Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

城区水文循环机制与改善策略分析          下载免费PDF全文

廖朝轩  詹丽梅  陈家梁  邱奕儒 《水科学进展》2008,19(1):49-53

在城区化过程中,由于人口快速成长且集中,随之而来的建筑物、道路等不透水面密度增加,导致地下水补给减少及破坏水文循环。为了解土地利用方式改变对长期水文量影响,建立一个适宜的城区水循环模式是非常重要的。选定台湾台北的六馆抽水站集水区进行分析,模式参数经过敏感度分析、率定与验证。采用改变不同透水铺面设置比例的方案,评估其对径流量、入渗量、蒸发散量的改变。并分析设置不同比例屋顶雨水贮集系统对地表径流量及供水量的改变。所建立的城区水循环模式是评估土地利用改变造成水文量的改变的一个有效工具,并可提供决策者做为城区土地利用决策的参考。  相似文献   

中国中西部地区地震的重新定位和三维地壳速度结构   总被引：8，自引：1，他引：8       下载免费PDF全文

杨智娴  于湘伟  郑月军  陈运泰  倪晓晞  Winston CHAN 《地震学报》2004,26(1):19-29

采用中国中西部地区(21~36N， 98~112E)193个地震台在1992～1999年间记录到的9 988次地震的Pg和Sg震相走时的读数资料，用Roecker的SPHYPIT90程序，反演了该地区三维地壳P波速度结构，并用SPHREL3D90程序进行了地震的重新定位．反演结果揭示了中国中西部地区地震P波速度结构明显的横向不均匀性，这些不同深度上波速的横向变化多以该地区的活动断裂为分界线．可以看出活动断裂两侧存在明显的速度反差．通过重新定位，得到了6 459次地震的震源参数，这些精确定位的地震震中明显沿该区活动断裂呈现条带状分布，其范围和尺度清晰地表示了这一地区地震活动与活动断裂的紧密关系．其中，82％重新精确定位的事件的震源深度在20 km以内. 这一结果与笔者用双差地震定位法得到的重新定位的震源深度分布相一致．   相似文献   

On the CO₂ exchange between the atmosphere and the biosphere: the role of synoptic and mesoscale processes     

DOUGLAS CHAN  CHIU WAI YUEN  KAZ HIGUCHI  ALEXANDER SHASHKOV  JANE LIU  JING CHEN  DOUGLAS WORTHY 《Tellus. Series B, Chemical and physical meteorology》2004,56(3):194-212

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Earthquake relocation and 3-dimensional crustal structure of P-wave velocity in cen-tral-western China     

杨智娴  于湘伟  郑月军  陈运泰  倪晓晞  Winston CHAN 《地震学报(英文版)》2004,17(1):20-30

IntroductionUnderstandingthemechanismofcontinentalearthquakesisveryimportantforseismichaz-ardpreventionandearthquakeprediction.Themodernseismotectonictheoryandtheideaofearthquakepredictionaredevelopedmainlyfromthestudiesoninterplateearthquakes,whicharedifficulttoexplainthephenomenaofintraplateearthquakes,suchasthecontinentalearthquakesoccurredinChinesemainland.Whiletheinterplateearthquakesoccurredalongtheplatebounda-ries,theintraplateearthquakesdistributediffuselyintheinterioroftheplates.Thus…  相似文献   

Vertical structures of PM10 and PM 2.5 and their dynamical character in low atmosphere in Beijing urban areas     

DING Guoan  CHAN Chuenyu  GAO Zhiqiu  YAO Wenqing  LI Yoksheung  CHENG Xinghong  MENG Zhaoyang  YU Haiqing  WONG Kamhang  WANG Shufeng  MIAO Qiuju 《中国科学D辑(英文版)》2005,48(Z2)

