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本文以~3H—TdR掺入法观察911对小鼠脾淋巴细胞增殖反应的影响并用CTLL细胞检测了其对IL—2的作用。体外实验结果表明,911对小鼠脾淋巴细胞增殖反应有明显的增强作用,以0.5μg/ml的浓度效果最为明显,相对增殖指数RPI可达200%;体内实验则以5mg/kg体重ip,连续7d效果最好,相对增殖指数RPI可达176%;911用药组小鼠IL—2的产生量均高于对照组,以5mg/kg和10mg/kg效果最好。以上实验证明,这一多糖是一种有希望的新免疫调节药物。 相似文献
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To study immune mechanism of fish lymphocyte we performed a proliferation assay and ELISA using monoclonal antibody against
human IL-2. The result showed that an interleukin-2 (IL-2)-like factor was detected in the supernatant of plant haemoglutinin
(PHA)-stimulated lymphocyte culture from peripheral blood, spleen and head kidney of olive flounder,Paralichthys olivaceus. The quantities of IL-2-like factor in the supernatant from different lymphoid tissues were quite different. The IL-2 like
factor in the supernatant from cultured head kidney lymphocytes was much higher than those of peripheral blood lymphocytes
and spleen lymphocytes (P<0.01). The IL-2 activity was found in either mouse thymocyte proliferation assay or flounder head kidney lymphocyte proliferation
assay and shown to have obvious enhancing effect on proliferation of the above two types of cell. The recombinant human IL-2,
(rhIL-2) was able to stimulate flounder thymocyte proliferation and used to detect the IL-2 receptor (IL-2R) on the surface
of flounder lymphocyte. The cross-reaction between the lymphocytes of flounder peripheral blood and CD25(IL-2R) was detected
with flow cytometry and shown that the percentage of CD25-positive cell in peripheral blood was 7.74±0.67%.
This work was supported by National “973” Project G1999012003, G19999012006. 相似文献
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碱性成纤维细胞生长因子与胰岛素样生长因子Ⅱ对中国对虾淋巴细胞培养物的协同诱导作用 总被引:2,自引:0,他引:2
对虾的传染性疾病是限制对虾养殖业发展的主要因素.目前至少发现了17种对虾病毒病并鉴定出了30多种对虾病毒[1~5].虽然已在其宿主动物---虾中对对虾病毒病进行了多方面的研究,但为了分离、纯化和鉴定这些病毒,必须建立一个适宜于对虾组织的体外培养系统.繁殖并弄清病毒的分子结构及其理化特性是研究病毒病、检测和治疗病毒病的重要手段,而发展对虾细胞的体外培养和建系技术是解决这些问题的关键之一. 相似文献
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In order to provide pertinent evidence of ion channel with immune response in the fish,whole cell patch-clamp technique was employed for potassium ion channel study in turbot(Scophthalmus maximus) . Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples,and electrophysiological characters of the channel were analyzed. In the recorded cells,activated voltage of the channels was-42.5 ± 3.7 mV and the average peak current was 313.12 ± 28.2 pA. The channel was identified as voltage dependent,the current was outward and it could be inhibited by 10 mmol/dm 3 TEA or 5 mmol/dm 3 4-AP,a specific potassium channel inhibitor,identifying the existence of potassium channel in peripheral lymphocytes of the turbot. 相似文献
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革皮氏海参皂苷对小鼠免疫功能的调节作用 总被引:9,自引:0,他引:9
从革皮氏海参(Pearsonothuria graeffei,P.graeffei)中提取海参皂苷,并探讨其对正常小鼠和免疫功能低下小鼠免疫功能的影响。结果表明,对于正常小鼠,革皮氏海参皂苷能显著提高小鼠的细胞免疫和非特异性免疫功能,显著增加小鼠的足跖增厚值(P0.05),促进腹腔巨噬细胞的吞噬能力(P0.05)。对于免疫功能低下小鼠,革皮氏海参皂苷能显著提高小鼠的抗体形成细胞数和小鼠血清溶血素含量(P0.05,P0.01),促进小鼠的体液免疫功能;显著促进小鼠的迟发型变态反应,提高小鼠脾淋巴细胞的增殖能力(P0.01),提高小鼠的细胞免疫功能;显著促进小鼠腹腔巨噬细胞对鸡红细胞的吞噬率和吞噬指数(P0.01),促进小鼠的非特异性免疫功能。提示革皮氏海参皂苷对小鼠具有显著的免疫调节作用。 相似文献
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