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1.
日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究 总被引：6，自引：1，他引：6
以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。 相似文献
2.
Takeshi Matsuno Seiichi Kanari Chikashi Kobayashi Toshiyuki Hibiya 《Journal of Oceanography》1994,50(4):437-448
The strength of the vertical mixing in the bottom mixed layer near the continental shelf break in the East China Sea was directly measured with the Micro-Scale Profiler (MSP). It has been shown that there is no significant statistical relation between the turbulent energy dissipation and the degree of the stratificationN
2. It seems that the vigorous turbulence occurs not constantly but intermittently in the bottom mixed layer so that a large variation of is found depending on the time. In contrast to , the coefficient of the vertical eddy diffusivityK
z is mostly determined byN such thatK
z is large in the bottom mixed layer and small in the thermocline. Large value ofK
z in the bottom mixed layer is also found in the time series ofK
z estimated in terms of Richardson number calculated from the data obtained with electromagnetic current meters. The value ofK
z more than 10 cm2s–1 frequently occur in the layer of 20–25 m thick just above the bottom. 相似文献
3.
Some numerical experiments by the barotropic nonlinear two dimensional models are performed to study the water circulations in Lake Biwa, especially to study the large anticlockwise gyre in the north basin. The wind fields used in the experiments have no rotational component. This gyre is induced by the southerly wind and is approximately on the geostrophic balance. The vorticity of this gyre is contributed from the vertical stretching of the vortex tube by the variable bottom topography and the inertia term. But the latter term does not become effective if the bottom profile of the basin is flat. Therefore the horizontal circulation in the barotropic closed basin is the “topographic gyres”. The bottom stress has a little contribution to the vorticity balance of the gyre. 相似文献
4.
The phase velocity and the attenuation coefficient of compressional seismic waves, propagating in poroelastic, fluid-saturated, laminated sediments, are computed analytically from first principles. The wavefield is found to be strongly affected by the medium heterogeneity. Impedance fluctuations lead to poroelastic scattering; variations of the layer compressibilities cause inter-layer flow (a 1-D macroscopic local flow). These effects result in significant attenuation and dispersion of the seismic wavefield, even in the surface seismic frequency range, 10–100 Hz. The various attenuation mechanisms are found to be approximately additive, dominated by inter-layer flow at very low frequencies. Elastic scattering is important over a broad frequency range from seismic to sonic frequencies. Biot's global flow (the relative displacement of solid frame and fluid) contributes mainly in the range of ultrasonic frequencies. From the seismic frequency range up to ultrasonic frequencies, attenuation due to heterogeneity is strongly enhanced compared to homogeneous Biot models. Simple analytical expressions for the P -wave phase velocity and attenuation coefficient are presented as functions of frequency and of statistical medium parameters (correlation lengths, variances). These results automatically include different asymptotic approximations, such as poroelastic Backus averaging in the quasi-static and the no-flow limits, geometrical optics, and intermediate frequency ranges. 相似文献
5.
The Pecos River, situated in eastern New Mexico and western Texas, receives water from a drainage area of 91 000 km2. There are primarily two major water inputs, namely snowmelt from winter storms in the headwater region of the southern Rocky Mountains and runoff from warm-season monsoonal rainfall in the lower valley. The Pecos River suffers from high levels of total dissolved solids (TDS >5000 mg L−1) under normal flow conditions. This not only poses serious problems for agricultural irrigation and safe drinking water supply, but also results in a permanent loss of biodiversity. This study examines changes in stream flow and water chemistry of the Pecos River over the last 70 a to better understand the long-term variability in stream salinity and the role of agricultural practices in salt transfer. A TDS record from the lower Pecos River near Langtry (Texas) back to 1935 was extracted to show a distinct pattern of decadal variability similar to the Pacific Decadal Oscillation (PDO), in which stream salinity is overall above average when the PDO is in positive (warm) phase and below average when the PDO is in negative (cold) phase. This is due to: (1) the dissolved salts contributed to the river are largely from dissolution of NaCl and CaSO4-bearing minerals (e.g., halite and gypsum) in the upper basin, (2) the amount of the dissolved salts that reach the lower basin is mainly determined by the stream flow yield in the upper basin and (3) the stream flow yield from the upper basin is positively correlated with the PDO index. This further attests that large-scale climatic oscillation is the major source of long-term changes in stream flow and salinity of the Pecos River. On the other hand, there is also a strong indication that the rate of salt export has been affected by reservoir operations and water diversions for agricultural practices. 相似文献
6.
Contaminations in sediments by polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs were investigated at 44 sites in Osaka Bay, Japan. Concentrations of total PAHs and alkylated PAHs were in the range 6.40–7800 ng/g dry weights and 13.7–1700 ng/g dry weights, respectively. The PAH concentrations tended to be higher along the shoreline in the vicinities of big ports, industrialized areas, and densely populated regions such as the cities of Osaka and Kobe. The major sources appeared to be pyrogenic or both pyrogenic and petrogenic at most of the sites. PAH concentrations were remarkably high at a site near Kobe, where the concentrations of dibenzo(a,h)anthracene and benzo(g,h,i)perylene exceeded the effects-range-medium concentration and eight PAHs were above the corresponding effects-range-low concentrations. Those PAHs may have been derived from the great fire associated with the large earthquake in 1995. 相似文献
7.
