罗非鱼鱼皮多肽对H_2O_2诱导的HaCaT细胞氧化应激损伤的保护作用     

梁鹏  刘雪梅  杨萍  周春霞  洪鹏志 《广东海洋大学学报》2018,(4)

【目的】分析罗非鱼鱼皮多肽(TSP)对H_2O_2诱导HaCaT细胞氧化损伤的保护作用及其作用机制。【方法】将HaCaT细胞随机分成空白组(细胞正常培养)、对照组(800μmol/L的H_2O_2作用24 h)和3个罗非鱼鱼皮多肽浓度组(10、20、50μmol/L TSP),应用MTT比色法检测细胞活力,DCFH-DA法检测细胞内活性氧(ROS)含量,蛋白质印迹法测超氧化物歧化酶(SOD)和谷氨酰转肽酶(GGT)含量。【结果】与空白组相比,对照组的细胞活力明显下降(P﹤0.05),细胞内ROS含量增强,GGT表达量增加,SOD表达量减少;与对照组相比,随着多肽组浓度增加,细胞活力增强,细胞内ROS含量减少,GGT表达量减少,SOD表达量增加。【结论】10~50μmol/L罗非鱼皮多肽TSP能够提高细胞活力,通过清除细胞内的ROS,抑制GGT的表达,增加SOD的表达,达到保护H_2O_2诱导的HaCaT细胞的氧化应激损伤的效果。  相似文献   

FAST VLBI: current status and future plans     

Ru-Rong Chen  Hai-Yan Zhang  Cheng-Jin Jin  Zhi-Shen Gao  Yan Zhu  Kai Zhu  Peng Jiang  You-Ling Yue  Ji-Guang Lu  Bo Zhang  Wu Jiang  Ren-Jie Zhu  Shao-Guang Guo  Bo Xia  Rong-Bing Zhao  FAST Collaboration 《天文和天体物理学研究(英文版)》2020,(5):165-170

The Five-hundred-meter Aperture Spherical radio Telescope(FAST)is the largest single-dish radio telescope in the world,and is now being commissioned after the first light in September 2016.Very long baseline interferometry(VLBI)is among the key science topics according to the original design.The FAST VLBI system has been established,and the first VLBI fringe has been successfully obtained.FAST will significantly improve the sensitivity of the existing VLBI networks in the future,and some science projects in need of high sensitivity will benefit from its participation.  相似文献   

Study on Application of Polyenzyme Method to Offal of Harengula zunasi     

邓尚贵  杨萍  夏杏洲 《中国海洋湖沼学报》2003,21(4):312-318

The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest.  相似文献   

Jurassic Tectonics of North China: A Synthetic View   总被引：21，自引：1，他引：20  

ZHANG Yueqiao  DONG Shuwen  ZHAO Yue  ZHANG Tian 《《地质学报》英文版》2008,82(2):310-326

This paper gives a synthetic view on the Jurassic tectonics of North China, with an attempt to propose a framework for the stepwise tectonic evolution history. Jurassic sedimentation, deformation and magmatism in North China have been divided into three stages. The earliest Jurassic is marked by a period of magmatism quiescence （in 205-190 Ma） and regional uplift, which are considered to be the continuation of the “Indosinian movement” characterized by continent-continent collision between the North and South China blocks. The Early to Middle Jurassic （in 190-170 Ma） was predominated by weak lithospheric extension expressed by mantle-derived plutonism and volcanism along the Yanshan belt and alongside the Tan-Lu fault zone, normal faulting and graben formation along the Yinshan- Yanshan tectonic belt, depression and resuming of coal-bearing sedimentation in vast regions of the North China block （NCB）. The Middle to Late Jurassic stage started at 165y.5 Ma and ended up before 136 Ma; it was dominated by intensive intraplate deformation resulting from multi-directional compressions. Two major deformation events have been identified. One is marked by stratigraphic unconformity beneath the thick Upper Jurassic molasic series in the foreland zones of the western Ordos thrust-fold belt and along the Yinshan-Yanshan belt; it was predated 160 Ma. The other one is indicated by stratigraphic unconformity at the base of the Lower Cretaceous and predated 135 Ma. During this last stage, two latitudinal tectonic belts, the Yinshan-Yanshan belt in the north and the Qinling-Dabie belt in the south, and the western margin of the Ordos basin were all activated by thrusting; the NCB itself was deformed by the NE to NNE-trending structural system involving thrusting, associated folding and sinistral strike-slip faulting, which were spatially partitioned. Foliated S-type granitic plutons aged 160-150 Ma were massively emplaced in the Jiao-Liao massif east of the Tan-Lu fault zone and indicate important crustal thicken  相似文献   

High resolution profiles of trace metals in pore waters of marine and riverine sediments assessed by DET and DGT     

Yue GAO Martine Leermakers Gabriel Billon Baghdad Ouddane Jean-claude Fisher Willy Baeyens 《中国地球化学学报》2006,25(B08):199-199

