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1.
The localization of nitric oxide synthases (NOS) is dealed with in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense. It was found by NADPH-diaphorase staining that (1) NOS activity was present in the nuclear membranes of germinal vesicles during the entire period ofoocyte development; (2) NOS was localized in both the nuclear membranes and the perinuclear region of cytoplasm in the vitellogenetic oocytes; (3) NOS was relocated in the cortical layer in the mature egg; (4) NOS activity was present in spermatocytes, but not in the spermatogonia in the middle of October; (5) NOS was detected in both spermatozoa and spermatids as well as spermatocytes during the breeding season. This is the first report on the distribution pattern of NOS in the developing gonads in protochordates. These results suggest a role for NOS in the functioning of the nuclear membranes and yolk synthesis during oogenesis and in cell division and differentiation during spermatogenesis.  相似文献   
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南海黄鲷性腺发育的初步研究   总被引:1,自引:0,他引:1  
本文采用常规的生物学调查方法并取黄鲷性腺做石蜡切片,用显微镜观察黄鲷的性腺发育,进行组织学研究.其结果表明,南海黄鲷精巢可分成6期,精巢5期的成熟度指数为1.62%~2.71%,平均值为1.99%.雌性生殖细胞可见5个时相,卵巢发育也相应分成6期,卵巢5期时的成熟度指数为8.90%~13.90%,平均值为9.45%.南海黄鲷的繁殖期在11月至翌年的4月,繁殖高峰在12月至3月,黄鲷的产卵类型属于在同一繁殖季节中多次产卵类型.本文还对黄鲷的雌雄同体现象、繁殖力、繁殖时间进行了讨论.  相似文献   
3.
To clarify the differences in the growth and gonad size of the sea urchin Hemicentrotus pulcherrimus among algal sere, the study was conducted in June and July, and September 1998 at three fucoid beds in Oga and at three algal turfs in Hachimori, Akita Prefecture in northern Japan. The most rapid growth was observed in a large perennial fucoid bed at the climax stage. Growth in a small perennial Chondrus ocellatus‐dominated bed in algal turfs in the seral stage was then high. The slowest growth was observed in the small perennial Dictyopteris divaricata‐ and Laurencia spp.‐dominated beds which are known to possess chemicals which act as feeding deterrents against the sea urchins. The gonad index (gonad wet weight × 100/body wet weight) at a fucoid bed was high where standing crops exceeded 3 kg·m?2. The gonad index in the Laurencia bed was lower than those at fucoid beds. These results suggest that growth and gonad production are affected by algal sere and differ among species of small perennial algae in the seral stage with or without chemical defense.  相似文献   
4.
根据对青岛附近水域欧氏六线鱼(已达性成熟年龄)性腺周年宏观和组织学观察,性腺发育可分为:①重复发育Ⅱ期;②开始成熟期;③接近成熟期;④临产期或产卵期;⑤产后期。按性腺指数变化并结合性腺组织切片确定,欧氏六线鱼性腺发育在青岛海区一年一个周期,繁殖期在10月下旬至12月,繁殖盛期是11月下旬至12月中旬。  相似文献   
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Aspects of the reproductive biology of the brown mussel Perna perna at the Iture rocky beach near Cape Coast, Ghana, were studied from September 2014 to August 2015. The current study was aimed at providing information useful for managing the mussel fishery in this locality and also that would form the basis for designing appropriate culture methods for the species. Microscopic examination of fresh smears of gonadal material, as well as histological preparations of the gonad, were used to study the sexuality and breeding pattern of the species. Monthly gonadal and condition indices were also determined. Perna perna exhibited gonochoristic sexuality with a sex ratio of approximately 1:1 throughout the study period. Sexes were identifiable at shell lengths of 15.0–19.9 mm. Five stages of gonadal development were identified in both sexes. Gametogenic activity was continuous throughout the year, with two major spawning activities, from April to June and from August to December. These periods coincided with the major and minor rainy seasons, respectively, as well as the major upwelling period in August. Condition indices suggest that the mussels were in better condition for harvesting in March and August prior to the major spawning events.  相似文献   
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李应东  孙世春 《海洋与湖沼》2014,45(6):1353-1358
本文研究了去头术和外源褪黑激素对香港细首纽虫(Cephalothrix hongkongiensis)性腺发育的影响,并对褪黑激素合成酶HIOMT进行了Western blot测定和免疫组织化学定位。结果表明,去除头部可快速诱导香港细首纽虫的性腺发育,处理40天后配子完全成熟,而对照组(完整个体)在整个实验过程中均未发现性腺。这说明香港细首纽虫头部具有抑制性腺发育的物质(激素)。外源褪黑激素对香港细首纽虫的性腺发育有显著的抑制作用,并且随着浓度的升高,抑制作用增强。Western bolt结果显示,在38k Da处有阳性条带;免疫组织化学定位发现,脑神经节的几个神经细胞有阳性反应,说明香港细首纽虫的脑神经节中存在HIOMT,具有合成褪黑激素的功能。  相似文献   
9.
青岛文昌鱼(Branchiostoma japonicus)采自于青岛沙子口海区,在室内进行周年培育。每月采集样品,经测量、固定以及组织切片观察,研究了其周年卵巢和精巢的发育规律。结果表明,室内培育的文昌鱼一周年内全长没有明显变化,仅体质量在繁殖期即其性腺发育至Ⅴ期时有明显增大(P0.05=,精卵排空后则恢复原来体质量。文昌鱼1 a内在夏季6~7月繁殖1次,能自行排放卵和精子,排空后个体的卵巢或精巢,在适宜的条件下,经历Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ期的发育过程,进行下一轮的性腺发育。文昌鱼精巢在Ⅳ期前先于卵巢发育,两者在Ⅳ期前发育都不同步,精巢处于Ⅳ期时间较长,一般从3月持续到5月,而卵巢处于Ⅳ期一般是在4月~5月。  相似文献   
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