MicroRNAs identification and bioinformatics analysis in large yellow croaker Larimichthys crocea using an integrated comparative and ab initio approach     

房路京  肖世俊  韩兆方  王志勇 《中国海洋湖沼学报》2018,36(5):1707-1719

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基于卫星遥感数据的森林病虫害监测与评价     

王涵  赵文武  刘月  刘焱序 《地理研究》2019,74(4):831-843

森林病虫害由于在森林资源中造成的重大破坏而被人们称为“不冒烟的火灾”,其对生态系统的研究具有重要意义。现有基于遥感数据的病虫害研究多集中在森林病虫害的监测、爆发原因以及发病区域内生产力的变化情况,而对于森林病虫害发生后森林中植被指数与叶面积指数之间的相关性的变化情况还相对较少,处于需要持续性深入探讨的阶段。以加拿大不列颠哥伦比亚地区2002—2012年森林病虫害数据为基础,分析不同严重程度的病虫害对叶面积指数（LAI）与归一化植被指数（NDVI）和增强型植被指数（EVI）的影响。结果表明：① 受病虫害感染的像元在轻度（Light）、中度（Moderate）和重度（Severe）三个严重级别中,NDVI与LAI之间的相关性由弱变强,又由强变弱;② EVI与LAI之间的相关性,在轻度（Light）、中度（Moderate）和重度（Severe）三个严重级别的像元中则依次变强。这一研究将为今后利用遥感数据识别病虫害、评价生态系统影响提供基础。  相似文献   

Implementation of a coupled plastic damage distinct lattice spring model for dynamic crack propagation in geomaterials          下载免费PDF全文

Chao Jiang  Gao‐Feng Zhao 《国际地质力学数值与分析法杂志》2018,42(4):674-693

This paper studies dynamic crack propagation by employing the distinct lattice spring model (DLSM) and 3‐dimensional (3D) printing technique. A damage‐plasticity model was developed and implemented in a 2D DLSM. Applicability of the damage‐plasticity DLSM was verified against analytical elastic solutions and experimental results for crack propagation. As a physical analogy, dynamic fracturing tests were conducted on 3D printed specimens using the split Hopkinson pressure bar. The dynamic stress intensity factors were recorded, and crack paths were captured by a high‐speed camera. A parametric study was conducted to find the influences of the parameters on cracking behaviors, including initial and peak fracture toughness, crack speed, and crack patterns. Finally, selection of parameters for the damage‐plasticity model was determined through the comparison of numerical predictions and the experimentally observed cracking features.  相似文献   

Assessment of alternative adsorption models and global sensitivity analysis to characterize hexavalent chromium loss from soil to surface runoff          下载免费PDF全文

Chuan‐An Xia  Juxiu Tong  Bill X. Hu  Xiujie Wu  Alberto Guadagnini 《水文研究》2018,32(20):3140-3157

We investigate our ability to assess transfer of hexavalent chromium, Cr(VI), from the soil to surface runoff by considering the effect of coupling diverse adsorption models with a two‐layer solute transfer model. Our analyses are grounded on a set of two experiments associated with soils characterized by diverse particle size distributions. Our study is motivated by the observation that Cr(VI) is receiving much attention for the assessment of environmental risks due to its high solubility, mobility, and toxicological significance. Adsorption of Cr(VI) is considered to be at equilibrium in the mixing layer under our experimental conditions. Four adsorption models, that is, the Langmuir, Freundlich, Temkin, and linear models, constitute our set of alternative (competing) mathematical formulations. Experimental results reveal that the soil samples characterized by the finest grain sizes are associated with the highest release of Cr(VI) to runoff. We compare the relative abilities of the four models to interpret experimental results through maximum likelihood model calibration and four model identification criteria (i.e., the Akaike information criteria [AIC and AIC_C] and the Bayesian and Kashyap information criteria). Our study results enable us to rank the tested models on the basis of a set of posterior weights assigned to each of them. A classical variance‐based global sensitivity analysis is then performed to assess the relative importance of the uncertain parameters associated with each of the models considered, within subregions of the parameter space. In this context, the modelling strategy resulting from coupling the Langmuir isotherm with a two‐layer solute transfer model is then evaluated as the most skilful for the overall interpretation of both sets of experiments. Our results document that (a) the depth of the mixing layer is the most influential factor for all models tested, with the exception of the Freundlich isotherm, and (b) the total sensitivity of the adsorption parameters varies in time, with a trend to increase as time progresses for all of the models. These results suggest that adsorption has a significant effect on the uncertainty associated with the release of Cr(VI) from the soil to the surface runoff component.  相似文献   

Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris     

Zhansheng Guo  Leng Han 《新西兰海洋与淡水研究杂志》2018,52(2):232-246

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.  相似文献   

Area usage estimation and spatiotemporal variability in distribution patterns of southern right whales,Eubalaena australis,of southern Brazil          下载免费PDF全文

Eduardo Pires Renault‐Braga  Karina Rejane Groch  Paulo A. de Carvalho Flores  Eduardo R. Secchi  Luciano Dalla‐Rosa 《Marine Ecology》2018,39(3)

Southern right whales—Eubalaena australis (Desmoulins, 1822)—migrate seasonally from high‐latitude feeding grounds to coastal breeding and calving grounds at lower latitudes such as the southern coast of Brazil. Understanding how these whales are distributed along the coast is important for monitoring their postwhaling recovery and defining management strategies. In this study, we applied Kernel density estimators to aerial survey data to determine main occurrence and concentration areas of right whales in southern Brazil and investigate inter‐ and intra‐annual distribution patterns between 2003 and 2012. Our results show considerable variation in area usage within and among years, and changes in the general distribution pattern of right whales in the last years of the study. Intra‐annually, higher concentration area tended to expand from July to September and decrease in November. Some areas stood out as high‐density areas for right whales: Ribanceira/Ibiraquera, Itapirubá Sul/Sol, and from Arroio to Gaivota. Some evidences also suggest preferential areas for mother–calf pairs. The higher concentration area of right whales in southern Brazil was estimated at 52,541 km² and the occurrence area was 682.69 km², which is the whole study area. As right whale distribution in the region is likely expanding due to this population's current recovery, our study provides essential information for management plan of the Right Whale Environmental Protection Area.  相似文献   

秦皇岛近岸海域绿藻微观繁殖体的分布以及在绿潮形成中的作用   总被引：2，自引：0，他引：2  

韩红宾  宋伟  王宗灵  丁德文  袁超  张学雷  李艳 《海洋学报(英文版)》2019,38(8):72-77

2015年以来，秦皇岛近岸海域暴发了绿潮，对北戴河旅游区的环境和生态系统造成了严重影响。绿藻微观繁殖体在绿潮的形成过程中起到重要作用，主要包括孢子、配子、幼苗和营养片段。绿藻微观繁殖体作为绿潮的“种源”，其分布规律可以反映绿潮的“藻源”位置。本研究于2016年4-9月和2017年1月对秦皇岛近岸海域绿藻微观繁殖体的调查，探究了其分布规律以及生物量变化。结果显示，绿藻微观繁殖体主要分布在近岸海域，由近岸向远岸海域逐渐降低。绿藻微观繁殖体的数量在7、8月份最高，在冬季最低。受绿潮影响严重的海域微观繁殖体数量高于其它海域。秦皇岛近岸海域的绿藻微观繁殖体为该海域绿潮的种源，其分布规律表明秦皇岛近岸海域绿潮起源于本地。  相似文献   

