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1.
Characterization of a Mutant of Alteromonas aurantia A18 and Its Application in Mariculture 总被引：1，自引：0，他引：1
Mutant J61321 with enhanced siderophore production of Alteromonas aurantia AI8 was obtained after a series of chemical-physical mutageneses. It was found that the antibacterial activity against Vibrio anguillarum W-1 and siderophore production of the mutant were higher than those by the original strain A 18 which had been used in mariculture. The results of the specific assay（Csaky and Arnow methods） of siderophore showed that the sidrophore with hydroxamate group was produced by mutant J61321 and the original strain A 18, respectively, while the siderophore with catechol group was yielded by strain W-1 （Aibrio anguillarum）. Meanwhile, the siderophore yield, antibacterial activity and anti-chelator activity of strain J61321 were higher than those of its parent strain A 18. 相似文献
2.
Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio 总被引：1，自引：0，他引：1
Zebrafish （Danio rerio） genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine, while the late development （organogenesis） was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail, wavy body, golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures, curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil-and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control, suggesting that most mutagenesis induced by these agents are point mutations. However, some fish derived from 5-bromouracil-treated embryos had golden（gol） pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control.Possibly, 5-bromouracil can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help for detailed studies of mutagenesis. 相似文献
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经紫外诱变,筛选出在碳酸氢铵浓度为400 mg/L条件下可以生存的突变株。通过培养基中氮、磷、铁、温度、光照及pH值对雨生红球藻诱变株生长的影响进行比较,对以上3种营养盐及3种环境因子分别进行三因素三水平正交实验,以优化培养条件。进而对雨生红球藻野生型及诱变株的生长状况及色素含量进行比较。实验结果表明,雨生红球藻抗铵品系在培养基中NaNO30.1 g/L,KH2PO40.01 g/L,FeCl3.6H2O 1.0 mg/L,在pH值为8.0,光照强度100μmol.m-2.s-1,温度为18℃的培养条件下,雨生红球藻的生物量得到有效提高。 相似文献
5.
利用紫外诱变法对三角褐指藻(Phaeodactylum tricornutum)进行诱变育种。实验得到三角褐指藻的最佳紫外辐射剂量为18 W的紫外灯距离藻液35 cm照射15 min。通过单细胞分离技术获得1株突变株UP1,与出发藻株相比,突变株UP1的EPA产量提高10.2%。研究了诱变株的最适生长及产EPA的条件,结果表明UP1在NaNO_3 75 mg/L,p H 7.5,昼夜温度17~15℃,接种量为10%时培养7天,具有最大的生长速率和EPA产量。探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传。 相似文献
6.
深海微生物由于长期生活在高压、低温、黑暗、寡营养的极端环境中,必然会发展出与之相应的分子基础。近年来,对深海微生物极端环境适应性机理的研究是深海微生物研究的前沿课题。通过对一株耐冷、耐压的深海沉积微杆菌Microbacterium sediminis YLB-01进行ARTP诱变,成功获得了对低温和高压敏感的突变子9-3E5,突变子9-3E5与野生型菌株的基因组比较分析结果表明,SNP和In Del在突变子9-3E5全基因组上分布均匀,838个SNP位点位于编码区且为非同义突变,占总SNP数量的77.45%; 27个In Del中11个位于编码区且发生了移码突变;突变子9-3E5共有11条SV序列,长度为300～400 bp的序列占比为81.82%。以OR值 2、p 0.05为标准对突变基因进行富集,共富集到3个相关的功能集。在与细胞分裂相关的蛋白中发现了11个SNP突变位点,主要涉及细胞分裂蛋白Fts I、Fts W、Fts X和细胞分裂起始蛋白;在与DNA修复、合成相关的6个基因中发现了10个SNP突变位点;在与胞内运输和能量合成调控相关的2个基因中发现了3个SNP突变位点。我们推测细胞分裂、DNA损伤修复及合成、胞内运输对能量合成的影响这3类功能基因发生改变可能是突变子9-3E5相对于野生株YLB-01低温、高压耐受性改变的原因,M.sediminis YLB-01通过调节这些胞内的生理生化过程来应对外界低温和高压环境。 相似文献
7.
