对虾一种无包涵体杆状病毒病原的PCR检测   总被引：4，自引：0，他引：4  

石拓  孔杰  刘萍  包振民  韩玲玲  徐怀恕 《海洋学报》2000,22(4):96-100

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.  相似文献   

黄河内蒙古段上覆水、悬浮物和底泥重金属特征及生态风险研究   总被引：24，自引：12，他引：12  

赵锁志  刘丽萍  王喜宽  李世宝  朱锁  郑萍  张青  张宝  蒙奎文 《现代地质》2008,22(2):304-312

系统地采集黄河内蒙古段上覆水、悬浮物和底泥样品,分析其中重金属元素的含量。采用地质累积指数法和潜在生态危害系数法,研究了黄河干流内蒙古段的上覆水、悬浮物和底泥的重金属沿程分布特征、污染程度和生态风险。分析结果显示:除了As元素,悬浮物中重金属元素含量总体上高于底泥中的含量。地质累积指数法评价结果表明:悬浮物和底泥属于无污染—中度污染,悬浮物总体上比底泥污染严重;潜在生态危害系数法评价结果表明:底泥和悬浮物中重金属的生态危害总体上属轻微状态;因此,生态风险指数法和地质累积指数评价结果基本一致。  相似文献   

高地震区公路隧道地震动力响应分析   总被引：2，自引：0，他引：2  

朱长安  高波  索然绪 《岩土工程技术》2008,22(5):232-235

基于土一结构相互作用理论,对高地震区一实际重大工程的公路隧道洞口段结构进行了抗震计算,得到了衬砌结构各控制点的位移、加速度及内力响应规律。结果表明：在人工合成地震波条件下,衬砌墙脚、拱腰为抗震薄弱位置;结构的加速度波形与输入波形相似;这些结果为抗震设计提供了一些依据。  相似文献   

祁连山北缘—河西走廊西段晚新生代逆冲推覆断裂发育模式   总被引：1，自引：0，他引：1  

陈柏林  王春宇  崔玲玲  刘建民 《地学前缘》2008,15(6)

祁连山北缘—河西走廊西段地处青藏高原东北缘,是我国新构造活动最强烈的地区之一。通过野外对研究区主要活动断裂的调查研究、结合年代学测试和综合分析,认为祁连山北缘—河西走廊西段地区的旱峡—大黄沟断裂、玉门断裂、新民堡断裂和阴洼山断裂都是晚更新世至全新世活动断裂。旱峡—大黄沟断裂形成于中新世晚期,一直活动至今;玉门断裂形成于上新世早期,在距今7.6ka左右和3.2ka左右发生过2次强烈的新构造活动(古地震),也是2002年玉门地震的发震断裂;阴洼山断裂形成于上新世晚期(3.0Ma左右),在距今19.9～21.0ka、8.6～10.0ka、4.1～5.0ka发生过3次强烈的新构造活动,也可能是1785年惠民堡(现新民堡)地震的发震断裂;但是位于近盆地中心的新民堡断裂形成时代明显晚的多,起始于中更新世晚期(0.17Ma左右),在距今28.3ka、4.4ka和1.2ka左右发生过强烈的3次新构造活动。在运动形式上,上述4条断裂均为自南南西向北北东的逆冲推覆。在变形性质上,新民堡断裂以非地震性的蠕滑为主,其他3条断裂主要为地震性的粘滑变形。在祁连山北缘—河西走廊西段的北北东剖面上,逆冲推覆断裂表现为前展-后展复合发育模式,即形成时代最新的是位于酒西盆地中心的新民堡断裂,在酒西盆地中心以南部分的剖面中断裂发育为前展式,由祁连山腹地向河西走廊盆地中心断裂形成时代越来越新,而酒西盆地中心以北部分的剖面中断裂发育为后展式,即从盆地中心到盆地北缘断裂形成时代越来越老。  相似文献   

上海“8.5”特大暴雨的成因和特点   总被引：5，自引：3，他引：5  

邵玲玲  黄炎 《气象》2002,28(8):47-50

利用WSR－88D多普勒雷达资料，通过分析2001年8月5日到8月6日产生于上海地区的特大暴雨过程的雷达回波特征，研究探讨这次过程的降水成因和降水特点。发现副热带高压边缘偏南气流的风速辐合、螺旋雨带中强回波短带的合并加强、风的垂直切变产生的辐合以及热带系统的高效率降水作用是这次暴雨过程的降水成因和降水特点。并提出了预报这类暴雨系统需要注意的要点：强降水回波的发生和发展加强往往和风场中的局地强辐合区相联系，利用多普勒雷达连续演变的风场资料，可及时了解辐合区的形成和演变过程，提前作出强降水可能性的预测。  相似文献   

