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Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance.However,the identification of new varieties of shrimps from their phenotypic characters is difficult.This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences.The method was employed to identify a new selected variety,Kehai No.1(KH-1),from three representative stocks(control group):Zhengda;Tongwei;and a stock collected from Fujian Province,which is now cultured in mainland China.By pooled genotyping of KH-1 and the control group,five microsatellites showing differences between KH-1 and the control group were screened out.Individual genotyping data confirmed the results from pooled genotyping.The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2.By sequencing the mitochondrial control regions of individuals from the KH-1 and control group,four haplotypes were observed in the KH-1 group,whereas14 haplotypes were obtained in the control group.By combining the microsatellite assignment analysis with mitochondrial control region analysis,the average accuracy of identification of individuals in the KH-1group and control group reached 89%.The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L.vannamei from other populations cultured in China. 相似文献
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基于转录组数据的凡纳滨对虾微卫星标记开发 总被引：2，自引：0，他引：2
对虾遗传多样性和育种研究需要大量的分子标记。作者利用凡纳滨对虾(Litopenaeus vannamei)转录组测序数据,采用MISA软件进行微卫星序列挖掘,共获得14 767条微卫星序列。统计发现在凡纳滨对虾中微卫星出现的频率为16.76%;在所有的微卫星序列中,2碱基微卫星最多,占59.53%;其次是3碱基微卫星,占35.78%。随机选取其中74条序列设计引物,通过DNA混池扩增和分型的方法进行微卫星标记的开发,PCR扩增的显示有54对(72.97%)能扩增出清晰的目的条带,进一步分型的结果显示27条(36.49%)微卫星显示出多态性,从这些多态性的微卫星位点中选择11个位点在"科海1号"种质库材料中进行个体分型,结果显示在该群体中微卫星标记的等位基因数目为2~11个不等,期望杂合度值为0.256~0.858,观察杂合度为0.213~0.875,多态性信息含量为0.221~0.830,在11个位点中有4个位点显著偏离HWE(P0.05)。本研究结果为后续使用微卫星标记进行对虾遗传学研究,促进对虾的遗传选育和种质资源保护提供了重要基础。 相似文献
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