Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect     

Chia  Shir Reen  Chew  Kit Wayne  Show  Pau Loke  Sivakumar  Manickam  Ling  Tau Chuan  Tao  Yang 《中国海洋湖沼学报》2019,37(3):898-908

Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.  相似文献   

Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil   总被引：1，自引：0，他引：1  

Mahalingam Brinda Lakshmi  Venkataramagounder Perumal Anandaraj  Manickam Velan 《洁净——土壤、空气、水》2013,41(1):86-93

The effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil–slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50–200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X‐100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X‐100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X‐100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites.  相似文献   

On the source of quasi-periodic oscillations of the black hole candidate GRS 1915+105: some new observations and their interpretation     

Anuj Nandi  Sivakumar G. Manickam  A. R. Rao  Sandip K. Chakrabarti 《Monthly notices of the Royal Astronomical Society》2001,324(1):267-272

A few classes of the light curve of the black hole candidate GRS 1915+105 have been analysed in detail. We discover that unlike the previous findings, quasi-periodic oscillations (QPOs) occasionally occur even in the so-called 'On' or softer states. Such findings may require a revision of the accretion/wind scenario of the black hole candidates. We conjecture that considerable winds that are produced in 'Off' states cool down as a result of Comptonization and fall back to the disc, creating an excess accretion rate and producing the so-called 'On' state. After the drainage of the excess matter, the disc goes back to the 'Off' state. Our findings strengthen the shock oscillation model for QPOs.  相似文献   

Optimization of Minimal Salt Medium for Efficient Phenanthrene Biodegradation by Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil Using Factorial Design Experiments     

Mahalingam Brinda Lakshmi  Karuppan Muthukumar  Manickam Velan 《洁净——土壤、空气、水》2013,41(1):51-59

This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed.  相似文献   

Removal of Chromium from Synthetic Effluent using Nymphaea rubra     

Rafi Yasmin Taj  Sahaderan Renganathan  Nagarajan Partha  Gurunathan Baskar  Manikom Velan 《洁净——土壤、空气、水》2009,37(10):787-792

In the present work, biosorption of Cr(VI) by Nymphaea rubra was investigated in batch studies. Batch experiments were conducted to study the effect of initial sorbent dosage, solution pH and initial Cr(VI) concentration. The results showed that the equilibrium uptake capacity was increased with decrease in biomass dosage. The Cr(VI) removal was influenced by the initial chromium compound concentration. Langmuir and Freundlich adsorption isotherm models were used to represent the equilibrium data. The Freundlich isotherm model was fitted very well with the equilibrium data when compared to Langmuir isotherm model. The sorption results were analyzed for pseudo‐first order and pseudo‐second order kinetic model. It was observed that the kinetic data fitted very well with the pseudo‐second order rate equation when compared to the pseudo‐first order rate equation. Fourier transform infrared spectrum showed the presence of different functional groups in the biomass. The surface morphology of the sorbent was exemplified by SEM analysis. Aquatic weeds seem to be a promising biosorbent for the removal of chromium ions from water environment. This paper reports the research findings of a laboratory‐based study on the removal of Cr(VI) from the synthetic solution using the dried stem of N. rubra as a biosorbent.  相似文献   

Equilibrium and Kinetic Studies on the Removal of Reactive Red 2 Dye from an Aqueous Solution Using a Positively Charged Functional Group of the Nymphaea rubra Biosorbent     

Sahadevan Renganathan  Jayabalan Kalpana  Mahendradas Dharmendira Kumar  Manickam Velan 《洁净——土壤、空气、水》2009,37(11):901-907

Nymphaea rubra stem was used as a low cost and easily available biosorbent for the removal of Reactive Red 2 dye from an aqueous solution. Initially, the effects of biosorbent dosage (0.2–1.0 g L^–1), pH (1–6), and dye concentration (30–110 mg L^–1) on dye removal were studied. Batch experiments were carried out for biosorption kinetics and isotherm studies. The results showed that dye uptake capacity was found to increase with a decrease in biosorbent dosage. Equilibrium uptake capacity was found to be greatest at a pH value of 2.0, when compared to all other pH values studied. The equilibrium biosorption isotherms were analyzed by the Freundlich and Langmuir models. The equilibrium data was found to fit very well with the Freundlich isotherm model when compared to the Langmuir isotherm model. The kinetic data was analyzed using pseudo-first order and pseudo-second order kinetic models. From the results, it was observed that the kinetic data was found to fit the pseudo-second order kinetic model very well. The surface morphology of the stem of the N. rubra biosorbent was exemplified by scanning electron microscopy. Fourier transform infrared analysis was employed to confirm the existence of an amine group in the stem of N. rubra.  相似文献   

Immobilization of Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil onto Papaya Stem (Carica papaya L.) and Its Application on Degradation of Phenanthrene     