流体短期前兆典型特征分析     

刘耀炜  曹玲玲  平建军 《国际地震动态》2004,(Z1):90

长期的地震实践表明,地下流体与地震活动有密切的关系,特别是在地震短期阶段,地震前兆中流体异常表现的最为明显,并且数量最多.由于在地震孕育过程中,能够引起浅层流体异常的动力源机制比较复杂,异常变化形态也复杂多样,这对于有效分析异常产生的机理造成了很大困难.本项研究工作,通过中国大陆和华北、西北、西南等分区研究,认为可以将这些复杂的异常现象归纳为较易识别的转折型(复合型)和单一型两类异常类型,并提出了这两类前兆异常类型的综合特点.  相似文献   

Late-Stage Ductile Deformation in Xiongdian-Suhe HP Metamorphic Unit, North-Western Dabie Shan, Central China     

SuoShutian ZhongZengqiu ZhouHanwen YouZhendong 《中国地质大学学报(英文版)》2004,15(3):262-274

New structural and petrological data unveil a very complicated ductile deformation history of the Xiongdian-Suhe HP metamorphic unit, north-western Dabie Shun, central China. The finegrained symplectic amphibolite-facies assemblage and coronal structure enveloping eclogite-facies garnet,omphacite and phengite etc., representing strain-free decompression and retrogressive metamorphism,are considered as the main criteria to distinguish between the early-stage deformation under HP metamorphic conditions related to the continental deep subduction and collision, and the late-stage deformation under amphibolite to greenschist-facies conditions occurred in the post-eclogite exhumation processes.Two late-stages of widely developed, sequential ductile deformations D3 and D4, are recognized on the basis of penetrative fabrics and mineral aggregates in the Xiongdian-Suhe HP metamorphic unit, which shows clear, regionally, consistent overprinting relationships. D3 fabrics are best preserved in the Suhe tract of low post-D3 deformation intensity and characterized by steeply dipping layered mylonitic amphibolites associated with doubly vergent folds. They are attributed to a phase of tectonism linked to the initial exhumation of the HP rocks and involved crustal shortening with the development of upright structures and the widespread emplacement of garnet-bearing granites and felsic dikes. D4 structures are attributed to the main episode of ductile extension (D^24) with a gently dipping foliation to the north and common intrafolial, recumbent folds in the Xiongdian tract, followed by normal sense top-to-the northductile shearing (D^24) along an important tectonic boundary, the so-called Majiawa-Hexiwan fault (MHF), the westward continuation of the Balifan-Mozitan-Xiaotian fault (BMXF) of the northern Dabie Shan. It is indicated that the two stages of ductile deformation observed in the Xiongdian-Suhe HP metamorphic unit, reflecting the post-eclogite compressional or extrusion wedge formation, the subhorizontal ductile extension and crustal thinning as well as the top-to-the north shearing along the high-angle ductile shear zones responsible for exhumation of the HP unit as a coherent slab, are consistent with those recognized in the Dabie-Sulu UHP and HP metamorphic belts, suggesting that they were closely associated in time and space. The Xiongdian-Suhe HP metamorphic unit thus forms part of the Triassic(250-230 Ma) collision orogenic belt, and can not connect with the South Altun-North Qaidam-North Qinline UHP metamorphic belt formed durin~ the Early Paleozoic (500-400Ma).  相似文献   

Establishment of apparent quantum yield and maximum ecosystem assimilation on Tibetan Plateau alpine meadow ecosystem     

XU Lingling  ZHANG Xianzhou  SHI Peili  YU Guirui 《中国科学D辑(英文版)》2005,48(Z1)

The alpine meadow is widely distributed on the Tibetan Plateau with an area of about 1.2×106kn2. Damxung County, located in the hinterland of the Tibetan Plateau, is the place covered with this typical vegetation. An open-path eddy covariance system was set up in Damxung rangeland station to measure the carbon flux of alpine meadow from July to October,2003. The continuous carbon flux data were used to analyze the relationship between net ecosystem carbon dioxide exchange (NEE) and photosynthetically active radiation (PAR), as well as the seasonal patterns of apparent quantum yield (α) and maximum ecosystem assimilation (Pmax).Results showed that the daytime NEE fitted fairly well with the PAR in a rectangular hyperbola function, with α declining in the order of peak growth period (0.0244 μmolCO2 · μmol-1pAR) ＞early growth period ＞ seed maturing period ＞ withering period (0.0098 μmolCO2 · μmol-1pAR).The Pmax did not change greatly during the first three periods, with an average of 0.433mgCO2· m-2· s-1, i.e. 9.829 μmolCO2· m-2· s-1. However, during the withering period, Pmax was only 0.35 mgCO2 · m-2 · s-1, i.e. 7.945 μmolCO2 · m-2 · s-1. Compared with other grassland ecosystems, the α of the Tibetan Plateau alpine meadow ecosystem was much lower.  相似文献   