In polluted aquatic systems, toxic metals are often accumulated in bottom sediments. They are, however, not necessarily stored definitively because diagenetic processs can modify redox, pH and even the amount of complexing ligands, releasing the trace metals back into the pore waters and the water column. Especially the labile metal fraction in the pore waters is important since this is the bioavailable fraction determining the bio-toxicity of the sediments. The goal of our study was therefore to assess, with novel sampling techniques, this bioavailable metal fraction in the pore waters as well as the flux towards the overlying water column. High-resolution profiles of trace metals in pore waters of marine and riverine sediments were assessed by DET （diffusive equilibrium in thin films） and DGT （diffusive gradients in thin films） gel techniques. The DET technique uses a diffusive gel layer that equilibrates with the aquatic system and with this technique the concentrations of total dissolved trace metals are obtained directly. The DGT technique uses an acrylamide diffusive gel backed by a resin gel （Chelex） which binds trace metals. With the DGT technique only labile species of selected metals can be captured. According to the redox potential measurements, the marine sediments were suboxic （200 mV to -220 mV versus Ag/AgCl electrode）, while the riverine sediments were completely anoxic （-160 mV to -220 mV versus Ag/AgCl electrode）. This redox potential was apparently controlling the trace metals species in the pore waters： for example a strong correlation between Mn and Co was found in the riverine sediments （for DET and DGT sampling）, while in the marine sediments trace metals presented various behaviors.  相似文献   

淄博煤田共生腐泥煤与腐植煤化学组成对比     

刘桂建杨萍玥  彭子成王桂梁 《地质地球化学》2003,31(1):23-27

为正确区分腐泥煤与腐植煤的地球化学特征，对山东淄博煤田10个地质勘探钻孔中共生的腐温煤与腐植煤样品的水分、硫含量、发热量、灰产率及灰成分、微量元素等进行了分析测试和对比研究，研究表明与共生的腐植煤相比，大部分腐泥煤具有低硫、高灰、高密度、富含微量元素等特点。二者在地球化学特征上的明显差异是因为其成煤物质基础不同。  相似文献   

SEDIMENTATION STUDY ON PUMPED-STORAGE POWER STATION OF PUSHI RIVER     

WANG Tiefeng Northeast Investigation  Design  Research Institute  China ZHANG Zhi Northeast Investigation  Design  Research Institute  China YI Yue Northeast Investigation. Design  Research Institute  China 《国际泥沙研究》1997,(3)

I.INTRODUCTIONThePtlmped-storagepoll,erprojectofPushiriverisconsistedofupperreservoir,lobal-erreset'oir.watertransmissionSystemandpotvergeneratingsystem.ThelowerreservoirIviththecatchmentbasinareaof1141kmZisthewatersupplierl-c'hichislocatedonthedownstreamofthePushiriverinthesouthernpactofNortheastChina.Theupperreservoirisabout300-400metershigherthanthelowerresen!oirattheleftsideoftheriverwithcatchmentbasinareaof1.12km2.Thecharacteristicsoftheprojectandreservoirareshottviintablel-l.Ino…  相似文献   

地下连续墙质量检测成果分析          下载免费PDF全文

杨萍  刘康和 《探矿工程》2004,31(11):18-20

为保证地下连续墙的施工质量，发挥其应有的设计功能，对其质量的检测是十分必要的，而传统的钻孔、开挖、围井等方法，不但破坏墙体影响其功能的正常发挥，而且费时费力，局限性很大，利用地球物理探测技术进行地下连续墙的无损检测已成为工程质量检查的必然发展方向。详细说明了地下连续墙无损检测技术的原理、实测方法和数据处理过程及其应用效果，并就有关问题进行了探讨。  相似文献   

科尔沁沙地土地沙漠化的历史与现状   总被引：9，自引：6，他引：9  

任鸿昌  吕永龙  杨萍  陈惠中  史雅静 《中国沙漠》2004,24(5):544-547

科尔沁沙地是我国自然条件相对较好的一个沙地，有着广阔的治理前景。回顾科尔沁沙地的历史变化，认为科尔沁沙地是一个全球气候变化大趋势下由人类生产活动过度而形成的沙漠化区域，在金代至清代之间由于人为活动强度减弱，曾经发生明显的沙漠化逆转。根据全国沙漠化普查所获得的资料，研究分析了科尔沁沙地土地沙漠化现状及其特点，结合土地沙漠化发生的历史过程，讨论了这一地区沙漠化的治理策略。  相似文献   

海绵城市建设下的生态效应分析——以常德市穿紫河流域为例     

王夏青  孙思远  杨萍  邹小燕  易巧梅  彭保发  许建伟 《地理学报》2019,74(10):2123-2135

海绵城市建设被认为是解决城市内涝、水污染和水资源短缺、热岛效应等问题的有效途径之一。中国自2015年起共确立30个海绵城市建设试点城区,为今后海绵城市建设在全国范围的推广提供示范。基于实地调研、查阅资料并对比分析穿紫河流域海绵城市建设前后的水文、植被和气候等自然要素变化。结果表明：常德市城市内涝有效缓解,但在暴雨发生时局部仍出现积水和内涝现象;穿紫河水质总体上由劣Ⅴ类转变为Ⅲ类,但在冬季周期性恶化及污染物富集;穿紫河流域生物多样性显著增加,同时也表现出植物种类单一、具有环境净化功能的植物种类较少、生态系统不完整和管理不善等问题;常德市夏季的热岛效应有一定程度缓解。常德市2004-2019年的海绵城市建设显著提升了穿紫河流域的生态效应,为该地区的社会经济发展和人居环境改善提供了重要的生态基础,也为合理评价其他试点城区海绵城市建设效果提供借鉴。  相似文献