生物传感技术在海洋监测中的应用   总被引：1，自引：0，他引：1  

尹秀丽  薛钦昭  秦伟 《海洋科学》2011,35(8):113-118

21世纪是海洋开发的世纪,我国将更加关注在可持续发展前提下的海洋资源的合理开发和海洋环境保护,特别是更多地关注近海生态环境的监测和保护。用于海洋监测的生物传感器技术是一种基于生物敏感元件对污染物的生理、生化、细胞反应而建立起来的海洋生物监测技术,有很高的灵敏度,并可与生物个体的生长、繁殖、代谢、存活等指标直接联系起来,...  相似文献   

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics     

吉成龙  吴惠丰  刘小莉  赵建民  于君宝  尹秀丽 《中国海洋湖沼学报》2013,31(2):345-352

Ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine contaminants.We investigated the influence of dilution to 75% and 50% of normal seawater salinity(31.1) on the responses of the digestive gland of R.philippinarum to arsenic exposure(20 μg/L),using nuclear magnetic resonance(NMR)-based metabolomics.After acute arsenic exposure for 48 h,salinity-dependent differential metabolic responses were detected.In normal seawater,arsenic exposure increased the concentrations of branched-chain amino acids,and of threonine,proline,phosphocholine and adenosine,and it decreased the levels of alanine,hypotaurine,glucose,glycogen and ATP in the digestive glands.Differential changes in metabolic biomarkers observed at lower salinity(～23.3) included elevation of succinate,taurine and ATP,and depletion of branched-chain amino acids,threonine and glutamine.Unique effects of arsenic at the lowest salinity(～15.6) included down-regulation of glutamate,succinate and ADP,and up-regulation of phosphocholine.We conclude that salinity influences the metabolic responses of this clam to arsenic.  相似文献