Chemical weathering in the Upper Huang He (Yellow River) draining the eastern Qinghai-Tibet Plateau   总被引：8，自引：0，他引：8  

Lingling Wu  Jianhua Qin  Suzan van Der Lee 《Geochimica et cosmochimica acta》2005,69(22):5279-5294

We examined the fluvial geochemistry of the Huang He (Yellow River) in its headwaters to determine natural chemical weathering rates on the northeastern Qinghai-Tibet Plateau, where anthropogenic impact is considered small. Qualitative treatment of the major element composition demonstrates the dominance of carbonate and evaporite dissolution. Most samples are supersaturated with respect to calcite, dolomite, and atmospheric CO₂ with moderate (0.710-0.715) ⁸⁷Sr/⁸⁶Sr ratios, while six out of 21 total samples have especially high concentrations of Na, Ca, Mg, Cl, and SO₄ from weathering of evaporites. We used inversion model calculations to apportion the total dissolved cations to rain-, evaporite-, carbonate-, and silicate-origin. The samples are either carbonate- or evaporite-dominated, but the relative contributions of the four sources vary widely among samples. Net CO₂ consumption rates by silicate weathering (6-120 × 10³ mol/km²/yr) are low and have a relative uncertainty of ∼40%. We extended the inversion model calculation to literature data for rivers draining orogenic zones worldwide. The Ganges-Brahmaputra draining the Himalayan front has higher CO₂ consumption rates (110-570 × 10³ mol/km²/yr) and more radiogenic ⁸⁷Sr/⁸⁶Sr (0.715-1.24) than the Upper Huang He, but the rivers at higher latitudes are similar to or lower than the Upper Huang He in CO₂ uptake by silicate weathering. In these orogenic zones, silicate weathering rates are only weakly coupled with temperature and become independent of runoff above ∼800 mm/yr.  相似文献   

面向应用的航天遥感科学论证研究     

顾行发  余涛  高军  田国良  王春梅  郑逢杰  李娟  董文  米晓飞  胡新礼  谢勇  孟庆岩  刘其悦  杨健  卫征  张周威  方莉  林英豪  高海亮  赵利民  郑利娟  刘苗  李玲玲  孙源  程洋  张雅洲  黄祥志  臧文乾  徐辉  吴俣  柳鹏  刘东晖  邓安健 《遥感学报》2016,20(5):807-826

为适应中国民用航天遥感从科学试验向业务服务模式转变,更好地探索、了解与解决应用需求与航天遥感系统对接等方面遇到的技术问题,促进航天遥感统筹协调可持续发展,中国适时于2004年成立了国家航天局航天遥感论证中心。10余年来,论证中心以航天遥感系统为研究对象,系统开展了面向应用的航天遥感科学论证概念、理论方法、技术工程与应用研究。本文是论证中心团队长期从事航天遥感科学论证研究与实践的系统总结,介绍了遥感论证初步认知、遥感论证关注问题、遥感论证理论体系与模型方法集、遥感论证能力建设及遥感论证实践等方面内容,给出了遥感论证定义并详细分析了研究范围和内容,提出了由知识维、进程维和逻辑维所组成的遥感论证作用域3维空间结构,指出社会发展加快和信息化水平提高,带动整个航天遥感数据信息链向更大规模、更短响应时间周期、更综合数据集成、更高数据质量、更加智能化方向发展,航天遥感系统将进入新的"智慧遥感"发展阶段。得益于十余年来中国民用航天快速发展,我们经历了风云三号新型载荷校飞、多角度多光谱偏振遥感器论证、环境星应用工程论证等实践,取得了多方面理论方法的突破,并应用到2030民用航天发展规划、高分辨率对地观测系统、国家自然灾害空间信息基础设施、国家民用空间基础设施中长期发展、2030中国综合地球观测系统规划等论证当中。经过不断实践,快速迭代,形成了遥感论证理论体系及相应的十大模型方法,包括遥感信息流模型、遥感信息特征模型、遥感信息应用模型、遥感信息量分析模型、遥感数据工程模型、航天遥感系统结构模型、航天遥感系统状态描述模型、航天遥感系统质量模型、航天遥感系统发展动力模型及能力体系模型。这些模型方法全面反映了航天遥感系统特征、结构、状态、发展动力、条件等,可广泛用于对航天遥感系统进行顶层设计、规划、考察、分析、评价、预测,并开展实践探索。  相似文献