Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

THE TEMPORAL AND SPATIAL DISTRIBUTION OF SEA SURFACE WIND ALONG THE COAST OF GUANGDONG PROVINCE IN CHINA     

廖菲  邓华  陈柏纬 《热带气象学报(英文版)》2019,25(1):129-140

Temporal and spatial distribution characteristics of sea surface wind in Guangdong''s coastal areas were analyzed with data from four offshore observational stations between 2012 and 2015. The results are shown as follows: (1) The probability distribution of wind speed was basically consistent with Gaussian distribution characteristics; winds of Beaufort force 6 or higher were observed mainly in far offshore stations from October to March. (2) The probability distribution of wind direction was represented well by Weibull distribution. The deviation of wind direction of far station was relatively small for it was mainly controlled by monsoon over the South China Sea, while the near offshore station had a relatively large diurnal variation because of the influence of local synoptic systems such as sea-land breeze. (3) There were significant seasonal differences in wind speed and direction observed by different offshore observational stations. In strong wind seasons, the deviation of wind direction was relatively small while the deviation of wind speed was relatively large, and vice versa. In contrast with Class I station, the other three stations exhibited approximately normal distribution of wind direction and wind speed deviations. (4) Wind direction diurnal variation was moderate in windy periods, while it was obvious in relatively lower speed conditions. The deviation of wind speed in windy periods was generally greater because it was influenced by mesoscale weather systems for 10-20 h, and the influence was complicated, resulting in greater local differences in wind speed.  相似文献   

Increasing Singapore's resilience to drought          下载免费PDF全文

Alan D. Ziegler  James P. Terry  Grahame J.H. Oliver  Daniel A. Friess  Chong Joon Chuah  Winston T.L. Chow  Robert J. Wasson 《水文研究》2014,28(15):4543-4548

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Estimation of flood damage functions for river basin planning: a case study in Bangladesh     

Y. C. Ethan Yang  Patrick A. Ray  Casey M. Brown  Abedalrazq F. Khalil  Winston H. Yu 《Natural Hazards》2015,75(3):2773-2791

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Reconstructed light extinction coefficients using chemical compositions of PM<Subscript>2.5</Subscript> in winter in Urban Guangzhou,China   总被引：8，自引：0，他引：8  

陶俊  曹军骥  张仁健  朱李华  张涛  施思  陈尊裕 《大气科学进展》2012,29(2):359-368

The objective of this study was to reconstruct light extinction coefficients (b ext ) according to chemical composition components of particulate matter up to 2.5 μm in size (PM 2.5 ). PM 2.5 samples were collected at the monitoring station of the South China of Institute of Environmental Science (SCIES, Guangzhou, China) during January 2010, and the online absorbing and scattering coefficients were obtained using an aethalometer and a nephelometer. The measured values of light absorption coefficient by particle (b ap ) and light scattering coefficient by particle (b sp ) significantly correlated (R 2 > 0.95) with values of b ap and b sp that were reconstructed using the Interagency Monitoring of Protected Visual Environments (IMPROVE) formula when RH was <70%. The measured b ext had a good correlation (R 2 > 0.83) with the calculated b ext under ambient RH conditions. The result of source apportionment of b ext showed that ammonium sulfate [(NH 4 ) 2 SO 4 ] was the largest contributor (35.0%) to b ext , followed by ammonium nitrate (NH 4 NO 3 , 22.9%), organic matter (16.1%), elemental carbon (11.8%), sea salt (4.7%), and nitrogen dioxide (NO 2 , 9.6%). To improve visibility in Guangzhou, the effective control of secondary particles like sulfates, nitrates, and ammonia should be given more attention in urban environmental management.  相似文献   

安徽濉溪邹楼铁矿物探异常特征     

产思维  缪旭煌 《江苏地质》2017,41(1):134-137

安徽省濉溪县邹楼铁矿为接触交代型铁矿床,矿床地质特征与华北地块“邯邢式”铁矿类似,矿体位于燕山期闪长质侵入岩与下奥陶统萧县组碳酸盐岩接触带附近,矿床规模较小,矿体个数多。邹楼铁矿物探异常特征较为明显,处于重力高、磁力高、电阻率等值线低阻扭曲部位。通过对该矿床的地质、物探异常特征进行总结,以期为该区乃至华北地块其他地区寻找类似矿床提供借鉴。  相似文献   

