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1.
论述了1:50 000松花江、嫩江干流条带图的数据格式转换、水利专题图层的设计和用JAVA语言编程调用电子地图数据的方法.  相似文献   
2.
Most of previous analyses on the active earth pressure were performed in two-dimensional cases using the Mohr-Coulomb (M-C) failure function to describe the soil strength. However, all failures of retained slopes indicate a somewhat three-dimensional (3D) feature, and the M-C function is found to overestimate the tensile strength of cohesive soil. In this work, a kinematic limit analysis–based approach is developed for computing the 3D active earth pressure resulting from cohesive backfills. The concept of tensile strength cutoff is adopted to implement the reduction or elimination of tensile strength from the strength envelope. An extended 3D horn failure mechanism that is associated with the modified strength envelope is developed to characterize the collapse of retained slopes. The resultant of active earth pressure is evaluated from the work rate balance equation and expressed as an unfactored coefficient. The obtained results indicate that less support provided by the wall is required when allowing the existence of soil cohesion and 3D effects and that eliminating the tensile strength can observably increase the active earth pressure, especially for the backfill with a great level of cohesion.  相似文献   
3.
Li  Zheng-Wei  Yang  Xiao-Li 《Acta Geotechnica》2021,16(10):3269-3283

The prediction of active earth pressure was generally implemented under the assumptions of two-dimensional conditions and cohesionless soils. However, in practice, the soils usually display a considerable level of cohesion, and the collapse of retained slopes exhibits a three-dimensional (3D) nature. Considering this fact, this paper intends to predict the 3D active earth pressure in cohesive soils based on the kinematic limit-analysis method and a 3D rotational collapse mechanism. The influence of cracks is considered, including a crack forming before the failure of retained soil masses (open crack) and a crack forming simultaneously with the failure (formation crack). The active earth pressure coefficient is estimated based on the work-energy balance principle. In order to facilitate direct application, several design charts are provided. It is shown that accounting for soil cohesion and 3D effects results in a notable decrease in the active earth pressure, whereas considering the existence of cracks would increase the pressure value. This paper develops the studies on active earth pressure, which considers the presence of cohesion, cracks, and 3D effects together for the first time. The results of this paper can offer references in designs of retaining structures for cohesive slopes.

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4.
一种水产动物病毒现场检测免疫芯片的制备与应用   总被引:4,自引:2,他引:2  
采用原子力显微镜对氨基化玻片及6种不同方法修饰玻片进行表面特征分析,并比较了硝酸纤维素(NC)膜、PVDF膜以及以上不同修饰玻片对抗体的固定效率、固定效果,最终选择琼脂糖修饰玻片作为芯片载体。将纯化后的兔抗血清(捕获抗体)点样至琼脂糖修饰玻片上,制备免疫芯片,与待检样品(病毒感染的靶器官组织)匀浆液孵育形成复合物,该复合物被辣根过氧化物酶(HRP)标记的特异性单克隆抗体(单抗)识别,经底物显色,得到肉眼可见的检测结果。通过改变芯片制备及应用过程中的具体条件参数,对各条件进行了优化,并采用生物素-链亲和素(BAS)标记特异性单抗作为检测抗体以提高检测灵敏度。基于此夹心免疫分析原理制备的WSSV、LCDV现场检测免疫芯片,病毒的最低检出量分别为82.50ng/ml、0.88μg/ml,在一定的抗原浓度范围内,病毒浓度的对数值与信号强度呈线性关系;采用BAS放大检测信号后,病毒的最低检出量为12.38ng/ml、0.22μg/ml。该免疫芯片与酶联免疫吸附法(ELISA)及免疫荧光法(IFAT)对同种样品的检测结果高度一致。  相似文献   
5.
经济的不断发展造成了极大的环境污染,需要人们用科学的手段对经济发展和造成的环境污染之间的关系进行客观判断。采用主成分分析法、格兰杰因果检验以及基于VAR的脉冲响应函数对青岛市的经济发展与环境污染二者间关系进行分析,结果发现由于环境承载力的存在,经济增长造成环境污染在很大程度上受到时滞的影响,经济增长引发环境问题是渐进的、长期的,所以在发展经济时不能只顾眼前利益而忽视对环境的长期影响,要注重可持续发展。  相似文献   
6.
鉴于弹性本构模型的简便性,目前波浪作用下海床瞬态剪切破坏深度的计算一般都是基于海床的多孔弹性介质模型,这种方法不能考虑塑性单元不平衡应力的重分配,而如果将海床视为多孔理想弹塑性介质进行波浪作用下的瞬态响应分析,则可以克服上述缺陷。为此,以波浪作用下砂质、粉砂质海床瞬态响应模型为例,基于Mohr-Coulomb屈服准则,对不同海床计算参数条件下上述2种计算方法的结果进行了对比分析。结果表明,将海床视为多孔理想弹塑性介质时海床瞬态剪切破坏深度大于等于海床为多孔弹性介质时的计算结果;随着海床计算参数的变化,2种方法计算结果的变化趋势较为一致,且在砂质、粉砂质海床常见计算参数范围内,2种方法计算结果的差值在一个相对固定的范围内变化。  相似文献   
7.
