Stable carbon-isotopic compositions of lipids isolated from the ammonia-oxidizing chemoautotroph Nitrosomonas europaea     

Susumu Sakata  John M. Hayes  Michel Rohmer  Alan B. Hooper  Myriam Seemann 《Organic Geochemistry》2008,39(12):1725

For the ammonia-oxidizing bacterium Nitrosomonas europaea, grown autotrophically using semicontinuous culturing, average biomass was depleted in ¹³C relative to CO₂ dissolved in the medium by ca. 20‰ and the total-lipid extract was depleted in ¹³C relative to biomass by 3.7‰. The n-alkyl lipids (weighted average of fatty acids) and isoprenoid lipids (weighted average of hopanoids) were both depleted in ¹³C relative to biomass by about 9‰. The large depletion in the isoprenoid lipids seems to indicate that isotopic fractionations associated with the biosynthesis of methylerythritol phosphate (MEP) affected at least two carbon positions in each isoprene unit. Among the fatty acids, trans-9-hexadecenoic acid was most depleted (13.0‰ relative to biomass), followed by cis-9- hexadecenoic acid (9.6‰) and hexadecanoic acid (6.9‰). Isotopic relationships between the three acids suggest that significant isotope effects were associated with the desaturation and cis to trans isomerization of fatty acids. Given these observations, hopanoids produced by ammonia-oxidizing bacteria growing in natural waters are likely to be depleted in ¹³C by 26–30‰ relative to dissolved CO₂. Since CO₂ at aquatic oxyclines is often depleted in ¹³C, the range of δ values expected for hopanoids is ca. −34‰ to −55‰. The δ values of geohopanoids observed in numerous studies and attributed to unspecified chemoautotrophs fall within this range.  相似文献   

Identification of constitutive androstane receptor cDNA in northern fur seal (Callorhinus ursinus)     

Hiroki Sakai  Hisato Iwata  Eun-Young Kim  Shinsuke Tanabe  Norihisa Baba 《Marine environmental research》2004,58(2-5):107

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Controlled experiment in the ionosphere with a rocket-borne plasma gun     

Nobuki Kawashima  Susumu Sasaki  Osamu Kaneko  Akira Yamori  Ryo Okamura  Shoichi Okamura 《Planetary and Space Science》1978,26(4):367-373

A controlled active experiment was performed by a plasma gun on-board a rocket to study the plasma stream across the magnetic field in space. A mother and daughter system was employed. An 8 kV 0.8 μF (25 J) capacitor bank on the daughter rocket was discharged every 12 s and about 10¹⁶ ion and electron pairs were ejected. A plasma signal was detected on the electron temperature probe. The propagation speed of the plasma stream is estimated to be 10⁶ cm/s, considerably lower than that obtained in the prelaunch test. The amplitude of the plasma signal decays as the inverse square of the mutual distance between the mother and daughter rockets and this decay is much slower than the free streaming. The importance of the geomagnetic field on the plasma propagation in space is discussed.  相似文献   

Polar and neutral isopranyl glycerol ether lipids as biomarkers of archaea in near-surface sediments from the Nankai Trough   总被引：1，自引：0，他引：1  

Masahiro Oba  Susumu Sakata  Urumu Tsunogai 《Organic Geochemistry》2006,37(12):1643

The molecular and carbon isotopic compositions of polar isopranyl glycerol ether lipids, which are direct indicators of viable archaea, and neutral isopranyl glycerol ether lipids, which are derived from polar lipids via hydrolysis, in near-surface sediments from a methane seep in the Nankai Trough (off central Japan) were investigated. Procedures for extracting, separating and derivatizing polar and neutral ether lipids for detection using gas chromatography were first examined with one sediment sample and a cultivated methanogen. For all sediment samples, archaeol and hydroxyarchaeol were detected in both the polar and neutral ether lipid fractions. Acyclic and cyclic biphytanes were also detected in both types of lipid fractions after treatment with HI/LiAlH₄ for ether cleavage and alkylation. The δ¹³C values of archaeol, sn-2-hydroxyarchaeol, and sn-3-hydroxyarchaeol in the sample from 0.82 m below the seafloor were lower than −100‰ relative to PDB, indicating that diverse living methanotrophic archaea are present in the seep sediments. Biphytanes released from polar ether lipids in the same sample were less depleted in δ¹³C (−71‰ to −36‰). The wide range of δ¹³C values suggests that the biphytanes were derived not only from methanotrophic but also from non-methanotrophic archaea, and that the relative contributions of the methanotrophic and non-methanotrophic archaea differed, depending on the biphytane compound. The vertical profiles and δ¹³C values of the neutral ether lipids were similar to those of the intact polar ether lipids, suggesting that neutral ether lipids derived from fossil archaea in the samples had mainly been lost by the time of sampling.  相似文献   

Non-destructive monitoring of trace element levels in short-tailed albatrosses (Phoebastria albatrus) and black-footed albatrosses (Phoebastria nigripes) from Torishima Island, Japan using eggs and blood     

Tokutaka Ikemoto  Takashi Kunito  Shinsuke Tanabe  Miyako Tsurumi  Fumio Sato  Nariko Oka 《Marine pollution bulletin》2005,51(8-12):889

