The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish. 相似文献
High-resolution ice core records covering long time spans enable reconstruction of the past climatic and environmental conditions allowing the investigation of the earth system’s evolution. Preprocessing of ice cores has direct impacts on the data quality control for further analysis since the conventional ice core processing is time-consuming, produces qualitative data, leads to ice mass loss, and leads to risks of potential secondary pollution. However, over the past several decades, preprocessing of ice cores has received less attention than the improvement of ice drilling, the analytical methodology of various indices, and the researches on the climatic and environmental significance of ice core records. Therefore, this papers reviews the development of the processing for ice cores including framework, design as well as materials, analyzes the technical advantages and disadvantages of the different systems. In the past, continuous flow analysis (CFA) has been successfully applied to process the polar ice cores. However, it is not suitable for ice cores outside polar region because of high level of particles, the memory effect between samples, and the filtration before injection. Ice core processing is a subtle and professional operation due to the fragility of the nonmetallic materials and the random distribution of particles and air bubbles in ice cores, which aggravates uncertainty in the measurements. The future developments of CFA are discussed in preprocessing, memory effect, challenge for brittle ice, coupling with real-time analysis and optimization of CFA in the field. Furthermore, non-polluting cutters with many different configurations could be designed to cut and scrape in multiple directions and to separate inner and outer portions of the core. This system also needs to be coupled with streamlined operation of packaging, coding, and stacking that can be implemented at high resolution and rate, avoiding manual intervention. At the same time, information of the longitudinal sections could be scanned and identified, and then classified to obtain quantitative data. In addition, irregular ice volume and weight can also be obtained accurately. These improvements are recorded automatically via user-friendly interfaces. These innovations may be applied to other paleomedias with similar features and needs. 相似文献
The transfer and evolution of stress among rock blocks directly change the void ratios of crushed rock masses and affect the flow of methane in coal mine gobs. In this study, a Lagrange framework and a discrete element method, along with the soft-sphere model and EDEM numerical software, were used. The compaction processes of rock blocks with diameters of 0.6, 0.8, and 1.0 m were simulated with the degrees of compression set at 0%, 5%, 10%, 15%, 20%, and 25%. This study examines the influence of stress on void ratios of compacted crushed rock masses in coal mine gobs. The results showed that stress was mainly transmitted downward through strong force chains. As the degree of compression increased, the strong force chains extended downward, which resulted in the stress at the upper rock mass to become significantly higher than that at the lower rock mass. It was determined that under different degrees of compression, the rock mass of coal mine gobs could be divided, from the bottom to the top, into a lower insufficient compression zone (ICZ) and an upper sufficient compression zone (SCZ). From bottom to top, the void ratios in the ICZ sharply decreased and those in the SCZ slowly decreased. Void ratios in the ICZ were 1.2–1.7 times higher than those in the SCZ.
1 Introduction The Qinghai-Tibet Plateau, known as the highest plateau with the most complex topography in the world, covers an area of more than 200km2, with a mean elevation of more than 4000m a.s.l. (Ye and Gao, 1979). Surrounded by the Earth’s highest mountains, such as the Himalayas, Pamir, Kunlun Mountains, the plateau plays a significant role in climate change in China even in the world, thus attracted great attention of researchers. Up to now, many achievements have been gained by… 相似文献