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1.
ImODUcrroNTheVertchrateepidennisisinintirnatecontaCtwiththeenvironmentandre~environmentaladaptions.TheepidermisofterreStrialanbolsiskeratinedwhilethatofamPhibiousandaquaticfonnsisrelataltomucus-produCti0n.Theepidennisadsasafi-nalbatherbetweentheorganismanditsenvironmentandoneofitSrnainfunCtionsistoproteCttheorganismagainstextemalpathogens.lnadditiontoIneCanicalproedonofthebody,theepidertnisofamPhibiansandfishescansededinsandotherantibacterialpeptidesthathelpinthebonedefenSeoftheanhal(Su… 相似文献
2.
Amphioxus, Branchiostoma belcheri, a closest relative of vertebrates, is at a high risk of extinction due to a combination of low effective population size, altered native habitats and environmental pollution, yet little is known about cryopreservation of its gametes. This study deals with the cryopreservation of amphioxus semen. The main findings are that (1) the extender of Yao et al. is the best one among the four extenders examined; (2) the appropriate ratio of semen to extender of Yao et al. plus cryoprotectant is from 1:5 to 1:7; (3) dimethyl sulfoxide (DMSO) and methanol are the better cryoprotectants than glycerol, with DMSO giving the best results; (4) the eggs fertilized with post-thaw spermatozoa are capable of developing to at least hatching stage, and the highest hatching rate is (12.4±3.0)%. This is the first report on freezing and thawing of amphioxus spermatozoa, providing a simple and practical protocol for cryopreservation of amphioxus spermatozoa and laying a foundation for safeguarding this endangered species. 相似文献
3.
Pituitary gland, or pituitary for short, is characteristic of all vertebrates. As a “master gland” controlling a multitude of important functions in the body, its evolutionary origin has been an object of investigations of evolutionary biology for two centuries. Previous morphological, ultrastructural and immunohistochemical studies suggested the homology of the Hatschek’s pit of amphioxus and vertebrate pituitary. Developmental genetics study showed that the development of Hatschek’s pit and vertebrate pituitary is both subject to regulation by the common genes such as Pit1, Lhx3 and BMP3b. Our recent studies demonstrated that the Hatschek’s pit is able to secrete growth hormone (GH)-like hormone and thyroid-stimulating hormone (TSH)-like hormone that both play functions similar to vertebrate GH and TSH. We thus think that the emergence of Hatschek’s pit represents one of important events during endocrine network evolution, which laid a foundation for the subsequent formation of a hypothalamic-pituitary system in vertebrates. 相似文献
4.
Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively tested effects of A23187,
the calcium ionophore, on the development of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal
blastomeres committed to endoderm were treated with A23187 prior to gastrulation, they were transformed into ectodermal cells
as evidenced by the cell morphology and function characteristic of epidermis. However, the developmental fate of the same
blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged.
Thus, vegetal-endodermal cells in amphioxus embryos are not irreversibly determined before the gastrula stage, and artificial
increase in intracelluar Ca2+ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells.
Project 39470091 supported by NSFC and partly supported by Shandong Natural Science Foundation, Grant No.93D0140. 相似文献
5.
The pattern of resource allocation into multiple life‐history traits can vary with the environment; for example, high temperatures generally facilitate growth. Changes in investment in growth due to temperature may alter the allocation to other traits that are in trade‐off with that. Here, we investigated the trade‐off between growth and reproduction and whether the proportion of allocation to these varies with increasing seawater temperature in the lancelet Branchiostoma japonicum. We reared the lancelets of early adult stage under three different temperature treatments (control, + 1°C and + 2°C treatments) for 427 days in the laboratory. Although the total body length at initiation did not differ between treatments, the length was influenced by the interaction between elapsed days and temperature treatments, suggesting that growth rate was temperature‐dependent; the lancelets under higher temperature conditions showed a more rapid growth rate than did those under the control conditions. The frequency of individuals with gonads did not differ between treatments throughout their reproductive season. The monthly growth increments were also not significantly affected by the interaction between the presence/absence of gonads and temperature treatments, although the presence of gonads caused the growth increments to decrease. In addition, the effect of temperature treatments was significant. These results suggest that changes in temperature did not alter the pattern of allocation between growth and reproduction that were in a trade‐off, whereas high temperature would solely result in an increase in resource intake, at least in the early adult stage of this species. 相似文献
6.
The localization of nitric oxide synthases （NOS） is dealed with in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense. It was found by NADPH-diaphorase staining that （1） NOS activity was present in the nuclear membranes of germinal vesicles during the entire period ofoocyte development; （2） NOS was localized in both the nuclear membranes and the perinuclear region of cytoplasm in the vitellogenetic oocytes; （3） NOS was relocated in the cortical layer in the mature egg; （4） NOS activity was present in spermatocytes, but not in the spermatogonia in the middle of October; （5） NOS was detected in both spermatozoa and spermatids as well as spermatocytes during the breeding season. This is the first report on the distribution pattern of NOS in the developing gonads in protochordates. These results suggest a role for NOS in the functioning of the nuclear membranes and yolk synthesis during oogenesis and in cell division and differentiation during spermatogenesis. 相似文献
7.
