Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion     

HU Xiaoke  JIANG Xiaolu*  GUAN Huashi Fishery College  Ocean University of China  Qingdao  P..R.China 《中国海洋大学学报(英文版)》2003,2(1)

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…  相似文献   

Photopolarimetric studies of comet Austin     

U. C. Joshi  A. K. Sen  M. R. Deshpande  J. S. Chauhan 《Journal of Astrophysics and Astronomy》1992,13(3):267-277

Photopolarimetric observations of comet Austin with the IAU/IHW filter system were obtained on the 2.34 m Vainu Bappu Telescope (VBT) of the Indian Institute of Astrophysics, at Kavalur, India, during pre-perihelion phase on February 20,1990 and on the 1.2 m telescope of the Physical Research Laboratory at Gurusikhar, Mount Abu during postperihelion phase on May 2 and 4, 1990. The comet appeared bluer than a solar analog during post-perihelion phase on May 2 and 4. The percent polarization shows a sharp increase towards the red on May 2 and 4. The dominant sizes of the dust particles appear to lie in a narrow range of 0.1 to 0.5 Μm. Regarding the molecular band emission, CN and C₂ bands are quite strong; C₃ emission was also found to be strong though the observations on May 2 and 4 show significant variation as compared to C₂ emission. Molecular band polarization for CN, C₃, C₂ and H₂ O+ have been calculated. It has been found that emission polarization in CN, C₂ and C₃ is between 1–7% (phase angle between 107.4–109 degrees). For CN and C₂ the polarization values are close to the theoretically predicted values, but for C₃ the polarization value falls much below the theoretically predicted value. A similar result was found for comet Halley.  相似文献   

The efifect of wave breaking on surface wave imaging by Synthetic Aperture Radar     

孙建  管长龙  史剑 《中国海洋湖沼学报》2006,(1)

The effect of ocean wave breaking as a non-Bragg mechanism on backscattering cross-section and modulation transfer functions (MTF) of radar was investigated based on Bragg resonance theory and parametric method. The result showed that the additional effect of wave breaking on backscattering cross-section is not more than 20% except for the small incident angle of VV polarized electromagnetic (e.m.) wave but is significant for HH polarized e.m. wave. Breaking waves lead to increase in the modulus of tilt modulation MTF and the larger the wind speed, the faster the increase. For large incident angle, the modulus of tilt modulation MTF with wave breaking decreases quickly with incident angle for HH polarization and approach to that without wave breaking for VV polarization. The hydrodynamic MTF increases 30%-60% when considering wave breaking and the increase is larger for HH polarization than for VV polarization.  相似文献   

A neural network approach to ship track-keeping control     

Yao Zhang Hearn  G.E. Sen  P. 《Oceanic Engineering, IEEE Journal of》1996,21(4):513-527

This paper presents an on-line trained neural net work controller for ship track-keeping problems. Following a brief review of the ship track-keeping control development since the 1980's, an analysis of various existing backpropagation-based neural controllers is provided. We then propose a single-input multioutput (SIMO) neural control strategy for situations where the exact mathematical dynamics of the ship are not available. The aim of this study is to build an autonomous neural controller which uses rudder to regulate both the tracking error and heading error. During the whole control process, the proposed SIMO neural controller adapts itself on-line from a direct evaluation of the control accuracy, and hence the need for a “teacher” or an off-line training process can be removed. With a relatively modest amount of quantitative knowledge of the ship behavior, the design philosophy enables real time control of a nonlinear ship model under random wind disturbances and measurement noise. Three different track-keeping tasks have been simulated to demonstrate the effectiveness of the training method and the robust performance of the proposed neural control strategy  相似文献   

基于专家系统决策的多模式控制及其应用     

宣森 《广东海洋大学学报》1993,(1)

