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1.
NK Arkert A-R Childs MC Parkinson AC Winkler E Butler S Mannheim 《African Journal of Marine Science》2018,40(3):235-244
Fisheries managers are increasingly promoting catch-and-release (C&R) to manage recreationally angled fish stocks. Despite this, there is a scarcity of information on the effects of C&R on estuarine-dependent species. Cape stumpnose Rhabdosargus holubi dominates the recreational fisheries catch and provides an important source of food for subsistence fishers in some temperate South African estuaries. The health and survival of R. holubi exposed to a C&R event was investigated by examining their physiological stress response (blood glucose and lactate), reflex impairment (reflex action mortality predictors [RAMP]) and short-term (12-hour) survival. Fish were captured and exposed to one of three air-exposure treatments: 0 s, 30 s or 90 s. Stress and health were measured either immediately (immediate) or one hour after (delayed) the C&R event. There was no significant difference in blood glucose between air-exposure treatments, but there was a significant difference between the mean immediate and delayed glucose levels within each treatment (F(2,143) = 81.8, p < 0.01). In contrast, blood lactate level was significantly higher in the 90-s treatment (p < 0.05). Immediate blood lactate levels were significantly lower than the delayed samples for each treatment (F = 4.29, p = 0.02; n = 169). Although all fish exhibited at least one reflex impairment, the RAMP score was significantly higher in the 90-s air-exposure treatment (H(2,86) = 9.73, p = 0.007). Also, RAMP scores were significantly lower in the delayed samples (p < 0.01). Although short-term mortality was relatively low (2.3%) for this species, it was highest in the 90-s treatment (7%). These results suggest that physiological stress is higher when R. holubi are exposed to longer periods of air exposure and that the physiological stress of fish subject to a C&R event is best measured after a delay. 相似文献
2.
目的:观察解郁安神法联合体外反搏(EECP)对经皮冠状动脉介入治疗(PCI)术后气滞血瘀型胸痹患者的临床疗效。方法:将80例冠心病PCI术后气滞血瘀型胸痹患者随机分为治疗组和对照组,每组各40例。对照组采用PCI术后规范化治疗,同时联合EECP治疗;治疗组在对照组基础上加服解郁安神方治疗,随访观察7周,比较2组临床证候疗效、Zung焦虑自评量表(SAS)评分、抑郁自评量表(SDS)评分、西雅图心绞痛调查量表(SAQ)评分。结果:总有效率治疗组为82.5%(33/40),高于对照组的60.0%(24/40),2组比较,差异有统计学意义(P<0.05);2组SAS、SDS、SAQ评分治疗前后组内比较及治疗后组间比较,差异均有统计学意义(P<0.05)。结论:解郁安神法联合EECP治疗可明显改善PCI术后气滞血瘀型胸痹患者的临床症状,缓解焦虑抑郁程度,提高患者生活质量,值得临床推广应用。 相似文献
3.
中国对虾血细胞酶细胞化学的初步研究 总被引:1,自引:0,他引:1
运用电镜酶细胞化学技术 ,对中国对虾 (Penaeus chinensis)的血细胞和血清中的酚氧化酶、酸性磷酸酶、碱性磷酸酶和过氧化物酶的酶活性部位进行标记。实验结果显示 :酚氧化酶主要以酶原形式存在于大颗粒细胞的颗粒体中 ,酸性磷酸酶和碱性磷酸酶以酶原形式存在于小颗粒细胞的颗粒体中。健康虾和患病虾血细胞中酶活性的表现形式不尽相同 ,在病理情况下 ,颗粒细胞能以细胞解体的方式 ,排出细胞内的颗粒 ,转化成有活性的酶 ,以此提高血淋巴中这几种酶的活性。透明细胞可以吞噬排放颗粒后的颗粒细胞 ,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。研究认为 :3种血细胞因含有不同的免疫酶以及酶的作用方式不同而具有不同的免疫功能 相似文献
4.
大连湾牡蛎的染色体数目 总被引:1,自引:0,他引:1
以大连湾牡蛎的早期胚胎为材料。经染色体数目分析,首次提出大连湾牡蛎的染色体数目为2n=20。为牡蛎遗传育种工作提供依据。 相似文献
5.
杆状病毒感染越冬亲虾(Penaeus chenesis)的研究——越冬亲虾感染及其垂直传播的可能性 总被引:2,自引:0,他引:2
在中国对虾越冬亲虾中检出杆状病毒。经对病虾鳃、前中肠、卵巢组织进行电镜观察,在这些组织中发现大量有囊膜的杆状病毒。病毒粒子大小约为367nm×128nm,核衣壳为324nm×92nm。病毒在细胞内不形成包涵体,在核内或在细胞质的发生基质中装配。从病毒的形态特征、感染组织以及典型的发病症状和病理变化,初步认为亲虾感染的病毒与近年广泛报道的造成养殖对虾大规模流行病的主要原因——中国对虾杆状病毒为同一种。卵巢组织也是病毒的主要靶器官之一,亲虾卵巢组织的被感染暗示着该病毒有可能通过生殖细胞而垂直传播 相似文献
6.
