Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

Hamilton系统数值计算的新方法   总被引：7，自引：0，他引：7  

廖新浩  刘林 《天文学进展》1996,14(1):3-11

系统地介绍了近年来对Ｈａｍｉｌｔｏｎ系统数值计算新建立的辛算法和线性对称多步法，并对它们在动力天文中的应用作了一简要回顾。  相似文献   

观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

关于改进的Encke方法中参考轨道的选择和校正     

刘林  胡松杰 《天文学报》1996,37(3):285-293

对于改进的Ｅｎｃｋｅ方法，选择适当的参考轨道是一个关键．然而，对于人造地球卫星长弧轨道计算，目前所给出的几种参考轨道均需要逐段校正，这将给定轨问题带来附加的复杂性．本文将仔细探讨如何选择参考轨道和减少校正次数．  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

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隧道工程地质评价的内容和方法   总被引：2，自引：0，他引：2  

陈新建  孙琳  李新星  孙巧银 《地球科学与环境学报》2004,26(1):41-45

结合工程隧道实际,首先阐述了隧道工程的基本地质环境和工程地质条件,然后针对隧道工程可能出现的不良地质现象和可采取的工程措施,从大气降水、围岩稳定、围岩压力、洞口稳定、隧道比选等角度探讨隧道工程地质评价的主要方法和一般内容,进行隧道工程地质评价,为隧道施工、支护提供了依据.  相似文献   

Microwave Type III Burst Group in the 1993 October 2 Flare     

Qijun Fu  Zhihai Qin  Yuying Liu  Guangli Huang 《Astrophysics and Space Science》1997,250(2):189-204

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.  相似文献   

STRESS CORROSION CRACKING OF AISI 321 STAINLESS STEEL IN ACIDIC SEAWATER     

黄彦良  侯保荣  曹楚南  林海潮 《中国海洋湖沼学报》1997,15(4):373-377

ImooUonONSCCisoneofthemosttwortantcausoffailureofstaiuleSsstalequipmentandfacilitieS.Studiesonunh0refacilitiesshowedthatanaddicchloridesolutionfilmcouldbefo~onthescheeofstalandthatstaiuleSSstalisinaCtivedissolutionstateinaedicchloridesolutionopinetal.,l99O;Gnanamoorthyetal.,l99O).ltisgenerallythoughtxthatSCCcannotoimrwhenthematerialisinaCtiveanodicdissolutionstate,soitsomcewithresultingcasualties(0ldfieldetal.,l99O)arousedattentiont0it.SomeSCCmeCanisrnsproposedpeuang,l988,Newmanctal.,…  相似文献   

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River     

Feng Ma  Li Liu  Lin Cao 《东北亚地学研究》2003,(1)

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …  相似文献   

Three Super Active Regions in the Descending Phase of Solar Cycle 23   总被引：2，自引：0，他引：2  

Hong-Qi Zhang  Xing-Ming Bao  Yin Zhang  Ji-Hong Liu  Shu-Dong Bao  Yuan-Yong Deng  Wei Li  Jie Chen  Jin-Ping Dun  Jiang-Tao Su  Juan Guo  Xiao-Fan Wang  Ke-Liang Hu  Gang-Hua Lin  Dong-Guang WangNational Astronomical Observatories  Chinese Academy of Sciences  Beijing   《中国天文和天体物理学报》2003,3(6):491-494

We analyze the magnetic configurations of three super active regions, NOAA 10484, 10486 and 10488, observed by the Huairou Multi-Channel Solar Telescope (MCST) from 2003 October 18 to November 4. Many energetic phenomena, such as flares (including a X-28 flare) and coronal mass ejections (CMEs), occurred during this period. We think that strong shear and fast emergence of magnetic flux are the main causes of these events. The question is also of great interest why these dramatic eruptions occurred so close together in the descending phase of the solar cycle.  相似文献