Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

尼泊尔8.1级地震对中国西藏地区造成的震害特征分析   总被引：1，自引：0，他引：1  

高锦瑞  尼玛  文升梁  张建龙 《震灾防御技术》2015,10(4):961-968

2015年4月25日尼泊尔发生8.1级地震,对我国西藏地区造成较大人员伤亡与房屋破坏,道路、通讯等生命线工程及水利等基础设施损坏严重。本文介绍了本次地震的基本情况,并在现场地震烈度调查和地震损失评估的基础上,对灾区震害进行了分析,给出了灾区房屋类别与破坏情况以及生命线系统与各行业的受损情况。通过分析此次地震的灾害特点,指出了灾区在抗震设防中存在的问题,并提出加大地震地质灾害的防治力度,科学编制恢复重建规划,加强防震减灾宣传,提高农牧民抗震设防意识,加强农牧区房屋建筑的指导和监管等建议,以减少地震造成的人员伤亡和财产损失,促进西藏地区经济和社会的和谐发展。  相似文献