将专家系统技术与常规控制、模糊控制、超前控制、仿人智能操作控制以及计算机控制技术相结合,设计了一种新的基于专家系统决策的多模式控制(MCDBES)。经试验和实际运行结果表明,MCDBES系统对未知数学模型的被控对象,具有良好的控制性能。本文提出的MCDBES已成功地应用于燃油陶瓷窑炉计算机控制系统。  相似文献   

Local potential analysis of MHD instability     

K. K. Sen  S. J. Wilson 《Astrophysics and Space Science》1985,109(1):131-143

In many astrophysical problems, the study of the stability of an atmosphere in the presence of a magnetic field is of importance. In most cases the MHD instabilities of atmospheres are studied by energy principle of Bernsteinet al. (1958). In this paper, a general method for studying the stability of a system subject to MHD equations of conditions has been proposed. This is based on the local potential concept put forward by Glansdorff and Prigogine (1964). The scheme for securing stability criteria has been demonstrated in two particular cases.  相似文献   

钙质成岩作用对有孔虫氧同位素的影响     

房殿勇  赵泉鸿  成鑫荣  关平  李洋冰 《海洋地质与第四纪地质》2005,25(2):63-66

通过对南海北部ODPl84航次1148站井深477m之下深海相渐新世浮游有孔虫Globoquadrina压扁壳、次生方解石和充填壳的氧同位素对比，定量分析了钙质成岩作用对壳体氧同位素的影响，结果发现，受成岩作用影响，1148站浮游有孔虫氧同位素显著变轻，这种变化趋势与低纬度开放性大洋明显不同。其原因可能与南海海盆扩张早期，大量陆源物质从较窄陆架可以直接沉积到海底，由于陆源物质氧同位素相对偏负，当与海洋沉积物混合后，引起本区沉积物孔隙水氧离子浓度变轻。而扩张初始，高的沉积速率可能使钙质壳体被快速封存在一个相对孤立的系统中，造成孔隙水离子成为影响壳体氧同位素变化的主要因素。当氧同位素相对较重的有孔虫壳体溶解和重结晶并与孔隙水离子发生交换时，引起壳体δ^18O负向偏移。  相似文献   

从厌氧反应器的发展谈UASB反应器的改良   总被引：10，自引：0，他引：10  

管锡珺  郑西来 《中国海洋大学学报(自然科学版)》2004,34(2):261-268

对厌氧反应器的发展沿革、技术现状和研究进展进行了较为全面的总结。介绍了第 1代、第 2代和第 3代厌氧反应器的特点及应用状况。分析了厌氧反应器的发展趋势 ,对比了 EGSB、IC反应器与 UASB反应器的结构特征 ,提出并验证了改良 UASB反应器。结果显示 ,改良 UASB反应器有利于提高负荷和设备开发。  相似文献   

长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

紫外线照射剂量对九孔鲍胚胎及幼虫的影响     

严正凛  关瑞章  胡家财  陈晓凤 《台湾海峡》2007,26(3):404-409

采用不同的紫外线照射剂量的海水诱导九孔鲍产卵,观察其对卵子的受精率、畸形率和孵化率的影响.紫外线照射剂量的配制方法是:用紫外线照射过的最大剂量的海水与新鲜海水混合配制出各种剂量的海水.实验把雌亲鲍分成7组,每组20只,分别用紫外线照射剂量为0(对照组)、450、600、750、900、1 050和1 200mW.h/dm3的海水进行刺激产卵.试验重复3次.结果表明,紫外线照射剂量在1 200mW.h/dm3以内,对卵子的受精率影响不大;紫外线照射剂量在750mW.h/dm3以内,对卵子的畸形率影响不大,但在900~1 200mW.h/dm3,对卵子的畸形率影响很大;紫外线照射剂量在600mW.h/dm3以内,对卵子的孵化率没有什么影响,但在750~1 200mW.h/dm3,对卵子的孵化率影响很大.综合考虑卵子的受精率、畸形率和孵化率,九孔鲍催产的适宜的紫外线照射剂量应为450~750mW.h/dm3.  相似文献