Microbes live throughout the soil profile. Microbial communities in subsurface horizons are impacted by a saltwater–freshwater transition zone formed by seawater intrusion (SWI) in coastal regions. The main purpose of this study is to explore the changes in microbial communities within the soil profile because of SWI. The study characterizes the depth-dependent distributions of bacterial and archaeal communities through high-throughput sequencing of 16S rRNA gene amplicons by collecting surface soil and deep core samples at nine soil depths in Longkou City, China. The results showed that although microbial communities were considerably impacted by SWI in both horizontal and vertical domains, the extent of these effects was variable. The soil depth strongly influenced the microbial communities, and the microbial diversity and community structure were significantly different (p < 0.05) at various depths. Compared with SWI, soil depth was a greater influencing factor for microbial diversity and community structure. Furthermore, soil microbial community structure was closely related to the environmental conditions, among which the most significant environmental factors were soil depth, pH, organic carbon, and total nitrogen.
Extracting the contiguous coal seams under the lowermost aquifer in the unconsolidated Cenozoic alluvium is apt to water and quicksand inrush. By using a series of investigation methods including empirical formulas, numerical simulation, theoretical analysis, etc., the study focused on the fracture and the excess pore water pressure in the overlying strata in the process of extracting no. 8 coal seam firstly and no. 9 coal seam (under no. 8 coal seam) subsequently in no. 8102 working face of Luling coal mine in the north of Anhui Province of China. When no. 8 coal seam was extracted, the water-conducting fractured zone penetrated into the lowermost aquifer and rapid dissipation of excess pore water pressure above the gob occurred, accompanied by relatively high seepage hydraulic gradient over the headgate and the tailgate. When no. 9 coal seam was extracted, failure did not obviously extend upwards and the excess pore water pressure decreased slowly and a relatively high seepage hydraulic gradient transferred downwards from the headgate to the tailgate in the inclined profile. The safe water head (Hs) in the lowermost aquifer was confirmed to 15.6 m. Therefore, water and quicksand inrush was avoided effectively in the process of extracting the contiguous coal seams by dewatering, controlling mining height, and laying double resistance nets in the working face. 相似文献
Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I (COl) gene. A total of 76 specimens were collected from three localities around the East (Qidong and Zhoushan) and South China Seas (Zhuhai). Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity (h=0.759i0.035) and low levels of nucleotide diversity (re=0.001 98i0.003 26) were observed in these populations, Hierarchical analysis of molecular variance (AMOVA) indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant (~0~t=0.032 85, P〈0.01). These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species. 相似文献