基于风廓线雷达观测的台风“天兔”边界层演变特征研究     

廖菲  邓华  高志球  陈伯纬 《海洋学报(英文版)》2017,36(9):39-44

垂直探测台风边界层特征对于认清台风结构具有重要意义。基于1319号台风“天兔”途经的三个边界层风廓线雷达站的观测资料,结合探空数据,本文分析了“天兔”的边界层径向、切向风特征,结果表明：1）最大切向风速出现在眼区附近,在“天兔”稳定维持为强台风级别期间,最大切向风速基本稳定在1800 m高度左右,随着登陆后强度的迅速减弱,最大切向风速减弱、最大切向风速垂直范围缩小;2）最大风速所在高度和台风入流层顶高基本相近,大于依据理查森数或位温梯度所判断出的边界层高度,而基于信噪比（SNR）或其梯度所判断的混合层高度时常偏低;3）“天兔”登陆前边界层高度可以达到2100 m以上,在台风级别及以上时,各站观测到的边界层高度变化不大,基本在1200～1600 m之间,登陆后随着台风强度的减弱,边界层高度迅速减小。  相似文献   

Aircraft vertical profiling of variation of CO₂ over a Canadian Boreal Forest Site: a role of advection in the changes in the atmospheric boundary layer CO₂ content     

ALEXANDER SHASHKOV  KAZ HIGUCHI  DOUGLAS CHAN 《Tellus. Series B, Chemical and physical meteorology》2007,59(2):234-243

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Multi-factor impact analysis of agricultural production in Bangladesh with climate change   总被引：1，自引：0，他引：1  

Alex C. Ruane  David C. Major  Winston H. Yu  Mozaharul Alam  Sk. Ghulam Hussain  Abu Saleh Khan  Ahmadul Hassan  Bhuiya Md. Tamim Al Hossain  Richard Goldberg  Radley M. Horton  Cynthia Rosenzweig 《Global Environmental Change》2013,23(1):338-350

Diverse vulnerabilities of Bangladesh's agricultural sector in 16 sub-regions are assessed using experiments designed to investigate climate impact factors in isolation and in combination. Climate information from a suite of global climate models (GCMs) is used to drive models assessing the agricultural impact of changes in temperature, precipitation, carbon dioxide concentrations, river floods, and sea level rise for the 2040–2069 period in comparison to a historical baseline. Using the multi-factor impacts analysis framework developed in Yu et al. (2010), this study provides new sub-regional vulnerability analyses and quantifies key uncertainties in climate and production. Rice (aman, boro, and aus seasons) and wheat production are simulated in each sub-region using the biophysical Crop Environment REsource Synthesis (CERES) models. These simulations are then combined with the MIKE BASIN hydrologic model for river floods in the Ganges-Brahmaputra-Meghna (GBM) Basins, and the MIKE21 Two-Dimensional Estuary Model to determine coastal inundation under conditions of higher mean sea level. The impacts of each factor depend on GCM configurations, emissions pathways, sub-regions, and particular seasons and crops. Temperature increases generally reduce production across all scenarios. Precipitation changes can have either a positive or a negative impact, with a high degree of uncertainty across GCMs. Carbon dioxide impacts on crop production are positive and depend on the emissions pathway. Increasing river flood areas reduce production in affected sub-regions. Precipitation uncertainties from different GCMs and emissions scenarios are reduced when integrated across the large GBM Basins’ hydrology. Agriculture in Southern Bangladesh is severely affected by sea level rise even when cyclonic surges are not fully considered, with impacts increasing under the higher emissions scenario.  相似文献   

MODFLOW--一套水文地质学实用计算软件     

蒋亚萍  陈余道 《南方国土资源》1999,12(3):75-78

MODFLOW是水文地质学领域内一套实用的计算软件,文章对它的特点与应用、总体结构以及所包含的子程序包作了介绍,阐述了该软件的接触方式和在我国推广的必要性.  相似文献