A strain of yeast, which can endure high osmotic pressure, is employed for the sensitive material of the microbial BOD sensor. Two immobilization methods are used, I.e. Calcium alginate gel be ads and PV A gel beads. The results show that the PVA gel beads is better. The influences of osmosis and heavy metal ions on the yeast entrapped in the PVA gel beads are also studied in the experiment. 相似文献
7.
扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用 总被引:9,自引:2,他引:9
采用噻唑盐(MTT)比色方法研究在紫外线辐射损伤条件下扇贝多肽对免疫细胞的保护作用,并探讨扇贝多肽对胸腺细胞和脾细胞活性的影响,结果表明,扇贝多肽具有抗紫外线氧化损伤的作用,可减轻或抑制紫外线对胸腺细胞和脾细胞的氧化损伤,并且呈剂量依赖性,扇贝多肽在0.5%-10.0%的浓度范围内,其抗氧化能力随浓度的增高而增强,在正常条件下可显著增强免疫细胞的活性,并且可拮抗雌激素对免疫细胞的抑制作用。提示扇贝多肽不仅具有抗氧化损伤作用,而且具有免疫增强使用。 相似文献
8.
Seng Chee Poh Norhaxati Mohd Tahir Hafiza Mohamed Zuki Mohd Izwadi Musa Khai Hock Ng Noor Azhar Mohamed Shazili 《中国地球化学学报》2006,25(B08):56-56
A total of 63 soil samples from 3 different soil profiles (urban, suburban and industrial areas) in major towns in the east coast of Peninsular Malaysia were analyzed for the total concentrations of Cu, Zn, Pb, Ni and Cr. The soil samples were subjected to acid digestion and the concentrations of total metals extracted were measured or flame atomic absorption spectrometry and inductively coupled plasma - atomic emission spectrometry. According to the result of this study, Pb and Zn concentrations in urban soils are much higher than those of industrial and suburban soils. Total concentrations of Cu and Cr in industrial soil samples are high compared to other two soil profiles and Ni concentrations in the suburban area are slightly higher those of urban and industrial soils. Since Malaysia has not yet to come up with her own soil maximum allowable limit, the heavy metal concentrations were compared with the Dutch maximum allowable limit. The results indicated that the median of heavy metals values in the three different soil profiles is still below the Dutch system limit. From the maximum allowable value obtained from the Dutch system, a contamination/pollution (C/p) index for each site was calculated for the set of these five heavy metals. An advantage of using this method is to make a differentiation between pollution (C/p〉1) and contamination (C/p〈1) status in soils as well as being able to characterize each status into 5 different categories (slight, moderate, severe, very severe and excessive). 相似文献
9.
Brian Gulson Karen Mizon Michael Korsch Honway Louie Michael Wu Jenny Stauber J. Michael Davis Alan Taylor 《中国地球化学学报》2006,25(B08):62-62
This is a 4-year longitudinal study to evaluate changes to the environment and exposure of young children associated with the introduction of methylcyclopentadienyl manganese tricarbonyl (MMT) into Australia in 2000. The cohort includes 57 females and 56 males; age range of 0.29 to 3.9 years. Samples are collected every 6 months from children in residences located at varying distances from major traffic thoroughfares in Sydney. Environmental samples: air, house and day care dustfall, soil, dust sweepings and gasoline. Samples from children: blood, urine, handwipes prior to and after playing outdoors, and a 6-day duplicate diet. All samples are analyzed for a suite of 20 elements using ICP-MS. Results are presented for the first three 6-month sampling periods for Pb and Mn. For dustfall accumulation, there was no significant change over the 3 sampling periods (time) for Pb or Mn, and a positive relationship between ‘traffic exposure' (traffic volume and proximity to the road) and Pb but not Mn. For handwipes, Pb and Mn in wipes taken from children after playing outdoors were usually significantly greater than for wipes taken prior to playing. There was no significant association between Pb or Mn in handwipes with traffic exposure. Dustfall accumulation was a significant predictor for Pb in the handwipes, and dust sweepings were a significant predictor of Mn in handwipes. 相似文献
10.
p-nitrotoluene is an important organic intermediate widely used in pesticide, foamed plastics, dyestuff and medicine industries.
In this paper, Crusian carps (Carassius auratus) were exposed to dilute p-nitrotoluene solutions with different concentrations of 0, 20, 40, 80, 120, 160, 240 and 320 μg/L; the subsequent physiological
responses to the chemical were observed, and the variation characteristics of blood corpuscles, ovaries and oocytes were studied
by bio-microscopy. Results showed that even very low concentrations of p-nitrotoluene would lead to toxicosis and even the death of Carassius auratus. The bio-microscopic structures revealed that, compared with the compartment of Carassius auratus growing in the p-nitrotoluene-free water (the control group), the lower concentrations of p-nitrotoluene (for example no more than 80 μg/L) would result in the faster disintegration of erythrocytes and the significant
enlargement of oocytes, while the higher contents of p-nitrotoluene (no less than 240 μg/L in this case) would inhibit the division of cells and their further growth, even degenerate
blood corpuscles and ovaries, suggesting stronger estrogenic activities.
The study is financially supported by the Development Foundation of Science and Technology under Shandong Provincial Education
Department (No. J05D51). 相似文献