The vertical structures and their dynamical character of PM2.5 and PM10 over Beijing urban areas are revealed using the 1 min mean continuous mass concentration data of PM2.5 and PM10 at 8, 100, and 320 m heights of the meteorological observation tower of 325 m at Institute of Atmospheric Physics, Chinese Academy of Sciences (IAP CAS tower hereafter) on 10―26 August, 2003, as well as the daily mean mass concentration data of PM2.5 and PM10 and the continuous data of CO and NO2 at 8, 100 (low layer), 200 (middle layer), and 320 m (high layer) heights, in combination with the same period meteorological field observation data of the meteorological tower. The vertical distributions of aerosols observed on IAP CAS tower in Beijing can be roughly divided into two patterns: gradually and rapidly decreasing patterns, I.e. The vertical distribution of aerosols in calm weather or on pollution day belongs to the gradually decreasing pattern, while one on clean day or weak cold air day belongs to the rapidly decreasing pattern. The vertical distributive characters of aerosols were closely related with the dynamical/thermal structure and turbulence character of the atmosphere boundary layer. On the clean day, the low layer PM2.5 and PM10 concentrations were close to those at 8 m height, while the concentrations rapidly decreased at the high layer, and their values were only one half of those at 8 m, especially, the concentration of PM2.5 dropped even more. On the clean day, there existed stronger turbulence below 150 m, aerosols were well mixed, but blocked by the more stronger inversion layer aloft, and meanwhile, at various heights, especially in the high layer, the horizontal wind speed was larger, resulting in the rapid decrease of aerosol concentration, I.e. Resulting in the obvious vertical difference of aerosol concentrations between the low and high layers. On the pollution day, the concentrations of PM2.5 and PM10 at the low, middle, and high layers dropped successively by, on average, about 10% for each layer in comparison with those at 8 m height. On pollution days, in company with the low wind speed, there existed two shallow inversion layers in the boundary layer, but aerosols might be, to some extent, mixed below the inversion layer, therefore, on the pollution day the concentrations of PM2.5 and PM10 dropped with height slowly; and the observational results also show that the concentrations at 320 m height were obviously high under SW and SE winds, but at other heights, the concentrations were not correlated with wind directions. The computational results of footprint analysis suggest that this was due to the fact that the 320 m height was impacted by the pollutants transfer of southerly flow from the southern peripheral heavier polluted areas, such as Baoding, and Shijiazhuang of Hebei Province, Tianjin, and Shandong Province, etc., while the low layer was only affected by Beijing's local pollution source. The computational results of power spectra and periods preliminarily reveal that under the condition of calm weather, the periods of PM10 concentration at various heights of the tower were on the order of minutes, while in cases of larger wind speed, the concentrations of PM2.5 and PM10 at 320 m height not only had the short periods of minute-order, but also the longer periods of hour order. Consistent with the conclusion previously drawn by Ding et al., that air pollutants at different heights and at different sites in Beijing had the character of "in-phase" variation, was also observed for the diurnal variation and mean diurnal variation of PM2.5 and PM10 at various heights of the tower in this experiment, again confirming the "in-phase" temporal/spatial distributive character of air pollutants in the urban canopy of Beijing. The gentle double-peak character of the mean diurnal variation of PM2.5 and PM10 was closely related with the evident/similar diurnal variation of turbulent momentum fluxes, sensible heat fluxes, and turbulent kinetic energy at various heights in the urban canopy. Besides, under the condition of calm weather, the concentration of PM2.5 and PM10 declined with height slowly, it was 90% of 8 m concentration at the low layer, a little lesser than 90% at the middle layer, and 80% at the high layer, respectively. Under the condition of weak cold air weather, the concentration remarkably dropped with height, it was 70% of 8 m concentration at the low layer, and 20%―30% at the middle and high layers, especially the concentration of PM2.5 was even lower.  相似文献   

