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A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has beenisolated and extracted from a Cell=free preparation of Spirulina platensis.The effect of the s-factor in the re-action system is similar to that of Na_2S_2O_4, but is coupled with ligh. The s-factor has the maximumabsorption peak at 620 nm in the oxidized state, at 590 nm in the reduced state. The partially purifieds-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin,which has nochange of oxidized state and reduced state absorption spectra, and also has no promoting effect onhydrogenase of Spirulina platensis under the light. 相似文献
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螺旋藻氢酶的制备及其特性的研究 总被引：1，自引：0，他引：1
氢是理想的不污染环境的能源,在世界能源日趋紧张的情况下,氢代谢这一研究课题受到了许多学者的重视。藻类中一些绿藻可以放氢已有不少研究;关于蓝藻的放氢,特别是不具有异形胞蓝藻的放氢研究目前报道得很少。螺旋藻是一种不能固氮的丝状蓝藻,但具有氢酶。顾天青等已进行了螺旋藻整体细胞放氢特性的研究,证明了氢酶与硝酸还原酶之间存在还原能力的竞争。在此基础上,我们提取了螺旋藻的氢酶,在分子水平上对它的放氢特性进行了研究,并以Na_2S_2O_4还原的甲基紫精作为催化放氢的试验。 相似文献
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