厦门文昌鱼人工繁殖和幼虫发育及其变态的研究 总被引：6，自引：0，他引：6
用组织学和胚胎学方法对厦门国家级自然保护区文昌鱼的生殖季节、胚胎发育和幼虫培育进行详细研究,结果表明,自然保护区文昌鱼生殖季节可分为两个繁殖时期,从6月初开始至7月初为繁殖高峰期,8和9月为繁殖小产期.观察人工繁殖得出,文昌鱼(Branchiostoma belcheri)产卵时间在傍晚19:00前后,可以观察到雌、雄文昌鱼的产卵和排精行为:雄性文昌鱼先离开沙,并跃出水面快速游动和排精,接着雌鱼也跃出水面,所有的卵通过破裂的性腺壁进入到围鳃腔并通过围鳃孔到水中受精,受精率在98.5%以上,还观察了文昌鱼幼虫发育并变态为幼鱼(长度为1.05～13.5mm)的全过程. 相似文献
8.
Wu Xian-han Zhang Shi-cui Wang Yong-yuan Zhang Bao-lu Qu Yan-mei Jiang Xin-ji 《中国海洋湖沼学报》1994,12(4):289-294
Although amphioxus is widespread in temperate and tropical seas, its population is diminishing because of environmental pollution.
To keep the population of this evolutionarily important animal from diminishing, study on its reproduction and development
is necessary. The main findings in this study on the spawning and fecundity of the amphioxus reared in laboratory and its
larval development are as follows. 1. Water temperature markedly affected the spawning. It spawned only when water temperature
reached 21°C. 2. Spawning of the amphioxus in laboratory was markedly extended. Initially, the amphioxus spawned at about
7:00 PM, but spawning time was postponed as spawning days went on. 3. The number of eggs produced by a female ranged from
1400 to 12800, average of 5800. This also represents the fecundity of the amphioxus because it shedded all eggs within the
ovary at a time. 4. During the first few months of life of the amphioxus, its growth rate changed seasonally. The growth rate
in summer and fall was greater than that in winter. 5. The pelagic larva became a benthic adult after 50 days. 6. The amphioxus
reared in laboratory from fertilized eggs could produce fertile eggs and sperms. These findings can be a foundation for measures
to address the problem of diminishing amphioxus population.
Contribution No. 2274 from the Institute of Oceanology, Chinese Academy of Sciences
This work was supported by CNNSF, and Chinese Academy of Sciences. 相似文献
9.
本文根据2014年至2017年在厦门文昌鱼保护区黄厝、南线十八线、鳄鱼屿和小嶝岛四处潮下带以及参照点白城潮下带获得的大型底栖动物和沉积物粒径数据,探讨底质细化对厦门潮下带文昌鱼栖息地大型底栖动物群落的影响。聚类分析表明个别黏土含量较高的取样站没有发现文昌鱼且大型底栖动物种数少。单因素方差分析表明,厦门潮下带部分大型底栖动物群落参数和优势种数量存在显著差异。底质粒径分析表明, 2017年9月,鳄鱼屿和白城潮下带黏土和粉砂含量高于黄厝、南线十八线和小嶝岛潮下带的黏土和粉砂含量。2016年夏季黄厝、南线十八线潮下带黏土和粉砂含量明显高于2014年夏季的黏土和粉砂含量。相关分析表明,5个大型底栖动物群落参数如物种数、栖息密度、生物量、多样性指数(H′)和丰度指数(d), 7个常见种的栖息密度和生物量与粉砂和黏土含量呈显著正相关,均匀度指数(J)和4种常见种的数量与粉砂和黏土含量无显著相关,白氏文昌鱼(Branchiostoma belcheri)的栖息密度和生物量与粉砂和黏土含量呈显著负相关。上述结果证实,黏土含量增加到15%和粉砂含量增加到60%有利于多数大型底栖动物栖息密度和生物量的增加,但导致白氏文昌鱼栖息密度和生物量的减少,而红角沙蚕(Ceratonereiserythraeenis)、滑指矶沙蚕(Eunice indica)、光滑倍棘蛇尾(Amphioplus laevis)受底质细化的影响较小。 相似文献
10.
Mature amphioxus (Branchiostoma belcheri tsingtauense) collected by trawl in June, 1994 from Qingdao Shazikou sea area bottom sands, were cultured under controlled indoor conditions, where they spawned and their eggs were fertilized and hatched into larvae which gradually settled at the bottom and dug into the sands after 40-50 days culture in a water trough outdoors. The total survival rate of the larvae(LSR)was 5.5% before they went into the sands, 1.9% in 4 months, 0.7% in 10 months. The survival rate of the young fish which had dug into the sands (YSR) was 35.6% in 4 months, and 12.6% in 10 months. The amphioxus number tended to be constant from the 11th month on. In the first five months after incubation, the amphioxus body length increased by an average of about 1.5 mm a month, and about 0.4 mm a month from Dec. to May of next year. The maximum length after ten months was 24 mm; the average was about 11 mm. After two years culture in the water trough, the maximum length could reach 34-35 mm, when the amphioxus gonads began to develop. 相似文献