Jiro Koyama Chie Imakado Seiichi Uno Takako Kuroda Shouichi Hara Takahiro Majima Hideyuki Shirota Nathaniel C. Añasco 《Marine pollution bulletin》2014
To assess risks of chemically-dispersed oil to marine organisms, oil concentrations in the water were simulated using a hypothetical spill accident in Tokyo Bay. Simulated oil concentrations were then compared with the short-term no-observed effect concentration (NOEC), 0.01 mg/L, obtained through toxicity tests using marine diatoms, amphipod and fish. Area of oil concentrations higher than the NOEC were compared with respect to use and non-use of dispersant. Results of the simulation show relatively faster dispersion near the mouth of the bay compared to its inner sections which is basically related to its stronger water currents. Interestingly, in the inner bay, a large area of chemically-dispersed oil has concentrations higher than the NOEC. It seems emulsifying oil by dispersant increases oil concentrations, which could lead to higher toxicity to aquatic organisms. When stronger winds occur, however, the difference in toxic areas between use and non-use of dispersant is quite small. 相似文献
8.
Correlation of large displacement drained shear strength of landslide soils measured by direct shear and ring shear devices 总被引：2，自引：0，他引：2
Shriwantha Buddhi Vithana Shinya Nakamura Seiichi Gibo Anshun Yoshinaga Sho Kimura 《Landslides》2012,9(3):305-314
Based on a laboratory study of drained direct shear tests of remoulded, pre-cut and polished specimens and drained ring shear tests of uncut and remoulded specimens of slip surface materials of five landslide soils, significant correlations of the mobilized shear strength parameters, cohesion (c, c r) and internal friction angle (Φ, Φr), are proposed. The investigated soils consisted of the slip surface material belonging to tuffaceous clay, mudstone, loess and siltstone. Most of the previous studies on residual shear strength measured by reversal direct shear and ring shear devices have obtained significantly different results using the two devices, even when pre-cut and polished specimens were used in the direct shear device. In this study, the residual shear strength values of remoulded specimens measured by a ring shear device are shown to significantly correlate with the drained large displacement shear strength values of remoulded specimens, which were measured using pre-cut and polished specimens in a direct shear device. The correlation between the cohesion measured in the two shear devices is expressed by the linear relationship, $ {c_{\text{r}}} = 0.{7394}c - {6}.{6857} $ , while the correlation between the friction angle measured in the two devices is expressed by the linear relationship, $ {\Phi_{\text{r}}} = {1}.0{852}\Phi - {6}.0{247} $ . The proposed linear correlations for effective cohesion (c′) and effective friction angle (Φ′) have yielded significant coefficients of determination within an effective normal stress range of 30–150?kN/m2. 相似文献
9.
Uno S Tanaka H Miki S Kokushi E Ito K Yamamoto M Koyama J 《Marine pollution bulletin》2011,63(5-12):477-481
This paper reports for the first time the detection and occurrence of nitroarenes (NPAHs) in marine organisms. Mussels and oysters collected from Osaka Bay, Japan, had total NPAHs concentrations that ranged from 2380 to 24,688 pg/g dry and 2672 to 25,961 pg/g dry, respectively. Relatively higher concentrations were detected in sampling sites located near the central district and suburbs of Osaka City implying that the most probable sources of NPAHs in the two bivalves are exhaust gases and smokes emitted by automobiles and industrial plants. Bivalves had relatively higher residues of 1-nitronaphthalene, 2-nitronaphthalene, 3-nitrophenanthrene, and 9-nitrophenanthrenes. Residues of 2-nitrofluorene, 1-nitropyrene, 4-nitropyrenes, and 6-nitrochrysene were much lower compared to nitronaphthalenes and nitrophenanthrenes. Inter-species differences was only observed for 2-nitronaphthalene with oysters exhibiting significantly higher residues than mussels. 相似文献
10.
固定标本的DNA提取及PCR扩增 总被引：4，自引：1，他引：3
报道福尔马林、酒精固定的日本绒螯蟹标本的 DNA提取及 PCR扩增 ,并与冷冻、煮沸标本进行了比较。结果表明 ,福尔马林固定标本与酒精固定标本一样可以提取 DNA,加入的蛋白酶 K量越多 ,DNA的回收率越高。以提取的 DNA为模板 ,进行了线粒体 DNA12 S r RNA和 D- loop基因片段的 PCR扩增 ,经琼脂糖凝胶电泳 PCR扩增产物后均得到了与期待的碱基长度相一致的清晰谱带。 相似文献