持续供给植物吸收生长,如果采用吸纳磷(或其他养分)的粉煤灰改良砂质土壤将有重要意义。  相似文献   

Carbonyl products of the gas phase reaction of ozone with 1,1-disubstituted alkenes     

Eric Grosjean  Daniel Grosjean 《Journal of Atmospheric Chemistry》1996,24(2):141-156

Carbonyl products have been identified and their formation yields measured in experiments involving the gas phase reaction of ozone with 1,1-disubstituted alkenes at ambient T and p=1 atm. of air. Sufficient cyclohexane was added to scavenge the hydroxyl radical in order to minimize OH-alkene and OH-carbonyl reactions. Formation yields (carbonyl formed/ozone reacted) of primary carbonyls were close to the value of 1.0 that is consistent with the mechanism: O₃+R₁R₂C=CH₂ (HCHO+R₁R₂COO)+(1–)(R₁COR₂+H₂COO) where formaldehyde and the ketone R₁ COR₂ are the primary carbonyls and R₁R₂COO and H₂COO are the corresponding biradicals. Measured values of were 0.58–0.82 and indicate modest preferential formation of formaldehyde and the disubstituted biradical as compared to the ketone and the biradical H₂COO. Carbonyls other than the primary carbonyls were identified. Their formation is discussed in terms of subsequent reactions of the disubstituted biradicals R₁R₂COO. Similarities and differences between disubstituted and monosubstituted biradicals are outlined.  相似文献   

我国风云极轨气象卫星及应用进展     

何锡玉  蔡夕方  朱亚平  张雷 《气象科学进展》2021,(1)

我国风云极轨气象卫星数据,目前已经被应用在天气预报、气候预测、自然灾害、环境监测、科学研究等多个重要领域,为我国国民经济建设、国防建设、防灾减灾和全球许多国家的经济发展做出了重要贡献。本文介绍了我国风云极轨气象卫星的发展历程,重点阐述了目前风云极轨气象卫星在卫星数据预处理、定量产品反演、卫星资料同化等方面的应用研究进展,并展示了第二代风云极轨气象卫星在全球业务观测、大气三维探测、海洋环境监测、数值预报应用等相关典型应用。在国家创新战略发展框架下,根据“国家级气象现代化总体方案”确定的2020年气象卫星及应用发展目标,第二代极轨气象卫星风云三号03批卫星系统工程必将进一步发挥重大支撑作用。  相似文献   

Intercomparison of IRS-P4-MSMR derived geophysical products with DMSP-SSM/I and TRMM-TMI finished products     

A. K. Varma  R. M. Gairola  A. K. Mathur  B. S. Gohil  Vijay K. Agarwal 《Journal of Earth System Science》2002,111(3):247-256

In this paper, MSMR geophysical products like Integrated Water Vapour (IWV), Ocean Surface Wind Speed (OWS) and Cloud Liquid Water (CLW) in different grids of 50, 75 and 150 kms are compared with similar products available from other satellites like DMSP-SSM/I and TRMMTMI. MSMR derived IWV, OWS and CLW compare well with SSM/I and TMI finished products. Comparison of MSMR derived CLW with that derived from TMI and SSM/I is relatively in less agreement. This is possibly due to the use of 37 GHz in SSM/I and TMI that is highly sensitive to CLW, while 37 GHz channels are not available on MSMR. Monthly comparison of MSMR geophysical products with those from TMI is all carried out for climatological purpose. The monthly comparisons were much better compared to instantaneous comparisons. In this paper, details of the data analysis and comparison results are presented. The usefulness of the MSMR vis-à-vis other sensors is also discussed.  相似文献   

Alpine medicinal plant trade and Himalayan mountain livelihood strategies   总被引：5，自引：0，他引：5  

Carsten Smith Olsen  Helle Overgaard Larsen 《The Geographical journal》2003,169(3):243-254

Commercial alpine medicinal plants are collected from the wild by local rural households throughout the Himalaya and sold in order to increase household incomes. Recent studies indicate that this annual trade amounts to thousands of tonnes of roots, rhizomes, tubers, leaves, etc., worth millions of US dollars. The main market is in India. Based on a national survey, including the most commonly traded species, and a village study this paper investigates the importance of the alpine medicinal plant trade at national and local levels in Nepal. The national survey included standardized open-ended interviews with 232 harvesters, 64 local traders, 66 central wholesalers, 47 regional wholesalers, and 16 production companies. The village survey is based on the daily records of household activities in 15 households in a one-year period. The annual Nepalese alpine and sub-alpine medicinal plant trade is conservatively estimated to vary from 480 to 2500 t with a total harvester value of US$0.8–3.3 million; the average harvester value is estimated at US$66.0 ± 99.0. The trade in 1997/98 amounted to 1600 t with a harvester value of US$2.3 million and an export value equivalent to 2.5% of total export from Nepal. Medicinal plant harvesting was found to constitute an integrated part of local livelihood strategies, contributing from 3 to 44% (average of 12%) of the annual household income. Importance at household level depended on land and animal holdings, and the availability of adult male labour. The validity and reliability of methods and analyses is evaluated, and issues of conservation and management of alpine medicinal plant species under the community forestry scheme are discussed.  相似文献   

城市天气预报服务产品制作系统     

刘世祥  余勤  王小勇  何金梅  陈诚 《干旱气象》2003,21(2):12-14

利用FORTRAN和BASIC语言开发在WINDOWS．XX环境下运行的城市天气预报服务产品制作程序，包括报文采集、报文处理与翻译、报文检索与纠错、产品制作、产品输出与分发等内容，实现了大数量和多类型城市天气预报产品的自动制作。在保证准确、高效的专业预报服务中发挥了重要作用。  相似文献