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DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea 总被引：1，自引：0，他引：1
A total of 142 specimens of Ceramiales(Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species,14 genera,and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers,namely,partial large subunit r RNA gene(LSU),universal plastid amplicon(UPA),and partial mitochondrial cytochrome c oxidase subunit I gene(COI). No intraspecific divergence was found in our collection for these markers,except for a 1–3 bp divergence in the COI of Ceramium kondoi,Symphyocladia latiuscula,and Neosiphonia japonica. Because short DNA markers were used,the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in Gen Bank or BOLD(Barcode of Life Data) Systems. Three specimens were presumed as H eterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification,which was the first record in the investigated area,implying that it might be a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers,H eterosiphonia japonica converged with D asya spp. and was distant from the other Heterosiphonia spp.,implying that H. japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species,but the COI had a higher ability to discriminate between species. 相似文献
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DNA条形码是指利用一段相对较短的标准DNA片段,对物种进行识别和鉴定。目前该技术在动物、植物物种鉴定领域已经得到了广泛的研究和应用。在藻类的研究中,尚未确定一条统一的标准条形码基因,现阶段都是使用2条或2条以上基因序列来完成物种鉴定。对于形态多样、种类繁多的海洋红藻,常用的DNA条形码基因有COI基因(Partial cytochrome c oxidase I gene)、UPA基因(Partial 23SrRNA gene,universal plastid amplicon)、LSU基因(Partial 28SrRNA gene)和rbcL基因(The large subunit of ribulose-1,5-bisphosphate carboxylase)等,这些基因中2个或3个基因的互补运用准确有效地提高了红藻的鉴定准确率,尤其是COI基因的种间差异大足够区分相近物种。本文在概述条形码的原理及其标准的基础上,阐述了红藻DNA条形码鉴定研究的新进展以及常用几种基因片段的优缺点,并对条形码在红藻鉴定中存在的问题进行了分析,对其应用前景进行了展望。 相似文献
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Behavioral and physiological responses to light are the two major mechanisms by which natural microphytobenthic assemblages adapt to the intertidal environment and protect themselves from light stress. The present study investigated these photoresponses with diff erent light intensities over 8 h of illumination, and used a specific inhibitor(Latrunculin A, Lat A) for migration to compare migratory and non-migratory microphytobenthos(MPB). Photosynthetic activity was detected using rapid light curves and induction curves by chlorophyll fluorescence. It showed distinct variation in migratory responses to diff erent light intensities; high light induced downward migration to avoid photoinhibition, and low and medium light(50–250 μmol/(m~2 ·s)) promoted upward migration followed by downward migration after certain period of light exposure. No significant difference in non-photochemical quenching(NPQ) or PSII maximal quantum yield(F v/F m) was detected between low and medium light irradiance, possibly indicating that only high light influences the photosynthetic capability of MPB. Decreased photosynthetic activity, indicated by three parameters, the maximum relative electron transport rate(r ETR_(max)), minimum saturating irradiance(E _k) and light utilization coefficient(α), was observed in MPB after exposure to prolonged illumination under low and medium light. Lat A ef fectively inhibited the migration of MPB in all light treatments and induced lower F v/F _m under high light(500 and 100 μmol/(m~2 ·s)) and prolonged illumination at 250 μmol/(m~2 ·s), but did not significantly influence F _v/F m under low light(0–100 μmol/(m~2 ·s)) or NPQ. The increase of NPQ in Lat A treatments with time implied that the MPB assemblages can recover their physiological photoprotection capacity to adapt to light stress. Non-migratory MPB exhibited lower light use efficiency(lower α) and lower maximum photosynthetic capacity(lower r ETR_(max)) than migratory MPB under light intensities above 250 μmol/(m ~2 ·s) after 4.0 h illumination. 相似文献
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基于河口潮间带底栖微藻群落水平的环境质量状态评价 总被引：1，自引：1，他引：0
The feasibility of community-based bioassessment of environmental quality status was studied using microphytobenthos(MPB) in estuarine intertidal ecosystems.The sediment samples of MPB were collected monthly during a 1-year cycle(September 2006–August 2007) at four sampling stations in the Nakdong River Estuary,Korea.Environmental variables,such as salinity,radiation,grain size of sediment,Si(OH)_4(Si),nitrate(NO_3~–),nitrite(NO_2~–),ammonium(NH_4~+) and phosphates(PO_4~(3–)),were measured synchronously for comparison with biotic parameters.The statistical analyses were carried out for assessment the relationship between biotic and environmental parameters.The results showed that:(1) the MPB community structures were significant differences among four sampling stations;(2) spatial variation in the MPB communities were significantly correlated with environmental variables,especially the nutrient NH_4+ in combination with salinity and grain size;(3) three species(Navicula lacustris,Pleurosigma anglulatum and Fragilaria sp.1) were significantly correlated with nutrients and/or Si;and(4) the species richness and diversity were significantly correlated with the grain size.It is suggested that MPB communities may be used as a potentially robust bioindicator for assessing environmental quality status in estuarine intertidal ecosystems. 相似文献
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