采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。 相似文献
Considering the current disadvantages of present offshore wind turbine foundations, a novel anchor foundation with skirt and branches is proposed, called offshore umbrella suction anchor foundation (USAF). A series of experiments and numerical simulations were performed to explore the bearing capacity of the USAF under various kinds of loading modes. The bearing characteristics and the anchor–soil interactions are described in detail for horizontal static loading, horizontal cyclic loading, and an antidrawing (pullout) test in silty soil. In the static loading test, the load–deflection of the anchor under step loading was analyzed and the normalized curve of the load–deflection was obtained to determine the ultimate horizontal bearing capacity of the anchor under normal working conditions. Under horizontal cyclic loading, the relationship between the plastic cumulative deformation and cyclic number was determined. In addition, the responses of USAF were investigated for a low wave frequency and storm surges. In the drawing test, it was found that a “segmentation phenomenon” occurred during the test. Moreover, a method to identify the maximum antidrawing load of USAF was provided based on dynamic mechanics. The numerical results show that the use of anchor branches and skirt can enhance the bearing performance of USAF to a certain degree. However, the anchor branch has a slight positive influence on the bearing performance improvement. The USAF is not only similar to a stiff short pile, but a rotation occurs. The failure envelope under composite loading (V-M) was obtained and the changes associated with changes in the aspect ratio of the internal compartment were clarified. 相似文献
Atlantic salmon reared in recirculating aquaculture system (RAS) may lead to inappropriately high stocking density, because fish live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the influence of stocking density on growth and some stress related physiological factors were investigated to evaluate the effects of stocking density. The fish were reared for 220 days at five densities (A: 24 kg/m3; B: 21 kg/m3; C: 15 kg/m3; D: 9 kg/ m3 and E: 6 kg/m3 ). The results show that 30 kg/m3 might be the maximum density which RAS can afford in China. The stocking densities under 30 kg/m3 have no effect on mortality of Atlantic salmon reared in RAS. However, the specific growth rate (SGR), final weight and weight gain in the high density group were significantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate (FCR) had a negative correlation with density. Plasma hormone T3 and GH showed significant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone (T3), GH (growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT (glutamic oxalacetic transaminase) and GPT (glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no effects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise efficient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.