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Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences 总被引：1，自引：0，他引：1
Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species. 相似文献
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采用紫外线(UV)灭活的同源精子通过冷休克抑制第二极体排放诱导黄姑鱼(Nibea albiflora)的雌核发育。结果表明,黄姑鱼精子最适的灭活剂量为420mJ/cm2,紫外照射剂量与黄姑鱼精子和卵子授精后的受精率及孵化率之间表现出明显的Hertwig效应。多次试验筛选的黄姑鱼雌核发育的最适条件为授精后2min于3℃处理8min。雌核发育仔鱼经形态学和流式细胞仪鉴定为二倍体。进一步利用10对微卫星分析了遗传物质在亲本和子代中的传递情况,结果表明雌核发育后代的等位基因完全来自于母本,没有父本基因参与;雌核发育后代在5个微卫星位点具有较高的重组率,使其与母本保持了高度同质性。 相似文献
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黄姑鱼雌核发育的人工诱导及鉴定 总被引：1，自引：0，他引：1
采用紫外线（UV）灭活的同源精子通过冷休克抑制第二极体排放诱导黄姑鱼的雌核发育。黄姑鱼精子最适的灭活剂量为420 mJ/cm2,紫外照射剂量与黄姑鱼精子和卵子授精后的受精率及孵化率之间表现出明显的Hertwig效应。多次试验筛选的黄姑鱼雌核发育的最适条件为授精后2min在3℃处理8min。雌核发育仔鱼经形态学和流式细胞仪鉴定为二倍体。进一步利用10对微卫星分析了遗传物质在亲本和子代中的传递情况,结果表明雌核发育后代的等位基因完全来自于母本,没有父本基因参与;雌核发育后代在5个位点微卫星位点具有较高的重组率,使其与母本保持了高度同质性。 相似文献
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