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2.
Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels 总被引:5,自引:0,他引:5
Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured. 相似文献
3.
The microbial transformation of typical tar oil compounds such as acridine, benzo(b)thiophene, dibenzofuran, indane, and indene under different redox conditions was investigated in microcosm studies. Under aerobic conditions the inherent contamination in polluted soil as well as the added N‐, S‐, O‐ heterocyclic and homocyclic compounds were transformed predominantly apart from thiophene. 1‐Indanone was detected by RP‐HPLC‐DAD and GC‐MS as an intermediate metabolite from indane and indene. Under nitrate and sulfate reducing conditions indane, benzo(b)thiophene, and dibenzofuran were transformed in assays with the polluted soil samples from well B 65 and B 66 within 426 days, whereas they were persistent in assays with the highly polluted soil B 67. All heterocyclic and homocyclic compounds added to the non‐contaminated soil from well B 85 were not degraded under nitrate and sulfate reducing conditions, too. The results indicate that for the decision, wether natural attenuation can be implemented in the remediation of contaminated site, in addition to BTEX and PAHs especially the fate of further tar oil compounds in anoxic aquifers has to be considered. 相似文献
4.
Joseph M. Delesantro Jonathan M. Duncan Diego Riveros-Iregui Joanna R. Blaszczak Emily S. Bernhardt Dean L. Urban Lawrence E. Band 《水文研究》2021,35(9):e14339
Current land-use classifications used to assess urbanization effects on stream water quality date back to the 1980s when limited information was available to characterize watershed attributes that mediate non-point source pollution. With high resolution remote sensing and widely used GIS tools, there has been a vast increase in the availability and precision of geospatial data of built environments. In this study, we leverage geospatial data to expand the characterization of developed landscapes and create a typology that allows us to better understand the impact of complex developed landscapes across the rural to urban gradient. We assess the ability of the developed landscape typology to reveal patterns in stream water chemistry previously undetected by traditional land-cover based classification. We examine the distribution of land-cover, infrastructure, topography and geology across 3876 National Hydrography Dataset Plus catchments in the Piedmont region of North Carolina, USA. From this dataset, we generate metrics to evaluate the abundance, density and position of landscape features relative to streams, catchment outlets and topographic wetness metrics. While impervious surfaces are a key distinguishing feature of the urban landscape, sanitary infrastructure, population density and geology are better predictors of baseflow stream water chemistry. Unsupervised clustering was used to generate a distinct developed landscape typology based on the expanded, high-resolution landscape feature information. Using stream chemistry data from 37 developed headwater catchments, we compared the baseflow water chemistry grouped by traditional land-cover based classes of urbanization (rural, low, medium and high density) to our composition and structure-based classification (a nine-class typology). The typology based on 22 metrics of developed landscape composition and structure explained over 50% of the variation in NO3−-N, TDN, DOC, Cl−, and Br− concentration, while the ISC-based classification only significantly explained 23% of the variation in TDN. These results demonstrate the importance of infrastructure, population and geology in defining developed landscapes and improving discrete classes for water management. 相似文献
5.