ERS-2 SAR反演海洋风矢量的研究   总被引:4,自引:0,他引:4       下载免费PDF全文
SAR(Synthetic Aperture Radar)反演海洋风矢量是当今微波遥感领域非常有意义的前沿课题. 本文首先介绍了星载SAR估算海面风向、风速的基本原理和三种主流反演算法,接着给出反演的流程图以及重要步骤. 然后,以2002年5月7日香港地区ERS-2 SAR海洋图像为例,对经典的SWDA (SAR Wind Direction Algorithm)-谱分析方法加以改进,求得具有180°模糊度的风向,并用香港天文台气象浮标实测数据消除了风向不确定性. 最后,利用CMOD4 GMF(Geophysical Model Function,地球物理模式函数)计算得到海面上10m高的风速. 与气象浮标站实测资料相比,利用ERS-2 SAR图像获取的海面风向、风速的精度均较高. 这一结果表明:如果对SAR预先进行ADC(Analog to Digital Converter)改正以及精确校准,结合改进的SWDA和CMOD4,可以获得高精度的风矢量.  相似文献   
8.
利用紫外线照射灭活的同源精子,激活栉孔扇贝(Chlamys farreri)卵子分裂,获得雌核发育胚胎,流式细胞仪检测胚胎细胞平均单倍体率为82.5%。对获得的胚胎进行全基因组扩增,获得足量的基因分型模板,利用高分辨率溶解曲线(HRM)技术对其进行96个单核苷酸多态性(SNP)位点分型。结果显示,诱导雌核发育胚胎的位点检出率为96.27%,其中99.06%的位点分型结果与相应雌性亲本相符;单个胚胎中只表现一种等位形式的位点占91.67%~96.88%,平均为94.81%,较雌性亲本的纯合位点比例(64.25%)明显提高。本研究利用灭活同源精子诱导获得栉孔扇贝雌核发育胚胎,并利用单个胚胎的全基因组扩增产物进行基因位点分析,为扇贝人工诱导雌核发育个体的早期多基因位点检测和评价提供参考。  相似文献   
9.
This study assesses the historical climate trends of surface air temperature(SAT), their spatial distributions, and the hindcast skills for SAT during 1901– 2000 from 24 Coupled Model Intercomparison Project Phase 5(CMIP5) models. For the global averaged SAT, most of the models(17/24) effectively captured the increasing trends(0.64°C/century for the ensemble mean) as the observed values(- 0.6°C/century) during the period of 1901–2000, particularly during a rapid warming period of 1970–2000 with the small model spread. In addition, most of the models(22/24) showed high hindcast skills(the correlation coefficient, R 〉 0.8). For the spatial pattern of SAT, the models better simulated the relatively larger warming at the middle-to-high latitudes in the Northern Hemisphere than that in the Southern Hemisphere and the greater warming on the land than that in the ocean between 40°S and 40°N. The simulations underestimated the warming along some ocean boundaries but overestimated warming in the Arctic Ocean. Most of the coupled models were able to reproduce the large-scale features of SAT trends in most regions excluding Antarctica, some parts of the Pacific Ocean, the North Atlantic Ocean near Greenland, the southwestern Indian Ocean, and the Arctic Ocean. The outgoing longwave radiation(OLR) and incoming shortwave radiation(ISR) at the top of the atmosphere(TOA) and the downward longwave(LW) radiation and sensible heat flux at the surface had positive contributions to the increasing trends in most of the models.  相似文献   
10.
Phenol biodegradation in an aerobic batch reactor was investigated using mixed two co-aggregating strains (Flavobacterium sp. and Acetobacter sp.). Response surface methodology by the Box–Behnken model was used to evaluate the optimal cell growth and phenol degradation conditions. The optimum temperature, pH value and inoculum size were found to be 33 °C, 6.06 and 13 %, respectively. In the conditions, phenol degradation rate and biomass were predicted to be 96.97 % and 410.78 mg/L within the range examined, respectively. Less toxic acetaldehyde, ethanol and acetic ether were identified as main intermediate products from the degraded samples using GC–MS. Substrate inhibition was calculated from experimental biomass growth and phenol degradation parameters using the Haldane equation. Kinetic parameters derived from nonlinear regression with correlation factors (R 2) were 0.9682 for phenol degradation and 0.9594 for biomass growth, respectively. The phenol concentration to avoid substrate inhibition was 278.17 mg/L.  相似文献   
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