两株南极枝孢霉属真菌Cladosporium sp.NJF4和NJF6的次级代谢产物研究          下载免费PDF全文

常俊男  田晓清  樊成奇  黄金昌  陆亚男  韩清华 《极地研究》2020,32(1):60-67

南极因其独特的自然环境成为潜在、重要的微生物资源库,是产生新型生物活性物质和先导化合物菌株的潜在种源地,南极微生物正在成为创新药物研究新的重要资源。虽然近年来对南极微生物次级代谢产物的研究逐渐增加,但与温带和热带微生物研究相比仍处于初级阶段。对从南极普里兹湾海洋沉积物中获得的两株枝孢霉属真菌Cladosporium sp. NJF4和NJF6进行次级代谢产物分离及结构鉴定,获得20个化合物。化合物结构类型包括甾醇(1)、倍半萜类(7—8)、生物碱类(9—14)、二酮哌嗪(2—5、15—17)、芳香酸(6、18—19)等,其中倍半萜类(7—8)为首次从枝孢霉属真菌中分离得到,以上研究将为丰富南极微生物次级代谢产物库奠定一定的研究基础。  相似文献   

Influence of Stress on Void Ratios of Compacted Crushed Rock Masses in Coal Mine Gobs     

Hu  Shengyong  Han  Dandan  Feng  Guorui  Zhang  Ao  Hao  Guocai  Hu  Lanqing  Zhu  Liping  Li  Bo 《Natural Resources Research》2020,29(2):1361-1373

The transfer and evolution of stress among rock blocks directly change the void ratios of crushed rock masses and affect the flow of methane in coal mine gobs. In this study, a Lagrange framework and a discrete element method, along with the soft-sphere model and EDEM numerical software, were used. The compaction processes of rock blocks with diameters of 0.6, 0.8, and 1.0 m were simulated with the degrees of compression set at 0%, 5%, 10%, 15%, 20%, and 25%. This study examines the influence of stress on void ratios of compacted crushed rock masses in coal mine gobs. The results showed that stress was mainly transmitted downward through strong force chains. As the degree of compression increased, the strong force chains extended downward, which resulted in the stress at the upper rock mass to become significantly higher than that at the lower rock mass. It was determined that under different degrees of compression, the rock mass of coal mine gobs could be divided, from the bottom to the top, into a lower insufficient compression zone (ICZ) and an upper sufficient compression zone (SCZ). From bottom to top, the void ratios in the ICZ sharply decreased and those in the SCZ slowly decreased. Void ratios in the ICZ were 1.2–1.7 times higher than those in the SCZ.

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Small hypervelocity particles captured in aerogel collectors: Location,extraction, handling and storage     

Andrew J. Westphal  Christopher Snead  Janet Borg  Eric Quirico  Pierre‐Ivan Raynal  Michael E. Zolensky  Gianluca Ferrini  Luigi Colangeli  Pasquale Palumbo 《Meteoritics & planetary science》2002,37(6):855-865

Abstract— It has now been about a decade since the first demonstrations that hypervelocity particles could be captured, partially intact, in aerogel collectors. But the initial promise of a bonanza of partially‐intact extraterrestrial particles, collected in space, has yet to materialize. One of the difficulties that investigators have encountered is that the location, extraction, handling and analysis of very small (10 μm and less) grains, which constitute the vast majority of the captured particles, is challenging and burdensome. Furthermore, current extraction techniques tend to be destructive over large areas of the collectors. Here we describe our efforts to alleviate some of these difficulties. We have learned how to rapidly and efficiently locate captured particles in aerogel collectors, using an automated microscopic scanning system originally developed for experimental nuclear astrophysics. We have learned how to precisely excavate small access tunnels and trenches using an automated micromanipulator and glass microneedles as tools. These excavations are only destructive to the collector in a very small area—this feature may be particularly important for excavations in the precious Stardust collectors. Using actuatable silicon microtweezers, we have learned how to extract and store “naked” particles—essentially free of aerogel—as small as 3 μm in size. We have also developed a technique for extracting particles, along with their terminal tracks, still embedded in small cubical aerogel blocks. We have developed a novel method for storing very small particles in etched nuclear tracks. We have applied these techniques to the extraction and storage of grains captured in aerogel collectors (Particle Impact Experiment, Orbital Debris Collector Experiment, Comet‐99) in low Earth orbit.  相似文献