Basin evolution and palaeoenvironmental variability of the thermokarst lake El'gene‐Kyuele,Arctic Siberia 下载免费PDF全文
Philipp Schleusner Boris K. Biskaborn Frank Kienast Juliane Wolter Dmitry Subetto Bernhard Diekmann 《Boreas: An International Journal of Quaternary Research》2015,44(1):216-229
Thermokarst lakes are a widespread feature of the Arctic tundra, in which highly dynamic processes are closely connected with current and past climate changes. We investigated late Quaternary sediment dynamics, basin and shoreline evolution, and environmental interrelations of Lake El'gene‐Kyuele in the NE Siberian Arctic (latitude 71°17′N, longitude 125°34′E). The water‐body displays thaw‐lake characteristics cutting into both Pleistocene Ice Complex and Holocene alas sediments. Our methods are based on grain size distribution, mineralogical composition, TOC/N ratio, stable carbon isotopes and the analysis of plant macrofossils from a 3.5‐m sediment profile at the modern eastern lake shore. Our results show two main sources for sediments in the lake basin: terrigenous diamicton supplied from thermokarst slopes and the lake shore, and lacustrine detritus that has mainly settled in the deep lake basin. The lake and its adjacent thermokarst basin rapidly expanded during the early Holocene. This climatically warmer than today period was characterized by forest or forest tundra vegetation composed of larches, birch trees and shrubs. Woodlands of both the HTM and the Late Pleistocene were affected by fire, which potentially triggered the initiation of thermokarst processes resulting later in lake formation and expansion. The maximum lake depth at the study site and the lowest limnic bioproductivity occurred during the longest time interval of ~7 ka starting in the Holocene Thermal Maximum and lasting throughout the progressively cooler Neoglacial, whereas partial drainage and an extensive shift of the lake shoreline occurred ~0.9 cal. ka BP. Correspondingly, this study discusses different climatic and environmental drivers for the dynamics of a thermokarst basin. 相似文献
6.
Contemporary Deposition and Long-Term Accumulation of Sediment and Nutrients by Tidal Freshwater Forested Wetlands Impacted by Sea Level Rise 总被引:1,自引:0,他引:1
Gregory?B.?NoeEmail author Cliff?R.?Hupp Christopher?E.?Bernhardt Ken?W.?Krauss 《Estuaries and Coasts》2016,39(4):1006-1019
Contemporary deposition (artificial marker horizon, 3.5 years) and long-term accumulation rates (210Pb profiles, ~150 years) of sediment and associated carbon (C), nitrogen (N), and phosphorus (P) were measured in wetlands along the tidal Savannah and Waccamaw rivers in the southeastern USA. Four sites along each river spanned an upstream-to-downstream salinification gradient, from upriver tidal freshwater forested wetland (TFFW), through moderately and highly salt-impacted forested wetlands, to oligohaline marsh downriver. Contemporary deposition rates (sediment, C, N, and P) were greatest in oligohaline marsh and lowest in TFFW along both rivers. Greater rates of deposition in oligohaline and salt-stressed forested wetlands were associated with a shift to greater clay and metal content that is likely associated with a change from low availability of watershed-derived sediment to TFFW and to greater availability of a coastal sediment source to oligohaline wetlands. Long-term accumulation rates along the Waccamaw River had the opposite spatial pattern compared to contemporary deposition, with greater rates in TFFW that declined to oligohaline marsh. Long-term sediment and elemental mass accumulation rates also were 3–9× lower than contemporary deposition rates. In comparison to other studies, sediment and associated nutrient accumulation in TFFW are lower than downriver/estuarine freshwater, oligohaline, and salt marshes, suggesting a reduced capacity for surface sedimentation (short-term) as well as shallow soil processes (long-term sedimentation) to offset sea level rise in TFFW. Nonetheless, their potentially large spatial extent suggests that TFFW have a large impact on the transport and fate of sediment and nutrients in tidal rivers and estuaries. 相似文献
7.
Geographic Information Systems (GIS) are well suited to support environmental modeling for dealing with space. However, some of the limitations of current GIS are the lack of tools for comprehensive documentation of the models, the inadequate representation of fields, and the deficient methodology for comprehensive management of uncertainty. Using Digital Terrain Modeling and Analysis as an example, this paper proposes an enhanced approach to overcome these drawbacks. It suggests implementing sophisticated functionality for modeling and analysis of fields in special-purpose modules outside monolithic GIS. These modules include three components: (1) an extensive framework for metainformation that allows a sound assessment of the fitness-for-use of digital field representations for environmental modeling applications, (2) an explicit digital representation of the field phenomenon equipped with the appropriate tools for the derivation of data, and (3) the methods to assess the quality of derived data. A standardized interface enables communication between the module and other software components. The presented modular approach combines the functionality of common GIS with highly specialized modeling and analysis tools encapsulating expert knowledge about the represented phenomena. 相似文献
8.