Zebrafish (Danio rerio) genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil
and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development
of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine, while the late development (organogenesis)
was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail, wavy body, golden pigmentation and
the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures, curled tails and wavy
body. RAPD analysis showed that the majority of 5-bromouracil- and 6-aminopurine-treated larvae and fish shared banding patterns
in common with the control, suggesting that most mutagenesis induced by these agents are point mutations. However, some fish
derived from 5-bromouracil-treated embryos had golden (gol) pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control. Possibly, 5-bromouracil
can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help
for detailed studies of mutagenesis.
This work was supported by Ministry of Science and Technology (MOST) of China (G 1999012005). 相似文献
8.
用紫外线辐射处理野生型龙须菜果孢子体放散刚附着的果孢子,并通过耐高温的筛选,初步获得了2株具有耐高温,生长快速等优良性状的四分孢子体突变株,命名为BZT-11和BZT-18,将其与981和野生型藻株进行了比较分析。结果显示,这2株突变体在实验室内和海上的日平均生长速率为均明显高于981和野生型藻株。在热胁迫时间内,BZT-11和BZT-18的丙二醛(MDA)含量均明显低于野生型,超氧化物歧化酶(SOD)活性明显高于野生型;而在整个胁迫过程中2株突变体与981藻株的MDA含量和SOD活性水平差异不显著。经过4h的热激后,藻株BZT-11和BZT-18的hsp70基因表达水平也显著高于野生型。这些结果进一步证明了该2株突变藻体的耐高温优势。本研究提供了耐高温突变体的筛选技术。 相似文献
9.
Hyu：n-Jeong Jeong Hyoungseok Leet Soon Gyu Hong Jang-Cheon Cho Hong Kum Lee Yoo Kyung lee 《极地研究(英文版)》2013,(4):223-230
Random mutagenesis is commonly used to study gene function. The screening of mutants exhibiting specific pheno- types assists in the identification of phenotype-related genes. In the current study, we isolated Antarctic bacteria, and developed a transposon Tn5 mutagenesis system. A total of 26 strains were isolated from seawater and freshwater near Antarctic King Sejong Research Station, King George Island. Six Psychrobacter strains were identified as psychrophilic, with optimal growth tempera- tures of 10~C or 15~C Psychrobacter cryohalolentis PAMC 21807 with a high growth rate at 4~C was selected for transposon mutagenesis. Tri-parental conjugation with a plasmid containing Tn5 produced 13 putative recombinants containing the selectable marker. Genomic Southern hybridization confirmed Tn5 existed as episomes for seven recombinants, and for a single recombinant, Tn5 was integrated into the genome of Psychrobacter cryohalolentis PAMC 21807. The result indicates that the mutagenesis method, although successful, has a relatively low rate. The psychrophilic bacteria isolated in this study may be a useful resource for studying cold adaptation mechanisms, and the mutagenesis method can be applied to genetic analysis. 相似文献
10.
Constantine Uwaremwe ShiWeng Li XiMing Chen Maurice Ngabire Tawheed Mohammed Elheesin Shareef Juan Li MingHui Wu GuoLi Li 《寒旱区科学》2017,9(2):167-174
In this study, an Arthrobacter strain from desert soils in the Shule River Valley was isolated, China, which has a strong ability to convert cellulose to potential biofuel. In total, from five soil sample sites, six strains were isolated that grew well on CMC-Agar medium, with colony diameters ranging from 3~4 mm, among them, one strain had a strong ability to produce biofuels. Based on morphological and phylogenetic analyses, the isolate was identified as Arthrobacter nitroguajacolicus strain SLP1. The chemical properties of the biofuel extracted from the fermentation broth of strain SLP1 were analysed by gas chromatography and mass spectrometry. A total of 41 kinds of carbon compounds were identified, of those,five were detected at peak concentration and the carbon numbers ranged from C16–C22, which can be classified as alkanes,alkenes, and alcohols. Furthermore, biofuel-producing ability of strain SLP1 was enhanced using NTG mutagen. In a total of 94 mutant strains, four show the most enhanced biofuel production relative to the original strain. Biofuel production conditions were optimized by growing the four mutant strains on LB-Agar and SS-Agar medium. 相似文献