Mahalingam Brinda Lakshmi  Karuppan Muthukumar  Manickam Velan 《洁净——土壤、空气、水》2012,40(8):870-877

This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 10⁶ cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study.  相似文献   

Electrochemical Degradation of Remazol Black B Dye Effluent     

Poppana Antony Soloman  Chiya Ahmed Basha  Manickam Velan  Veerappan Ramamurthi  Kandasamy Koteeswaran  Natesan Balasubramanian 《洁净——土壤、空气、水》2009,37(11):889-900

This study focused on the electrochemical degradation of hydrolyzed Remazol Black B (CI Reactive Black 5), a common diazo reactive dye, in aqueous solution. In the presence of various auxiliary dye chemicals, a typical Remazol Black simulated exhausted dyebath liquor was treated electrochemically in various basic electrochemical reactor configurations such as batch, batch recirculation and single pass systems. The effect of current density, supporting electrolyte concentration, electrolysis duration, specific electrode surface and fluid flow rate on pollutant removal and energy consumption performance of the systems was critically evaluated. Batch studies show the following operating parameters, current density: 2.5 A/dm², electrolysis duration: 6 h, and supporting electrolyte concentration: 3 g/L, were optimal for good overall performance of the system. Color removal was complete by 3 h of treatment for all combinations of parameters studied. The pollutant removal performance of the batch recirculation system was found to have improved considerably by increasing the flow rate. Performance of the batch recirculation system was comparatively better than the other rector configurations studied, with respect to capacity utilization and energy consumption.  相似文献   

Qualitative and quantitative assessment of TanDEM-X DEM over western Himalayan glaciated terrain     

Pratima Pandey  Surendar Manickam  Avik Bhattacharya  AL. Ramanathan  Gulab Singh  G. Venkataraman 《国际地球制图》2017,32(4):442-454

Glaciers have a high impact in the socio-economic sectors including water supply, energy production, flood and avalanches. A high precision digital elevation model (DEM) is required to monitor glaciers and to study various glacier processes. The present study deals with the qualitative and quantitative evaluation of the DEM generated from the bistatic TanDEM-X data by comparing it with GPS, Ice, Cloud, and land Elevation Satellite (ICESat) data and standard global DEMs such as Shuttle Radar Topography Mission (SRTM) and Advanced Space-borne Thermal Emission and Reflection Radiometer Global DEM (ASTER GDEM). The study area consists of highly undulating glaciated terrain in western Himalaya, India. The results reveal that TanDEM-X is slightly better than SRTM both qualitatively and quantitatively, whereas ASTER GDEM showing maximum discrepancy among the three DEMs. The Root Mean Square Error (RMSE) of the TanDEM-X DEM with respect to GPS is 3.5 m at lower relief and 11.9 m at glaciated terrain, against 6.7 and 12.5 m for SRTM and 9.3 and 19.8 m for ASTER GDEM, respectively, for the same sites. On an average, for the whole study area, the RMSE of TanDEM-X is 7.9 m, SRTM is 9.3 m and ASTER GDM is 14.2 m. The RMSE of TanDEM-X, SRTM and ASTER GDEM with respect to ICESat are 16.3, 19.9 and 101.1 m, respectively. It is evident from the analysis that though SRTM is closer to TanDEM-X in terms of accuracy in the mountainous terrain, however, TanDEM-X will be more useful for studying glacier dynamics and topography.  相似文献   

Naphthalene Degradation Kinetics of Micrococcus sp., Isolated from Activated Sludge     

Josephraj Jegan  Kuppusamy Vijayaraghavan  Ramalingham Senthilkumar  Manickam Velan 《洁净——土壤、空气、水》2010,38(9):837-842

Biodegradation of naphthalene by Micrococcus sp., isolated from the effluent of an activated sludge plant, was studied. The effects of pH (5–8), glucose concentration (100–1000 mg/L) and inoculum concentrations (1–5%) on the growth and naphthalene degradation potential of Micrococcus sp. were investigated. Maximum naphthalene degradation and subsequent high microbial growth were observed at optimum pH (pH 7), glucose concentration (500 mg/L) and inoculum concentration (3%). To investigate the maximum naphthalene tolerance potential of Micrococcus sp., very high concentrations of naphthalene (500–5000 mg/L) were used in the presence of non‐ionic surfactants. The examined surfactants (Triton X‐100 and Tween‐80) increased the bioavailability of naphthalene to the microbes and Complete naphthalene degradation by Micrococcus sp. was observed at an initial naphthalene concentration of 500 mg/L. However, the degradation potential decreases as the naphthalene concentration increases. Very high naphthalene concentrations also affected the growth of microbes and the corresponding substrate inhibition kinetics was described using four models (Haldane, Webb, Edward and Aiba). Based on correlation coefficient and percentage error values, all four substrate kinetic models were able to describe the dynamic behavior of naphthalene biodegradation by Micrococcus sp.  相似文献