Concentrations of 18 trace elements (V, Cr, Mn, Co, Cu, Zn, Se, Rb, Sr, Mo, Ag, Cd, Sb, Cs, Ba, Hg, Tl and Pb) were determined in abandoned eggs of short-tailed albatrosses and abandoned eggs and blood of black-footed albatrosses from Torishima Island, Japan in 2002. Mercury concentration was highest among the toxic elements in egg content of both the two species. In some egg contents of black-footed albatrosses, Hg concentrations were higher than the threshold level that may cause decreased hatchability and aberrant nesting behavior in some avian species. Concentrations of Cd in egg content and Ba in egg content and shell increased with an increase in egg breadth/length ratio, whereas Hg in egg content showed an opposite trend in black-footed albatrosses. Since egg breadth/length ratio is known to increase with the age of mother bird, this result may imply that concentrations of these elements in eggs vary with age of mother bird. In blood of black-footed albatross chicks, concentration of Hg was highest among the toxic elements and the Hg concentration showed an increase in the later growth stages. In contrast, concentration of Sr in blood decreased with growth stage. Since the behaviour of Sr is similar to that of Ca in animals, it is conceivable that Sr was used along with Ca for the formation of bone.  相似文献   

Computer-aided design of bus route maps     

Yukio Sadahiro  Takahito Tanabe  Maxime Pierre  Koichi Fujii 《制图学和地理信息科学》2016,43(4):361-376

The bus route map is a diagram that aims to convey necessary information for map readers to find an appropriate way of moving from an origin to a destination. Design of bus route map is a complicated and time-consuming task that requires careful consideration of readability and aesthetics. This paper proposes a new computational method for designing bus route maps. The method helps us to reduce six types of undesirable elements in bus route maps, i.e., gap, shift, crossing, overlap, misalignment, and acute bend. The method consists of two phases: line layout phase determines the relative order of bus routes on each road segment and map layout phase calculates the actual position of bus routes drawn on a map. This paper applies the method to the design of bus route maps of Chiba City, Japan. The result supports the effectiveness of the method as well as reveals open topics for future research.  相似文献   

Mechanism of enrichment of trace metals on fine sludges collected from filtration plants     

Hisayuki Teraoka  Susumu Nakashima 《Environmental Geology》1990,16(2):143-148

Fine sludges were collected from five filtration plants, and the partitioning of ten metals (Ag, Cd, Mn, Zn, Pb, Cu, Sn, Co, Ni, and Fe) in them was determined by selective leaching techniques. (1) The available amounts, which shows the total of each metal leached between 1 M CH₃COONH₄ and 30 percent H₂O₂, for Ag, Cd and Mn, ranged from 51 to 98 percent for five sludges. (2) The available amounts for Zn, Pb, Cu, and Sn were 47–92 percent for five sludges. (3) The most important fraction for Co, Ni, and Fe, except the Inagawa sludge, which is markedly polluted by organic matter, was the crystalline particle. Therefore, the above metals, except Co, Ni, and Fe, are thought to be enriched on ion-exchangeable sites, organic matter, hydrous Fe/Mn oxides, and sulfides in fine sludges.  相似文献   

Persistent organochlorine residues in sediments from the Chukchi Sea, Bering Sea and Gulf of Alaska   总被引：14，自引：0，他引：14  

Hisato Iwata  Shinsuke Tanabe  Mari Aramoto  Norio Sakai  Ryo Tatsukawa 《Marine pollution bulletin》1994,28(12):746-753

Three surface sediments and two sediment cores were collected from the Gulf of Alaska, Bering Sea and Chukchi Sea, and analysed for persistent organochlorines (OCs). The geographical distributions of OCs showed different patterns according to their physicochemical properties. The concentrations of HCHs and HCB revealed rather uniform distribution, suggesting their more transportable nature in long-range atmospheric transport. On the other hand, DDTs and PCBs were predicted to be less transportable via the atmosphere due to the decreasing trends of residue levels in sediments from south to north. The OC profiles in the sediment core from the Gulf of Alaska which seemed to be preserved without turbation revealed the elevated residue levels from bottom to surface layers. This implies that the aerial inputs of OCs in the cold ocean are still continuing significantly. The accumulation rates of OCs into sediments were rather smaller than the atmospheric inputs, indicating that the residue levels in water bodies are unlikely to decrease rapidly in the near future.  相似文献   

Symmetrical fft technique and its applications to earthquake engineering     

Hiroshi Katukura  Susumu Ohno  Masanori Izumi 《地震工程与结构动力学》1989,18(5):717-725

The objective of this paper is to propose an FFT technique symmetrical for time and frequency, in which the symmetrization is done by introducing generating functions. By the symmetrical treatment, it becomes possible to derive several complementary relations in the time and frequency domains. Based on the idea of the symmetrical FFT technique, the phase problem of seismic waves is discussed not only in the frequency domain but also in the time domain. Furthermore, the deconvolution technique to evaluate the minimum-phase-shift and all-pass functions of seismic waves, which is not discussed sufficiently in earthquake engineering, is developed in the two domains. Numerical examples to present these basic ideas are also illustrated in the symmetrical framework.  相似文献   

Non‐linear system identification of the versatile‐typed structures by a novel signal processing technique     

Jian Zhang  Tadanobu Sato  Susumu Iai 《地震工程与结构动力学》2007,36(7):909-925

Non‐linear structural identification problems have raised considerable research efforts since decades, in which the Bouc–Wen model is generally utilized to simulate non‐linear structural constitutive characteristic. Support vector regression (SVR), a promising data processing method, is studied for versatile‐typed structural identification. First, a model selection strategy is utilized to determine the unknown power parameter of the Bouc–Wen model. Meanwhile, optimum SVR parameters are selected automatically, instead of tuning manually. Consequently, the non‐linear structural equation is rewritten in linear form, and is solved by the SVR technique. A five‐floor versatile‐type structure is studied to show the effectiveness of the proposed method, in which both power parameter known and unknown cases are investigated. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献