Steroids from green alga Chaetomorpha basiretorsa Setchell     

史大永  范晓  孙杰  韩丽君  石建功 《中国海洋湖沼学报》2008,26(4)

Six steroids have been isolated from ethanolic extract of green alga Chaetomorpha basiretorsa Setehell by a combination of repeated normal phase silica gel and Sephadex LH-20 gel column chromatography as well as recrystallization. Using spectroscopic methods including MS and NMR, their structures were determined as β-lawsaritol (1), saringosterol (2), 24-hydroperoxy-24-vinyl-cholesterol (3), β-stigmasterol (4), stigmast-4-en-3α, 6β-diol (5), 29-hydroxystigmasta-5, 24 (28)-dien-3β-ol (6). All these compounds were obtained from this genus for the first time and they were inactive (IC5010 μg/ml) against KB, Bel-7402, PC-3M, Ketr 3 and MCF-7 cell lines.  相似文献   

凹顶藻属海藻化学成分研究进展   总被引：2，自引：0，他引：2  

史大永  贺?? 娟  许?? 凤  袁兆慧  范?? 晓  石建功  韩丽君 《海洋科学》2007,31(4):81-91

海洋生物通过生物链、化学信息传送、化学进攻、化学防御等机制相互依存、相对独立、维系着独特紧密的海洋生物体系,作为海洋物质代谢原动力的海藻为了对抗食藻动物的吞食,大多能产生一些有特色的代谢物质,其中凹顶藻属因富含萜类化合物著称,这类化合物含有异戊二烯结构单元,不  相似文献   

The chemical constituents from red alga Gymnogongrus flabelliformis Harv.     

袁兆慧  韩丽君  苏华  史大永  孙杰  李帅  石建功 《中国海洋湖沼学报》2008,26(2):190-192

Eight compounds were isolated from red alga Gymnogongrus flabelliformis Harv. In normal phase silica gel, Sephadex LH-20 gel column chromatography, reverse phase HPLC, and recrystallization. Based on MS and ID NMR spectroscopic data, their structures were determined as： stigmast-4-en-3-one （Ⅰ）, cholest-4-en-3-one （Ⅱ）, cholesterol （m）, uracil （Ⅳ）, uridine （V）, adenosine （Ⅵ）, succinic acid （Ⅶ）, and 5-hydroxy-4-methyl-5-pentyl-2,5-dihydro-furan-2-on （Ⅷ）. All of them were obtained from this species for the first time. Cytotoxicity of these compounds was screened using standard MTT method, but all the compounds were inactive （IC50 〉 10 μg/ml）.  相似文献   

海洋褐藻叉开网翼藻中的单萜成分研究     

徐秀丽  印丽媛  宋福行  范晓  石建功 《海洋科学》2012,36(10):81-84

为了研究中国沿海海藻中的活性化学成分, 利用正相硅胶柱色谱、Sephadex LH-20 柱色谱、反相HPLC 及重结晶等手段, 从采自青岛的褐藻叉开网翼藻(Dictyopteris divaricata Okam.)中首次分离得到了6 个单萜类化合物, 经红外波谱(IR), 质谱(MS)及核磁共振波谱(¹H NMR、¹³C NMR)等现代波谱技术鉴定为loliolide (1), isololiolide (2), 3β-hydroxy-5α,6α-epoxy-7-megastigmen-9-one (3), dehydrovomifoliol(4), (3R)-4-[(2R,4S)-2-hydroxy-2,6,6-trimethylcyclohexylidene]-3-buten-2-oneiol (5), (3R)-4-[(2R,4S)-4-acetoxy-2-hydroxy-2,6,6-trimethylcyclohexylidene]-3-buten-2-one (6)。并通过MTT 法对所得化合物进行细胞毒活性筛选, 通过比色法进行乙酰胆碱酯酶抑制活性筛选。  相似文献   

小粘膜藻4种化合物的分离和结构鉴定     

徐秀丽  范晓  宋福行  石建功 《海洋科学》2004,28(5):40-43

利用正相硅胶、生物胶Bio—beads、Sephadex LH-20柱色谱、反相HPLC及重结晶等分离手段,从小粘膜藻(Leathesia nana)中分离得到4个化合物,通过IR、MS、ID和2D NMR等现代波谱技术鉴定结构为：吲哚-3甲酸(1)、3,5-二经基-4-甲氧基苯甲醇(2)、1-O-十六烷酰基甘油酯(3)和岩藻甾醇(4)。以上化合物均为首次从该属海藻中分离鉴定。  相似文献   

Isolation and pharmacological activities of bromophenols from Rhodomela confervoides     

韩丽君徐年军  石建功严小军 《中国海洋湖沼学报》2005,23(2):226-229

Four bromophenols were isolated from the extract of marine red alga, Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods including IR, MS, 1D, and 2D-NMR techniques, their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) his (2,3-dibromo-4,5-dihydroxybenzyl) ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4)3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549 cells, 2 showed powerful antibacterial activities against Staphylococcus aurens and Pseudomonas aeruginosa.  相似文献   

褐藻小粘膜藻的化学成分     

徐秀丽  范晓  宋福行  杨永春  韩丽君  石建功 《海洋与湖沼》2005,36(1):18-23

应用正相硅胶、生物胶Bio-Beads、凝胶Sephadex LH-20柱色谱和反相HPLC等层析方法,从小粘膜藻(Leathesia nana)中分离得到7个化合物,通过IR、MS、1D和2DNMR等波谱技术鉴定为4-羟基苯甲酸(1)、3．羟乙酰基吲哚(2)、2,3-二溴．4,5-二羟基苯甲醛(3)、3-溴-4,5-二羟基苯甲醛(4)、2,3．二溴-4,5．二羟基苯甲基乙醚(5)、双-(2,3-二溴-4,5-二羟基卞基)-醚(6)和3．溴-4．(2,3-二溴-4,5-二羟基苯甲基)-5-甲氧甲基苯二酚(7)。  相似文献