香港世界地质公园晚侏罗世荔枝庄组岩石组合及其成岩地质过程解析     

姜杨  杨家明  杨祝良  沈加林  马雪  陈羽岚  钱迈平  邢光福  余明刚  段政 《地质论评》2016,62(3):759-769

中国东南大陆晚侏罗世地层普遍缺失,仅零星见于个别地区,香港新界东北的荔枝庄组即为其一。荔枝庄组出露于香港世界地质公园沉积岩园区的荔枝庄地区,自下往上由火山岩—沉积岩—火山岩组合而成,沉积岩中发育大型包卷层理和滑塌构造等典型沉积构造,是香港地区最具代表性的晚侏罗世火山—沉积岩系。通过实测地层剖面研究,确定其成岩过程大体上可划分为早期普林尼式火山爆发、中期破火山口湖相沉积和晚期普林尼式火山爆发三个阶段,以湖相沉积作用为主、火山喷发作用为辅;受晚期火山岩浆活动的影响,沉积岩层普遍发生硅化或炭化。荔枝庄组独特的岩石组合与形成的古地理环境,为探讨中国东南大陆中生代火山活动—沉积作用方式与成岩过程,提供了难得的研究实例。  相似文献   

THE TEMPORAL AND SPATIAL DISTRIBUTION OF SEA SURFACE WIND ALONG THE COAST OF GUANGDONG PROVINCE IN CHINA     

廖菲  邓华  陈柏纬 《热带气象学报(英文版)》2019,25(1):129-140

Temporal and spatial distribution characteristics of sea surface wind in Guangdong''s coastal areas were analyzed with data from four offshore observational stations between 2012 and 2015. The results are shown as follows: (1) The probability distribution of wind speed was basically consistent with Gaussian distribution characteristics; winds of Beaufort force 6 or higher were observed mainly in far offshore stations from October to March. (2) The probability distribution of wind direction was represented well by Weibull distribution. The deviation of wind direction of far station was relatively small for it was mainly controlled by monsoon over the South China Sea, while the near offshore station had a relatively large diurnal variation because of the influence of local synoptic systems such as sea-land breeze. (3) There were significant seasonal differences in wind speed and direction observed by different offshore observational stations. In strong wind seasons, the deviation of wind direction was relatively small while the deviation of wind speed was relatively large, and vice versa. In contrast with Class I station, the other three stations exhibited approximately normal distribution of wind direction and wind speed deviations. (4) Wind direction diurnal variation was moderate in windy periods, while it was obvious in relatively lower speed conditions. The deviation of wind speed in windy periods was generally greater because it was influenced by mesoscale weather systems for 10-20 h, and the influence was complicated, resulting in greater local differences in wind speed.  相似文献   

Determination of Carbonate Carbon in 41 International Geochemical Reference Samples by Coulometric Method     

Chris C.Y. CHAN 《Geostandards and Geoanalytical Research》1986,10(2):131-134

The carbonate-carbon (CO₂) content of forty-one geochemical reference samples has been determined by coulometric method following acid treatment of the sample for releasing CO₂. The method is superior to the conventional methods in speed, accuracy, sensitivity, specificity, and the coverage of CO₂ range. The results on NBS limestone samples agree well with the certified values. The precision of the method is 0.5 % r.s.d., and the practical detection limit is 10 ppm C.  相似文献   

大庆油田葡萄花油层组储层非均质性主控因素分析   总被引：6，自引：0，他引：6       下载免费PDF全文

单敬福  纪友亮 《沉积与特提斯地质》2006,26(4):95-100

本文对萨尔图油田中部葡萄花油层组的非均质性进行定量半定量研究,认为葡萄花油层组PⅠ2小层层内非均质性最严重,与沉积微相密切相关;单砂层垂向上渗透率的变化以正韵律和复合式韵律为主,局部发育反韵律模式;层内非均质性强弱依次为PⅠ2a、PⅠ3、PⅠ2b、PⅠ1、PⅠ4;层间非均质性在PⅠ3和PⅠ4间表现得最强,其它相对要弱;各小层平面非均质性相差无几,整体表现为较强的非均质性。  相似文献