Jérôme Gattacceca Francis M. McCubbin Jeffrey N. Grossman Devin L. Schrader Nancy L. Chabot Massimo D'Orazio Cyrena Goodrich Ansgar Greshake Juliane Gross Katherine Helen Joy Mutsumi Komatsu Bingkui Miao 《Meteoritics & planetary science》2023,58(6):901-904
Meteoritical Bulletin 111 contains the 3094 meteorites approved by the Nomenclature Committee of the Meteoritical Society in 2022. It includes 11 falls (Antonin, Botohilitano, Cranfield, Golden, Great Salt Lake, Longde, Msied, Ponggo, Qiquanhu, Tiglit, Traspena), with 2533 ordinary chondrites, 165 HED, 123 carbonaceous chondrites (including 4 ungrouped), 82 lunar meteorites, 28 Rumuruti chondrites, 27 iron meteorites, 23 ureilites, 22 mesosiderites, 22 Martian meteorites, 21 primitive achondrites (one ungrouped), 17 ungrouped achondrites, 13 pallasites, 7 enstatite achondrites, 6 enstatite chondrites, and 5 angrites. Of the meteorites classified in 2022, 1787 were from Antarctica, 1078 from Africa, 180 from South America, 34 from Asia, 6 from North America, 4 from Europe, and 1 from Oceania. 相似文献
9.
Juliane Bernhardt Christof Engelhardt Georgiy Kirillin J?rg Matschullat 《Climatic change》2012,112(3-4):791-817
Rising northern hemispheric mean air temperatures reduce the amount of winter lake ice. These changes in lake ice cover must be understood in terms of resulting effects on lake ecosystems. Accurate predictions of lake ice phenology are essential to assess resulting impact. We applied the one-dimensional physical lake model FLake to analyse past variability in ice cover timing, intensity and duration of Berlin-Brandenburg lakes. The observed ice phenology in two lakes in the period 1961–2007 was reconstructed by FLake reasonably well and with higher accuracy than by state-of-the-art linear regression models. Additional modelling results of FLake for 38 Berlin-Brandenburg lakes, observed in the winter of 2008/09, were quite satisfactory and adequately reproduced the effects of varying lake morphology and trophic state. Observations and model results showed that deeper and clearer lakes had more ice-free winters, later ice cover freezing and earlier ice cover thawing dates, resulting in shorter ice-covered periods and fewer ice-covered days than shallow and less clear lakes. The 1947–2007 model hindcasts were implemented using FLake for eight Berlin-Brandenburg lakes without ice phenology observations. Results demonstrated past trends of later ice start and earlier ice end, shorter ice cover duration and an increase in ice-free winters. 相似文献
10.
A. L. Perchuk M. Burchard H.-P. Schertl W. V. Maresch T. V. Gerya H.-J. Bernhardt O. Vidal 《Contributions to Mineralogy and Petrology》2009,157(5):573-592
We demonstrate the possibility of studying several diffusion couples in a single run, i.e. under almost similar P–T–t– conditions, allowing direct comparison of the diffusion rates in different diffusion couples. Thus the duration of experimental
study and the risk of failure of expensive experimental equipment can be decreased considerably. The diffusion experiments
were carried out in piston-cylinder apparatus. Gem-quality garnets of almandine, spessartine and grossular compositions together
with inclusion-rich eclogitic garnets were embedded in a powder of natural pyrope and annealed together under dry conditions
at P = 1.9–3.2 GPa and T = 1,070–1,400°C. Diffusion profiles were measured by electron microprobe and fitted numerically on the basis of multicomponent
diffusion theory. The datasets derived from different diffusion couples yields parameters of the Arrhenius equation for Ca,
Mg and Fe in natural eclogitic garnets and Mg, Mn and Fe in gem-quality garnets. We have also studied the effect of grain-boundary
diffusion in the sintered pyrope matrix on interdiffusion on the basis of 2D modeling. Under conditions analogous to those
of our experimental runs, we show that observed irregularities in some measured diffusion profiles (not applied for the diffusion
modeling) can be directly related to the superposition of local grain-boundary diffusion on dominant volume diffusion. 相似文献